SLC7A14 (NM_020949) Human Tagged ORF Clone

CAT#: RC222683

SLC7A14 (Myc-DDK-tagged)-Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 (SLC7A14)

Product Images

Circular map for RC222683

• 

  Western blot validation of overexpression lysate (Cat# LY412174) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222683 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC7A14
• Synonyms: PPP1R142
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222683 representing NM_020949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCTTCTTCACCTCGCTGGACCCCCGGCGGGTGCAGTGGGGAGCTGCCTGGTATGCAATGCACT
  CCAGGATCCTACGCACCAAACCAGTGGAGTCCATGCTAGAGGGAACTGGGACCACCACGGCACATGGAAC
  TAAGCTAGCCCAGGTACTCACCACAGTGGACCTCATCTCTCTTGGCGTTGGCAGCTGTGTGGGCACTGGC
  ATGTATGTGGTCTCTGGCCTGGTGGCCAAGGAAATGGCAGGACCTGGTGTCATTGTGTCCTTCATCATTG
  CAGCCGTCGCATCCATATTATCAGGCGTCTGCTATGCAGAGTTTGGAGTTCGAGTCCCCAAGACCACAGG
  ATCTGCCTACACCTACAGCTATGTCACTGTTGGGGAATTTGTGGCATTTTTCATTGGCTGGAACCTGATC
  CTGGAGTACCTGATTGGCACTGCGGCCGGAGCCAGTGCTCTGAGCAGCATGTTTGACTCACTAGCCAACC
  ACACCATCAGCCGCTGGATGGCGGACAGCGTGGGAACCCTCAATGGCCTGGGGAAAGGTGAAGAATCATA
  CCCAGACCTTCTGGCTCTGTTGATCGCGGTCATCGTGACCATCATTGTTGCTCTGGGGGTGAAGAATTCC
  ATAGGCTTCAACAATGTTCTCAATGTGCTGAACCTGGCAGTATGGGTGTTCATCATGATCGCAGGCCTCT
  TCTTCATCAATGGGAAATACTGGGCGGAGGGCCAGTTCTTGCCCCACGGCTGGTCAGGGGTGCTGCAAGG
  AGCAGCAACATGCTTCTACGCTTTCATTGGCTTTGACATCATCGCCACCACTGGAGAGGAAGCCAAGAAT
  CCCAACACGTCCATCCCTTATGCTATCACTGCCTCCCTGGTCATCTGCCTGACAGCATATGTGTCTGTGA
  GCGTGATCTTAACTCTGATGGTGCCATATTATACCATTGACACGGAATCCCCACTCATGGAGATGTTTGT
  GGCTCATGGGTTCTATGCTGCCAAATTCGTAGTGGCCATTGGGTCGGTTGCAGGACTGACAGTCAGCTTG
  CTGGGGTCCCTCTTCCCGATGCCGAGGGTCATTTATGCCATGGCTGGTGACGGGCTCCTTTTCAGGTTCC
  TGGCTCACGTCAGCTCCTACACAGAGACACCAGTGGTGGCCTGCATCGTGTCGGGGTTCCTGGCAGCGCT
  CCTCGCACTGTTGGTCAGCTTGAGAGACCTGATAGAGATGATGTCTATCGGCACGCTCCTGGCCTACACC
  TTGGTCTCTGTCTGTGTCTTGCTCCTTCGATACCAACCTGAGAGTGACATTGATGGTTTTGTCAAGTTCT
  TGTCTGAGGAGCACACCAAGAAGAAGGAGGGCATTCTGGCTGACTGTGAGAAGGAAGCTTGTTCTCCTGT
  GAGTGAGGGGGATGAGTTTTCTGGCCCAGCCACCAACACATGTGGGGCCAAGAACTTACCATCCTTGGGA
  GACAATGAGATGCTCATAGGGAGATCAGACAAGTCAACCTACAACGTCAACCACCCCAATTACGGCACCG
  TGGACATGACCACAGGCATAGAAGCTGATGAATCCGAAAATATTTATCTCATCAAGTTAAAGAAGCTGAT
  TGGGCCTCATTATTACACCATGAGAATCCGGCTGGGCCTTCCAGGCAAAATGGACCGGCCCACAGCAGCG
  ACGGGGCACACGGTGACCATCTGCGTGCTCCTGCTCTTCATCCTCATGTTCATCTTCTGCTCCTTCATCA
  TCTTTGGTTCTGACTACATCTCAGAGCAGAGCTGGTGGGCCATCCTTCTGGTTGTTCTGATGGTGCTGCT
  GATCAGCACCCTGGTGTTTGTGATCCTGCAGCAGCCAGAGAACCCCAAGAAGCTGCCCTACATGGCCCCT
  TGCCTCCCCTTTGTGCCTGCCTTTGCCATGCTGGTGAACATCTATCTCATGCTAAAGCTCTCCACCATCA
  CATGGATCCGGTTTGCGGTCTGGTGCTTTGTGGGTCTGCTCATTTATTTTGGATATGGCATCTGGAACAG
  CACCCTGGAAATCAGCGCTCGAGAAGAGGCCCTGCACCAAAGCACGTACCAACGCTACGACGTGGATGAC
  CCCTTCTCAGTGGAGGAGGGTTTCTCCTACGCCACAGAGGGCGAGAGCCAGGAGGACTGGGGCGGGCCCA
  CTGAAGACAAAGGCTTCTATTACCAACAGATGTCAGATGCGAAGGCAAACGGCCGGACAAGTAGCAAAGC
  GAAGAGCAAAAGCAAACACAAACAGAACTCAGAGGCCCTGATTGCAAATGATGAGTTAGATTACTCTCCA
  GAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222683 representing NM_020949
  Red=Cloning site Green=Tags(s)
  
