STRC (NM_153700) Human Tagged ORF Clone

CAT#: RC222482

STRC (Myc-DDK-tagged)-Human stereocilin (STRC)

Product Images

Circular map for RC222482

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STRC
• Synonyms: DFNB16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222482 representing NM_153700
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCAGCCTCTGGCCCCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGTCCTTTGCAGTGACTC
  TGGCCCCTACTGGGCCTCATTCCCTGGACCCTGGTCTCTCCTTCCTGAAGTCATTGCTCTCCACTCTGGA
  CCAGGCTCCCCAGGGCTCCCTGAGCCGCTCACGGTTCTTTACATTCCTGGCCAACATTTCTTCTTCCTTT
  GAGCCTGGGAGAATGGGGGAAGGACCAGTAGGAGAGCCCCCACCTCTCCAGCCGCCTGCTCTGCGGCTCC
  ATGATTTTCTAGTGACACTGAGAGGTAGCCCCGACTGGGAGCCAATGCTAGGGCTGCTAGGGGATATGCT
  GGCACTGCTGGGACAGGAGCAGACTCCCCGAGATTTCCTGGTGCACCAGGCAGGGGTGCTGGGTGGACTT
  GTGGAGGTGCTGCTGGGAGCCTTAGTTCCTGGGGGCCCCCCTACCCCAACTCGGCCCCCATGCACCCGTG
  ATGGGCCGTCTGACTGTGTCCTGGCTGCTGACTGGTTGCCTTCTCTGCTGCTGTTGTTAGAGGGCACACG
  CTGGCAAGCTCTGGTGCAGGTGCAGCCCAGTGTGGACCCCACCAATGCCACAGGCCTCGATGGGAGGGAG
  GCAGCTCCTCACTTTTTGCAGGGTCTGTTGGGTTTGCTTACCCCAACAGGGGAGCTAGGCTCCAAGGAGG
  CTCTTTGGGGCGGTCTGCTACGCACAGTGGGGGCCCCCCTCTATGCTGCCTTTCAGGAGGGGCTGCTCCG
  TGTCACTCACTCCCTGCAGGATGAGGTCTTCTCCATTTTGGGGCAGCCAGAGCCTGATACCAATGGGCAG
  TGCCAGGGAGGTAACCTTCAACAGCTGCTCTTATGGGGCGTCCGGCACAACCTTTCCTGGGATGTCCAGG
  CGCTGGGCTTTCTGTCTGGATCACCACCCCCACCCCCTGCCCTCCTTCACTGCCTGAGCACGGGCGTGCC
  TCTGCCCAGAGCTTCTCAGCCGTCAGCCCACATCAGCCCACGCCAACGGCGAGCCATCACTGTGGAGGCC
  CTCTGTGAGAACCACTTAGGCCCAGCACCACCCTACAGCATTTCCAACTTCTCCATCCACTTGCTCTGCC
  AGCACACCAAGCCTGCCACTCCACAGCCCCATCCCAGCACCACTGCCATCTGCCAGACAGCTGTGTGGTA
  TGCAGTGTCCTGGGCACCAGGTGCCCAAGGCTGGCTACAGGCCTGCCACGACCAGTTTCCTGATGAGTTT
  TTGGATGCGATCTGCAGTAACCTCTCCTTTTCAGCCCTGTCTGGCTCCAACCGCCGCCTGGTGAAGCGGC
  TCTGTGCTGGCCTGCTCCCACCCCCTACCAGCTGCCCTGAAGGCCTGCCCCCTGTTCCCCTCACCCCAGA
  CATCTTTTGGGGCTGCTTCTTGGAGAATGAGACTCTGTGGGCTGAGCGACTGTGTGGGGAGGCAAGTCTA
  CAGGCTGTGCCCCCCAGCAACCAGGCTTGGGTCCAGCATGTGTGCCAGGGCCCCACCCCAGATGTCACTG
  CCTCCCCACCATGCCACATTGGACCCTGTGGGGAACGCTGCCCGGATGGGGGCAGCTTCCTGGTGATGGT
  CTGTGCCAATGACACCATGTATGAGGTCCTGGTGCCCTTCTGGCCTTGGCTAGCAGGCCAATGCAGGATA
  AGTCGTGGGGGCAATGACACTTGCTTCCTAGAAGGGCTGCTGGGCCCCCTTCTGCCCTCTCTGCCACCAC
