EVI5 (NM_005665) Human Tagged ORF Clone

CAT#: RC223120

EVI5 (Myc-DDK-tagged)-Human ecotropic viral integration site 5 (EVI5)

Product Images

Circular map for RC223120

• 

  Western blot validation of overexpression lysate (Cat# LY401728) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223120 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EVI5
• Synonyms: EVI-5; NB4S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223120 representing NM_005665
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTACCAACAAAATGACTGCTGCCTTTAGAAACCCTAGTGGGAAACAGGTGGCGACAGACAAAGTTG
  CAGAAAAGCTGAGCTCTACTCTCTCATGGGTGAAGAACACAGTATCGCATACAGTCAGTCAGATGGCCAG
  TCAGGTGGCAAGTCCATCTACTTCATTACATACCACATCCTCATCTACCACACTATCAACACCAGCCCTT
  TCACCATCTTCCCCATCACAGTTGAGTCCAGACGACTTAGAACTCCTGGCTAAACTGGAAGAACAGAATA
  GATTGTTAGAAACGGATAGTAAGTCTTTAAGATCTGTAAATGGGTCAAGAAGAAACAGTGGCTCTTCTCT
  TGTGTCGAGTTCATCAGCCTCTAGCAACCTCAGTCACCTTGAAGAAGATTCTTGGATTCTTTGGGGAAGA
  ATTGTTAATGAATGGGAAGATGTACGCAAAAAGAAGGAAAAGCAAGTTAAGGAACTTGTTCATAAAGGGA
  TACCCCATCACTTTAGAGCAATAGTTTGGCAACTTTTATGCAGTGCACAAAGTATGCCAATTAAGGATCA
  GTATTCAGAACTCCTGAAAATGACCTCGCCTTGTGAAAAATTGATCCGAAGGGACATTGCTAGAACTTAC
  CCTGAACACAACTTTTTTAAGGAAAAAGATAGCCTTGGACAGGAGGTTTTATTTAATGTAATGAAGGCTT
  ACTCTTTAGTAGATCGTGAGGTTGGTTACTGTCAAGGAAGTGCTTTTATAGTTGGATTGTTGCTTATGCA
  GATGCCAGAAGAAGAAGCTTTCTGTGTATTTGTTAAATTAATGCAAGATTATAGACTTCGTGAACTTTTT
  AAACCAAGTATGGCAGAATTGGGCCTTTGTATGTACCAGTTTGAATGTATGATACAGGAGCATCTTCCAG
  AGCTCTTTGTACATTTTCAATCTCAGAGTTTTCATACCTCAATGTATGCATCATCCTGGTTTCTGACTAT
  CTTTCTTACAACTTTTCCACTACCAGTTGCAACAAGGATATTTGATATCTTTATGTCTGAGGGTTTAGAA
  ATAGTGTTTCGTGTAGGATTAGCACTTCTTCAGATGAATCAGGCAGAACTGATGCAACTTGACATGGAAG
  GGATGTTACAGCACTTTCAAAAGGTCATTCCACATCAGTTTGATGGTGTCCCAGACAAGCTAATCCAAGC
  AGCTTACCAAGTCAAATACAATTCAAAAAAAATGAAAAAGCTTGAAAAGGAATACACTACAATAAAAACG
  AAAGAAATGGAAGAGCAAGTTGAAATTAAAAGGTTACGCACAGAAAATAGACTTTTAAAACAGCGCATCG
  AGACATTAGAAAAACATAAATGCAGTTCCAACTACAACGAAGATTTTGTGCTACAGCTAGAGAAGGAATT
  GGTCCAAGCCCGACTGAGTGAAGCTGAGTCTCAGTGTGCATTAAAAGAGATGCAGGATAAAGTCTTGGAT
  ATAGAGAAGAGGAATAACTCCCTTCCTGATGAGAATAATATTGCAAGGCTTCAGGAAGAACTCATTGCTG
  TGAAACTTAGAGAAGCAGAAGCCATTATGGGTTTGAAAGAACTTAGACAGCAAGTCAAGGATTTAGAGGA
  ACACTGGCAGCGCCACTTAGCTCGTACTACTGGGAGATGGAAAGACCCACCCAAGAAAAATGCTATGAAT
  GAGTTACAGGATGAACTGATGACCATTCGACTTAGAGAAGCTGAAACACAAGCAGAAATAAGAGAAATAA
  AACAAAGGATGATGGAAATGGAAACACAGAACCAGATCAATAGTAACCATCTTCGAAGAGCAGAACAAGA
  GGTGATTAGCCTACAGGAGAAAGTGCAGTATCTTTCTGCACAGAACAAAGGACTCCTTACTCAATTAAGT
  GAAGCAAAGCGTAAACAAGCAGAGATTGAATGCAAGAATAAGGAAGAAGTGATGGCTGTGAGGCTTCGGG
  AAGCAGATAGCATAGCTGCTGTGGCTGAACTACGACAACACATTGCTGAGCTTGAAATCCAGAAAGAAGA
  AGGAAAGCTTCAAGGACAGCTTAACAAGTCTGATTCTAACCAGTATATTGGGGAACTGAAAGATCAGATA
  GCAGAGCTGAATCATGAGCTCAGGTGCCTAAAAGGCCAGAGGGGCTTCTCAGGCCAACCTCCTTTTGATG
  GAATCCACATTGTCAACCATTTAATAGGAGATGATGAATCATTCCATTCCTCCGATGAAGATTTTATAGA
  TAATTCCTTACAGGAAACTGGTGTTGGTTTTCCTTTGCACGGAAAATCTGGCTCGATGTCTTTGGACCCC
  GCAGTGGCAGATGGTAGTGAGAGCGAAACAGAAGACAGTGTGCTGGAGACCAGAGAGAGCAACCAAGTGG
  TTCAAAAGGAGCGGCCCCCGAGAAGAAGAGAGTCGTATTCAACCACTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223120 representing NM_005665
  Red=Cloning site Green=Tags(s)
  
  MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTSSSTTLSTPAL
  SPSSPSQLSPDDLELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSSSSASSNLSHLEEDSWILWGR
  IVNEWEDVRKKKEKQVKELVHKGIPHHFRAIVWQLLCSAQSMPIKDQYSELLKMTSPCEKLIRRDIARTY
  PEHNFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELF
  KPSMAELGLCMYQFECMIQEHLPELFVHFQSQSFHTSMYASSWFLTIFLTTFPLPVATRIFDIFMSEGLE
  IVFRVGLALLQMNQAELMQLDMEGMLQHFQKVIPHQFDGVPDKLIQAAYQVKYNSKKMKKLEKEYTTIKT
  KEMEEQVEIKRLRTENRLLKQRIETLEKHKCSSNYNEDFVLQLEKELVQARLSEAESQCALKEMQDKVLD
  IEKRNNSLPDENNIARLQEELIAVKLREAEAIMGLKELRQQVKDLEEHWQRHLARTTGRWKDPPKKNAMN
  ELQDELMTIRLREAETQAEIREIKQRMMEMETQNQINSNHLRRAEQEVISLQEKVQYLSAQNKGLLTQLS
  EAKRKQAEIECKNKEEVMAVRLREADSIAAVAELRQHIAELEIQKEEGKLQGQLNKSDSNQYIGELKDQI
  AELNHELRCLKGQRGFSGQPPFDGIHIVNHLIGDDESFHSSDEDFIDNSLQETGVGFPLHGKSGSMSLDP
  AVADGSESETEDSVLETRESNQVVQKERPPRRRESYSTTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005665
• ORF Size: 2430 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005665.6
• RefSeq Size: 7403 bp
• RefSeq ORF: 2433 bp
• Locus ID: 7813
• UniProt ID: O60447
• Cytogenetics: 1p22.1
• Domains: TBC
• MW: 92.8 kDa
• Gene Summary: Functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. May play a role in cytokinesis.[UniProtKB/Swiss-Prot Function]