  MSGFFTSLDPRRVQWGAAWYAMHSRILRTKPVESMLEGTGTTTAHGTKLAQVLTTVDLISLGVGSCVGTG
  MYVVSGLVAKEMAGPGVIVSFIIAAVASILSGVCYAEFGVRVPKTTGSAYTYSYVTVGEFVAFFIGWNLI
  LEYLIGTAAGASALSSMFDSLANHTISRWMADSVGTLNGLGKGEESYPDLLALLIAVIVTIIVALGVKNS
  IGFNNVLNVLNLAVWVFIMIAGLFFINGKYWAEGQFLPHGWSGVLQGAATCFYAFIGFDIIATTGEEAKN
  PNTSIPYAITASLVICLTAYVSVSVILTLMVPYYTIDTESPLMEMFVAHGFYAAKFVVAIGSVAGLTVSL
  LGSLFPMPRVIYAMAGDGLLFRFLAHVSSYTETPVVACIVSGFLAALLALLVSLRDLIEMMSIGTLLAYT
  LVSVCVLLLRYQPESDIDGFVKFLSEEHTKKKEGILADCEKEACSPVSEGDEFSGPATNTCGAKNLPSLG
  DNEMLIGRSDKSTYNVNHPNYGTVDMTTGIEADESENIYLIKLKKLIGPHYYTMRIRLGLPGKMDRPTAA
  TGHTVTICVLLLFILMFIFCSFIIFGSDYISEQSWWAILLVVLMVLLISTLVFVILQQPENPKKLPYMAP
  CLPFVPAFAMLVNIYLMLKLSTITWIRFAVWCFVGLLIYFGYGIWNSTLEISAREEALHQSTYQRYDVDD
  PFSVEEGFSYATEGESQEDWGGPTEDKGFYYQQMSDAKANGRTSSKAKSKSKHKQNSEALIANDELDYSP
  E
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020949
• ORF Size: 2313 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020949.1, NP_066000.1
• RefSeq Size: 2909 bp
• RefSeq ORF: 2316 bp
• Locus ID: 57709
• UniProt ID: Q8TBB6
• Cytogenetics: 3q26.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 83.9 kDa
• Gene Summary: This gene is predicted to encode a glycosylated, cationic amino acid transporter protein with 14 transmembrane domains. This gene is primarily expressed in skin fibroblasts, neural tissue, and primary endothelial cells and its protein is predicted to mediate lysosomal uptake of cationic amino acids. Mutations in this gene are associated with autosomal recessive retinitis pigmentosa. In mice, this gene is expressed in the photoreceptor layer of the retina where its expression increases over the course of retinal development and persists in the mature retina. [provided by RefSeq, Apr 2014]