Nectin 3 (NECTIN3) (NM_015480) Human Tagged ORF Clone

CAT#: RC222962

PVRL3 (Myc-DDK-tagged)-Human poliovirus receptor-related 3 (PVRL3)

Product Images

Circular map for RC222962

• 

  Western blot validation of overexpression lysate (Cat# LY402442) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222962 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nectin 3
• Synonyms: CD113; CDW113; NECTIN-3; PPR3; PRR3; PVRL3; PVRR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222962 representing NM_015480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGACCCTGCGGCCGTCCCCGCTGTGTCCTGGAGGCGGCAAAGCACAACTTTCCTCCGCTTCTC
  TCCTCGGAGCCGGGCTCCTGCTGCAGCCCCCGACGCCACCTCCGCTGCTGCTGCTGCTCTTCCCGCTGCT
  GCTCTTCTCCAGGCTCTGTGGTGCCTTAGCTGGACCAATTATTGTGGAGCCACATGTCACAGCAGTATGG
  GGAAAGAATGTTTCATTAAAGTGTTTAATTGAAGTAAATGAAACCATAACACAGATTTCATGGGAGAAGA
  TACATGGCAAAAGTTCACAGACTGTTGCAGTTCACCATCCCCAATATGGATTCTCTGTTCAAGGAGAATA
  TCAGGGAAGAGTCTTGTTTAAAAATTACTCACTTAATGATGCAACAATTACTCTGCATAACATAGGATTC
  TCTGATTCTGGAAAATACATCTGCAAAGCTGTTACATTCCCGCTTGGAAATGCCCAGTCCTCTACAACTG
  TAACTGTGTTAGTTGAACCCACTGTGAGCCTGATAAAAGGGCCAGATTCTTTAATTGATGGAGGAAATGA
  AACAGTAGCAGCCATTTGCATCGCAGCCACTGGAAAACCCGTTGCACATATTGACTGGGAAGGTGATCTT
  GGTGAAATGGAATCCACTACAACTTCTTTTCCAAATGAAACGGCAACGATTATCAGCCAGTACAAGCTAT
  TTCCAACCAGATTTGCTAGAGGAAGGCGAATTACTTGTGTTGTAAAACATCCAGCCTTGGAAAAGGACAT
  CCGATACTCTTTCATATTAGACATACAGTATGCTCCTGAAGTTTCGGTAACAGGATATGATGGAAATTGG
  TTTGTAGGAAGAAAAGGTGTTAATCTCAAATGTAATGCTGATGCAAATCCACCACCCTTCAAATCTGTGT
  GGAGCAGGTTGGATGGACAATGGCCTGATGGTTTATTGGCTTCAGACAATACTCTTCATTTTGTCCATCC
  ATTGACTTTCAATTATTCTGGTGTTTATATCTGTAAAGTGACCAATTCCCTTGGTCAAAGAAGTGACCAA
  AAAGTCATCTACATTTCAGATCCTCCTACTACTACCACCCTTCAGCCTACAATTCAGTGGCATCCCTCAA
  CTGCTGACATCGAGGATCTAGCAACAGAACCTAAAAAATTGCCCTTCCCATTGTCAACTTTGGCAACAAT
  TAAGGATGACACAATTGCCACGATCATTGCTAGTGTAGTGGGTGGGGCTCTCTTCATAGTACTTGTAAGT
  GTTTTGGCTGGAATATTCTGCTATAGGAGAAGACGGACGTTTCGTGGAGACTACTTTGCCAAGAACTACA
  TTCCACCATCAGATATGCAAAAAGAATCACAAATAGATGTTCTTCAACAAGATGAGCTTGATTCTTACCC
  AGACAGTGTAAAAAAAGAAAACAAAAATCCAGTGAACAATCTAATACGTAAAGACTATTTAGAAGAGCCT
  GAAAAAACTCAGTGGAACAATGTAGAAAATCTCAATAGGTTTGAAAGACCAATGGATTATTATGAAGATC
  TAAAAATGGGAATGAAGTTTGTCAGTGATGAACATTATGATGAAAACGAAGATGACTTAGTTTCACATGT
  AGATGGTTCCGTAATTTCCAGGAGGGAGTGGTATGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222962 representing NM_015480
  Red=Cloning site Green=Tags(s)
  
  MARTLRPSPLCPGGGKAQLSSASLLGAGLLLQPPTPPPLLLLLFPLLLFSRLCGALAGPIIVEPHVTAVW
  GKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGF
  SDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDL
  GEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNW
  FVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQ
  KVIYISDPPTTTTLQPTIQWHPSTADIEDLATEPKKLPFPLSTLATIKDDTIATIIASVVGGALFIVLVS
  VLAGIFCYRRRRTFRGDYFAKNYIPPSDMQKESQIDVLQQDELDSYPDSVKKENKNPVNNLIRKDYLEEP
  EKTQWNNVENLNRFERPMDYYEDLKMGMKFVSDEHYDENEDDLVSHVDGSVISRREWYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015480
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015480.3
• RefSeq Size: 1650 bp
• RefSeq ORF: 1650 bp
• Locus ID: 25945
• UniProt ID: Q9NQS3
• Cytogenetics: 3q13.13
• Domains: ig, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs)
• MW: 60.8 kDa
• Gene Summary: This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]