KLHL18 (NM_025010) Human Tagged ORF Clone

CAT#: RC223003

KLHL18 (Myc-DDK-tagged)-Human kelch-like 18 (Drosophila) (KLHL18)

Product Images

Circular map for RC223003

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHL18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223003 representing NM_025010
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGGACGGCGCGGAGGAGCTGGAGGATCTGGTGCACTTCTCCGTGTCTGAGTTGCCTAGTCGCG
  GCTACGGCGTCATGGAGGAGATCCGGCGGCAGGGCAAGCTGTGCGACGTGACCCTCAAGATTGGGGACCA
  CAAATTCAGTGCCCACCGGATTGTCTTAGCAGCCTCGATCCCGTATTTCCATGCTATGTTTACAAATGAC
  ATGATGGAGTGCAAGCAGGATGAGATTGTAATGCAAGGAATGGACCCAAGTGCCCTGGAGGCTCTGATCA
  ACTTTGCCTACAACGGCAACCTTGCCATTGACCAGCAAAATGTCCAGTCATTGCTGATGGGGGCGAGCTT
  CCTGCAGCTGCAGAGCATCAAAGACGCCTGCTGCACATTCCTTCGAGAACGGCTTCACCCAAAAAACTGC
  CTGGGTGTGCGCCAGTTTGCTGAGACAATGATGTGTGCTGTGCTGTACGACGCTGCCAACAGCTTCATCC
  ACCAGCACTTTGTGGAGGTGTCCATGTCAGAAGAGTTCCTGGCCCTGCCCTTGGAAGACGTGCTTGAGCT
  GGTGTCTCGGGATGAGCTGAATGTCAAATCTGAGGAGCAGGTCTTTGAAGCTGCATTGGCCTGGGTCAGA
  TACGACCGGGAGCAGAGGGGTCCCTACCTGCCTGAGCTGCTGTCCAATATCCGCCTGCCCCTCTGTCGGC
  CCCAGTTCCTTTCAGACAGAGTACAGCAGGATGACCTGGTGCGTTGCTGCCACAAATGCAGGGACCTGGT
  AGACGAAGCAAAGGACTACCACCTCATGCCAGAGCGCCGGCCCCACCTGCCAGCTTTCAGAACCCGGCCA
  CGCTGCTGCACATCCATCGCTGGACTTATCTACGCTGTAGGGGGCCTCAACTCAGCAGGTGATTCCCTGA
  ATGTGGTGGAAGTGTTCGACCCCATTGCCAATTGCTGGGAGAGATGCCGTCCCATGACAACAGCCCGCAG
  CCGCGTTGGCGTGGCTGTGGTGAACGGGCTTCTCTATGCCATCGGAGGATATGACGGCCAGCTACGGCTG
  AGCACTGTGGAGGCCTACAACCCGGAGACAGACACATGGACCAGAGTGGGGAGCATGAATAGCAAGAGAA
  GTGCCATGGGGACAGTCGTGCTGGATGGGCAGATCTACGTCTGTGGGGGCTACGATGGCAACTCTTCCCT
  CAGCTCCGTGGAGACCTACTCACCTGAGACGGACAAATGGACAGTGGTGACCTCGATGAGCTCGAATCGC
  AGTGCTGCTGGGGTTACAGTCTTTGAGGGCAGGATATATGTGTCAGGCGGCCATGATGGTTTGCAGATCT
  TCAGCAGTGTGGAACACTACAACCACCACACAGCCACCTGGCACCCTGCAGCTGGCATGCTCAACAAGCG
  CTGCCGGCACGGAGCCGCCTCCCTGGGGAGCAAGATGTTTGTCTGCGGGGGCTACGATGGCTCTGGCTTC
  CTCAGCATTGCCGAGATGTACAGCTCTGTGGCAGACCAGTGGTGCCTGATTGTCCCCATGCACACGCGCA
  GGAGCCGGGTCTCCCTGGTGGCCAGCTGTGGGCGCCTCTACGCTGTTGGGGGCTACGACGGACAGTCAAA
  CCTAAGCTCAGTGGAGATGTATGACCCAGAGACAGACTGCTGGACATTCATGGCCCCCATGGCGTGCCAT
  GAGGGAGGGGTCGGTGTGGGCTGCATCCCTCTCCTCACCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223003 representing NM_025010
  Red=Cloning site Green=Tags(s)
  
  MVEDGAEELEDLVHFSVSELPSRGYGVMEEIRRQGKLCDVTLKIGDHKFSAHRIVLAASIPYFHAMFTND
  MMECKQDEIVMQGMDPSALEALINFAYNGNLAIDQQNVQSLLMGASFLQLQSIKDACCTFLRERLHPKNC
  LGVRQFAETMMCAVLYDAANSFIHQHFVEVSMSEEFLALPLEDVLELVSRDELNVKSEEQVFEAALAWVR
  YDREQRGPYLPELLSNIRLPLCRPQFLSDRVQQDDLVRCCHKCRDLVDEAKDYHLMPERRPHLPAFRTRP
  RCCTSIAGLIYAVGGLNSAGDSLNVVEVFDPIANCWERCRPMTTARSRVGVAVVNGLLYAIGGYDGQLRL
  STVEAYNPETDTWTRVGSMNSKRSAMGTVVLDGQIYVCGGYDGNSSLSSVETYSPETDKWTVVTSMSSNR
  SAAGVTVFEGRIYVSGGHDGLQIFSSVEHYNHHTATWHPAAGMLNKRCRHGAASLGSKMFVCGGYDGSGF
  LSIAEMYSSVADQWCLIVPMHTRRSRVSLVASCGRLYAVGGYDGQSNLSSVEMYDPETDCWTFMAPMACH
  EGGVGVGCIPLLTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025010
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025010.5
• RefSeq Size: 4660 bp
• RefSeq ORF: 1725 bp
• Locus ID: 23276
• UniProt ID: O94889
• Cytogenetics: 3p21.31
• Domains: Kelch
• MW: 63.5 kDa
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis (PubMed:23213400). The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (PubMed:23213400). Regulates light-and dark-dependent alpha-transducin localization changes in rod photoreceptors through UNC119 ubiquitination and degradation (By similarity). Preferentially ubiquitinates the unphosphorylated form of UNC119 over the phosphorylated form (By similarity). In the presence of UNC119, under dark-adapted conditions alpha-transducin mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light (By similarity).[UniProtKB/Swiss-Prot Function]