Syntrophin gamma 2 (SNTG2) (NM_018968) Human Tagged ORF Clone

CAT#: RC222968

SNTG2 (Myc-DDK-tagged)-Human syntrophin, gamma 2 (SNTG2)

Product Images

Circular map for RC222968

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  Western blot validation of overexpression lysate (Cat# LY412855) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222968 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SNTG2 protein (Cat# TP322968). The protein was produced from HEK293T cells transfected with SNTG2 cDNA clone (Cat# RC222968) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syntrophin gamma 2
• Synonyms: G2SYN; SYN5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222968 representing NM_018968
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGAGGGACCCCCGCCCCCGGCCGCCTCCCGCGGACGCCAGGGCTGCCTGCTGGTACCTGCGC
  GGACGAAAACCACTATTGCTCTGTTGTATGATGAAGAGTCCGAAAATGCCTATGACATCCGGCTGAAGCT
  GACGAAAGAGGTGCTGACAATTCAGAAACAAGATGTTGTCTGTGTGGGCGGAAGCCACCAGGGCAGGAAT
  CGCAGAACTGTTACACTCCGCAGACAGCCAGTTGGCGGCTTGGGCCTGAGTATAAAGGGAGGTTCTGAGC
  ACAACGTCCCTGTCGTCATATCAAAAATATTCGAAGACCAAGCAGCTGACCAGACAGGGATGTTGTTCGT
  AGGAGATGCTGTTCTCCAGGTTAATGGCATACATGTAGAAAATGCAACTCATGAAGAAGTGGTGCATCTG
  CTGAGAAATGCTGGCGATGAAGTTACCATCACCGTTGAGTATCTCAGGGAAGCGCCGGCATTTCTGAAGC
  TCCCGTTAGGGTCCCCAGGGCCATCCAGCGACCACAGCAGTGGGGCCTCCTCTCCCCTCTTTGACAGCGG
  TTTGCATCTGAACGGAAACTCCAGTACCACAGCCCCATCGTCACCTTCCTCGCCCATAGCTAAGGACCCG
  AGGTATGAGAAGCGCTGGCTGGACACCTTGTCCGTGCCTCTGTCCATGGCTCGCATCTCAAGGTACAAAG
  CCGGAACGGAAAAATTAAGGTGGAATGCGTTCGAGGTGCTCGCCCTGGACGGAGTCAGCTCTGGGATCCT
  CCGGTTTTACACAGCCCAGGATGGCACCGACTGGCTGCGGGCGGTCTCAGCCAACATCAGGGAGCTGACA
  CTTCAGAACATGAAGATGGCGAACAAATGCTGCTCTCCTTCCGACCAGGTTGTGCATATGGGGTGGGTAA
  ATGAGAAACTCCAAGGAGCTGACTCCTCTCAAACCTTCAGACCCAAGTTCCTAGCACTGAAGGGCCCGTC
  CTTCTACGTTTTCAGCACTCCTCCGGTGAGCACATTCGATTGGGTGCGAGCAGAAAGGACCTATCACCTC
  TGTGAGGTGCTATTTAAAGTTCACAAGTTCTGGCTCACAGAGGACTGCTGGTTGCAAGCAAACTTGTATC
  TGGGTCTTCAAGATTTTGACTTTGAGGACCAGAGGCCCTATTGCTTCAGCATCGTGGCCGGCCATGGGAA
  GAGCCATGTTTTCAACGTGGAGCTTGGCAGCGAGCTGGCCATGTGGGAGAAGTCCTTCCAAAGAGCCACG
  TTCATGGAAGTTCAGAGAACCGGGTCCAGAACATACATGTGCAGCTGGCAAGGAGAGATGCTGTGTTTCA
  CGGTGGATTTCGCGTTGGGATTTACCTGTTTTGAGAGTAAGACCAAGAATGTGCTCTGGAGATTTAAATT
  TTCCCAGCTTAAGGGATCTTCAGATGATGGGAAAACTCGAGTAAAGCTGCTGTTTCAGAATCTGGACACC
  AAACAGATTGAGACGAAGGAACTCGAGTTCCAGGACCTGAGGGCTGTCCTGCACTGCATCCACTCCTTCA
  TAGCAGCCAAGGTGGCCTCCGTGGACCCCGGCTTCATGGACAGTCAGAGTCTTGCCAGAAAATACATGTA
  CAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222968 representing NM_018968
  Red=Cloning site Green=Tags(s)
  
  MGTEGPPPPAASRGRQGCLLVPARTKTTIALLYDEESENAYDIRLKLTKEVLTIQKQDVVCVGGSHQGRN
  RRTVTLRRQPVGGLGLSIKGGSEHNVPVVISKIFEDQAADQTGMLFVGDAVLQVNGIHVENATHEEVVHL
  LRNAGDEVTITVEYLREAPAFLKLPLGSPGPSSDHSSGASSPLFDSGLHLNGNSSTTAPSSPSSPIAKDP
  RYEKRWLDTLSVPLSMARISRYKAGTEKLRWNAFEVLALDGVSSGILRFYTAQDGTDWLRAVSANIRELT
  LQNMKMANKCCSPSDQVVHMGWVNEKLQGADSSQTFRPKFLALKGPSFYVFSTPPVSTFDWVRAERTYHL
  CEVLFKVHKFWLTEDCWLQANLYLGLQDFDFEDQRPYCFSIVAGHGKSHVFNVELGSELAMWEKSFQRAT
  FMEVQRTGSRTYMCSWQGEMLCFTVDFALGFTCFESKTKNVLWRFKFSQLKGSSDDGKTRVKLLFQNLDT
  KQIETKELEFQDLRAVLHCIHSFIAAKVASVDPGFMDSQSLARKYMYSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018968
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018968.4
• RefSeq Size: 1904 bp
• RefSeq ORF: 1620 bp
• Locus ID: 54221
• UniProt ID: Q9NY99
• Cytogenetics: 2p25.3
• MW: 60 kDa
• Gene Summary: This gene encodes a protein belonging to the syntrophin family. Syntrophins are cytoplasmic peripheral membrane proteins that bind to components of mechanosenstive sodium channels and the extreme carboxy-terminal domain of dystrophin and dystrophin-related proteins. The PDZ domain of this protein product interacts with a protein component of a mechanosensitive sodium channel that affects channel gating. Absence or reduction of this protein product has been associated with Duchenne muscular dystrophy. There is evidence of alternative splicing yet the full-length nature of these variants has not been described. [provided by RefSeq, Jul 2008]