  TGGGACCATCCCCACTCTGTCTGACCCCTGGCCCCTTCCTCCTTGGCATGCTATCCCAGTTGCCACGCTG
  TCAGTCCTCTGTCCCAGCTCTTGCTCACCCCACACGCCTACACTATCTCCTCCGCCTGCTGACCTTCCTC
  TTGGGTCCAGGGGCTGGGGGCGCTGAGGCCCAGGGGATGCTGGGTCGGGCCCTACTGCTCTCCAGTCTCC
  CAGACAACTGCTCCTTCTGGGATGCCTTTCGCCCAGAGGGCCGGCGCAGTGTGCTACGGACGATTGGGGA
  ATACCTGGAACAAGATGAGGAGCAGCCAACCCCATCAGGCTTTGAACCCACTGTCAACCCCAGCTCTGGT
  ATAAGCAAGATGGAGCTGCTGGCCTGCTTTAGTCCTGTGCTGTGGGATCTGCTCCAGAGGGAAAAGAGTG
  TTTGGGCCCTGCAGATTCTAGTGCAGGCGTACCTGCATATGCCCCCAGAAAACCTCCAGCAGCTGGTGCT
  TTCAGCAGAGAGGGAGGCTGCACAGGGCTTCCTGACACTCATGCTGCAGGGGAAGCTGCAGGGGAAGCTG
  CAGGTACCACCATCCGAGGAGCAGGCCCTGGGTCGCCTGACAGCCCTGCTGCTCCAGCGGTACCCACGCC
  TCACCTCCCAGCTCTTCATTGACCTGTCACCACTCATCCCTTTCTTGGCTGTCTCTGACCTGATGCGCTT
  CCCACCATCCCTGTTAGCCAACGACAGTGTCCTGGCTGCCATCCGGGATTACAGCCCAGGAATGAGGCCT
  GAACAGAAGGAGGCTCTGGCAAAGCGACTGCTGGCCCCTGAACTGTTTGGGGAAGTGCCTGCCTGGCCCC
  AGGAGCTGCTGTGGGCAGTGCTGCCCCTGCTCCCCCACCTCCCTCTGGAGAACTTTTTGCAGCTCAGCCC
  TCACCAGATCCAGGCCCTGGAGGATAGCTGGCCAGCAGCAGGTCTGGGGCCAGGGCATGCCCGCCATGTG
  CTGCGCAGCCTGGTAAACCAGAGTGTCCAGGATGGTGAGGAGCAGGTACGCAGGCTTGGGCCCCTCGCCT
  GTTTCCTGAGCCCTGAGGAGCTGCAGAGCCTAGTGCCCCTGAGTGATCCAACGGGGCCAGTAGAACGGGG
  GCTGCTGGAATGTGCAGCCAATGGGACCCTCAGCCCAGAAGGACGGGTGGCATATGAACTTCTGGGTGTG
  TTGCGCTCATCTGGAGGAGCGGTGCTGAGCCCCCGGGAGCTGCGGGTCTGGGCCCCTCTCTTCTCTCAGC
  TGGGCCTCCGCTTCCTTCAGGAGCTGTCAGAGCCCCAGCTTAGAGCCATGCTTCCTGTCCTGCAGGGAAC
  TAGTGTTACACCTGCTCAGGCTGTCCTGCTGCTTGGACGGCTCCTTCCTAGGCACGATCTATCCCTGGAG
  GAACTCTGCTCCTTGCACCTTCTGCTACCAGGCCTCAGCCCCCAGACACTCCAGGCCATCCCTAGGCGAG
  TCCTGGTCGGGGCTTGTTCCTGCCTGGCCCCTGAACTGTCACGCCTCTCAGCCTGCCAGACCGCAGCACT
  GCTGCAGACCTTTCGGGTTAAAGATGGTGTTAAAAATATGGGTACAACAGGTGCTGGTCCAGCTGTGTGT
  ATCCCTGGTCAGCCTATTCCCACCACCTGGCCAGACTGCCTGCTTCCCCTGCTCCCATTAAAGCTGCTAC
  AACTGGATTCCTTGGCTCTTCTGGCAAATCGAAGACGCTACTGGGAGCTGCCCTGGTCTGAGCAGCAGGC
  ACAGTTTCTCTGGAAGAAGATGCAAGTACCCACCAACCTTACCCTCAGGAATCTGCAGGCTCTGGGCACC
  CTGGCAGGAGGCATGTCCTGTGAGTTTCTGCAGCAGATCAACTCCATGGTAGACTTCCTTGAAGTGGTGC
  ACATGATCTATCAGCTGCCCACTAGAGTTCGAGGGAGCCTGAGGGCCTGTATCTGGGCAGAGCTACAGCG
  GAGGATGGCAATGCCAGAACCAGAATGGACAACTGTAGGGCCAGAACTGAACGGGCTGGATAGCAAGCTA
  CTCCTGGACTTACCGATCCAGTTGATGGACAGACTATCCAATGAATCCATTATGTTGGTGGTGGAGCTGG
  TGCAAAGAGCTCCAGAGCAGCTGCTGGCACTGACCCCCCTCCACCAGGCAGCCCTGGCAGAGAGGGCACT
  ACAAAACCTGGCTCCAAAGGAGACTCCAGTCTCAGGGGAAGTGCTGGAGACCTTAGGCCCTTTGGTTGGA
  TTCCTGGGGACAGAGAGCACACGACAGATCCCCCTACAGATCCTGCTGTCCCATCTCAGTCAGCTGCAAG
  GCTTCTGCCTAGGAGAGACATTTGCCACAGAGCTGGGATGGCTGCTATTGCAGGAGTCTGTTCTTGGGAA
  ACCAGAGTTGTGGAGCCAGGATGAAGTAGAGCAAGCTGGACGCCTAGTATTCACTCTGTCTACTGAGGCA
  ATTTCCTTGATCCCCAGGGAGGCCTTGGGTCCAGAGACCCTGGAGCGGCTTCTAGAAAAGCAGCAGAGCT
  GGGAGCAGAGCAGAGTTGGACAGCTGTGTAGGGAGCCACAGCTTGCTGCCAAGAAAGCAGCCCTGGTAGC
  AGGGGTGGTGCGACCAGCTGCTGAGGATCTTCCAGAACCTGTGCCAAATTGTGCAGATGTACGAGGGACA
  TTCCCAGCAGCCTGGTCTGCAACCCAGATTGCAGAGATGGAGCTCTCAGACTTTGAGGACTGCCTGACAT
  TATTTGCAGGAGACCCAGGACTTGGGCCTGAGGAACTGCGGGCAGCCATGGGCAAAGCAAAACAGTTGTG
  GGGTCCCCCCCGGGGATTTCGTCCTGAGCAGATCCTGCAGCTTGGTAGGCTCTTAATAGGTCTAGGAGAT
  CGGGAACTACAGGAGCTGATCCTAGTGGACTGGGGAGTGCTGAGCACCCTGGGGCAGATAGATGGCTGGA
  GCACCACTCAGCTCCGCATTGTGGTCTCCAGTTTCCTACGGCAGAGTGGTCGGCATGTGAGCCACCTGGA
  CTTCGTTCATCTGACAGCGCTGGGTTATACTCTCTGTGGACTGCGGCCAGAGGAGCTCCAGCACATCAGC
  AGTTGGGAGTTCAGCCAAGCAGCTCTCTTCCTCGGCACCCTGCATCTCCAGTGCTCTGAGGAACAACTGG
  AGGTTCTGGCCCACCTACTTGTACTGCCTGGTGGGTTTGGCCCAATCAGTAACTGGGGGCCTGAGATCTT
  CACTGAAATTGGCACCATAGCAGCTGGGATCCCAGACCTGGCTCTTTCAGCACTGCTGCGGGGACAGATC
  CAGGGCGTTACTCCTCTTGCCATTTCTGTCATCCCTCCTCCTAAATTTGCTGTGGTGTTTAGTCCCATCC
  AACTATCTAGTCTCACCAGTGCTCAGGCTGTGGCTGTCACTCCTGAGCAAATGGCCTTTCTGAGTCCTGA
  GCAGCGACGAGCAGTTGCATGGGCCCAACATGAGGGAAAGGAGAGCCCAGAACAGCAAGGTCGAAGTACA
  GCCTGGGGCCTCCAGGACTGGTCACGACCTTCCTGGTCCCTGGTATTGACTATCAGCTTCCTTGGCCACC
  TGCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222482 representing NM_153700
  Red=Cloning site Green=Tags(s)
  
  MALSLWPLLLLLLLLLLLSFAVTLAPTGPHSLDPGLSFLKSLLSTLDQAPQGSLSRSRFFTFLANISSSF
  EPGRMGEGPVGEPPPLQPPALRLHDFLVTLRGSPDWEPMLGLLGDMLALLGQEQTPRDFLVHQAGVLGGL
  VEVLLGALVPGGPPTPTRPPCTRDGPSDCVLAADWLPSLLLLLEGTRWQALVQVQPSVDPTNATGLDGRE
  AAPHFLQGLLGLLTPTGELGSKEALWGGLLRTVGAPLYAAFQEGLLRVTHSLQDEVFSILGQPEPDTNGQ
  CQGGNLQQLLLWGVRHNLSWDVQALGFLSGSPPPPPALLHCLSTGVPLPRASQPSAHISPRQRRAITVEA
  LCENHLGPAPPYSISNFSIHLLCQHTKPATPQPHPSTTAICQTAVWYAVSWAPGAQGWLQACHDQFPDEF
  LDAICSNLSFSALSGSNRRLVKRLCAGLLPPPTSCPEGLPPVPLTPDIFWGCFLENETLWAERLCGEASL
  QAVPPSNQAWVQHVCQGPTPDVTASPPCHIGPCGERCPDGGSFLVMVCANDTMYEVLVPFWPWLAGQCRI
  SRGGNDTCFLEGLLGPLLPSLPPLGPSPLCLTPGPFLLGMLSQLPRCQSSVPALAHPTRLHYLLRLLTFL
  LGPGAGGAEAQGMLGRALLLSSLPDNCSFWDAFRPEGRRSVLRTIGEYLEQDEEQPTPSGFEPTVNPSSG
  ISKMELLACFSPVLWDLLQREKSVWALQILVQAYLHMPPENLQQLVLSAEREAAQGFLTLMLQGKLQGKL
  QVPPSEEQALGRLTALLLQRYPRLTSQLFIDLSPLIPFLAVSDLMRFPPSLLANDSVLAAIRDYSPGMRP
  EQKEALAKRLLAPELFGEVPAWPQELLWAVLPLLPHLPLENFLQLSPHQIQALEDSWPAAGLGPGHARHV
  LRSLVNQSVQDGEEQVRRLGPLACFLSPEELQSLVPLSDPTGPVERGLLECAANGTLSPEGRVAYELLGV
  LRSSGGAVLSPRELRVWAPLFSQLGLRFLQELSEPQLRAMLPVLQGTSVTPAQAVLLLGRLLPRHDLSLE
  ELCSLHLLLPGLSPQTLQAIPRRVLVGACSCLAPELSRLSACQTAALLQTFRVKDGVKNMGTTGAGPAVC
  IPGQPIPTTWPDCLLPLLPLKLLQLDSLALLANRRRYWELPWSEQQAQFLWKKMQVPTNLTLRNLQALGT
  LAGGMSCEFLQQINSMVDFLEVVHMIYQLPTRVRGSLRACIWAELQRRMAMPEPEWTTVGPELNGLDSKL
  LLDLPIQLMDRLSNESIMLVVELVQRAPEQLLALTPLHQAALAERALQNLAPKETPVSGEVLETLGPLVG
  FLGTESTRQIPLQILLSHLSQLQGFCLGETFATELGWLLLQESVLGKPELWSQDEVEQAGRLVFTLSTEA
  ISLIPREALGPETLERLLEKQQSWEQSRVGQLCREPQLAAKKAALVAGVVRPAAEDLPEPVPNCADVRGT
  FPAAWSATQIAEMELSDFEDCLTLFAGDPGLGPEELRAAMGKAKQLWGPPRGFRPEQILQLGRLLIGLGD
  RELQELILVDWGVLSTLGQIDGWSTTQLRIVVSSFLRQSGRHVSHLDFVHLTALGYTLCGLRPEELQHIS
  SWEFSQAALFLGTLHLQCSEEQLEVLAHLLVLPGGFGPISNWGPEIFTEIGTIAAGIPDLALSALLRGQI
  QGVTPLAISVIPPPKFAVVFSPIQLSSLTSAQAVAVTPEQMAFLSPEQRRAVAWAQHEGKESPEQQGRST
  AWGLQDWSRPSWSLVLTISFLGHLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153700
• ORF Size: 5325 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153700.2, NP_714544.1
• RefSeq Size: 5515 bp
• RefSeq ORF: 5328 bp
• Locus ID: 161497
• UniProt ID: Q7RTU9
• Cytogenetics: 15q15.3
• Protein Families: Transmembrane
• MW: 192.8 kDa
• Gene Summary: This gene encodes a protein that is associated with the hair bundle of the sensory hair cells in the inner ear. The hair bundle is composed of stiff microvilli called stereocilia and is involved with mechanoreception of sound waves. This gene is part of a tandem duplication on chromosome 15; the second copy is a pseudogene. Mutations in this gene cause autosomal recessive non-syndromic deafness. [provided by RefSeq, Jul 2008]