ADCY2 (NM_020546) Human Tagged ORF Clone

CAT#: RC222444

ADCY2 (Myc-DDK-tagged)-Human adenylate cyclase 2 (brain) (ADCY2)

Product Images

Circular map for RC222444

• 

  Western blot validation of overexpression lysate (Cat# LY412417) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222444 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCY2
• Synonyms: AC2; HBAC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222444 representing NM_020546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCAGGAGGCGATGCGGCGCCGCCGCTACCTGCGGGACCGCTCCGAGGAGGCGGCGGGCGGCGGAG
  ACGGGCTGCCGCGGTCCCGGGACTGGCTCTACGAGTCCTACTACTGCATGAGCCAGCAGCACCCGCTCAT
  CGTCTTCCTGCTGCTCATCGTCATGGGCTCCTGCCTCGCCCTGCTCGCCGTCTTCTTCGCGCTCGGGCTG
  GAAGTTGAAGACCATGTGGCGTTTCTAATAACAGTTCCAACTGCCCTGGCGATTTTCTTTGCGATATTTA
  TCCTGGTCTGCATCGAGTCTGTGTTTAAGAAGCTGCTGCGCCTCTTCTCGTTGGTGATATGGATATGCCT
  TGTTGCCATGGGATACCTGTTCATGTGTTTTGGAGGCACCGTCTCTCCCTGGGACCAGGTATCGTTCTTC
  CTCTTCATCATCTTCGTGTTGTACACCATGCTGCCCTTCAACATGCGAGACGCCATCATTGCCAGCGTCC
  TCACCTCCTCCTCCCACACCATCGTGCTTAGCGTCTGCCTGTCTGCAACACCGGGAGGCAAGGAGCACCT
  GGTCTGGCAGATCCTGGCCAATGTGATCATTTTCATCTGTGGGAACCTGGCGGGAGCCTACCATAAGCAC
  CTCATGGAACTCGCTCTTCAGCAAACATATCAGGACACCTGTAATTGCATCAAGTCGCGGATCAAGTTGG
  AATTTGAAAAACGTCAACAGGAGCGGCTTCTGCTCTCCCTGCTGCCGGCCCACATCGCCATGGAGATGAA
  AGCGGAGATCATCCAGAGGCTGCAGGGCCCCAAGGCGGGCCAGATGGAGAACACAAATAACTTCCACAAC
  CTGTATGTGAAGCGGCATACAAACGTGAGCATCTTATACGCTGACATCGTTGGCTTTACCCGGCTGGCAA
  GTGACTGCTCCCCGGGAGAACTAGTCCACATGCTGAATGAGCTCTTTGGAAAGTTTGATCAAATTGCAAA
  GGAGAATGAATGCATGAGAATTAAAATTTTAGGAGACTGCTACTACTGTGTATCTGGACTCCCTATATCT
  CTCCCTAACCATGCCAAGAACTGTGTGAAAATGGGGCTGGACATGTGTGAAGCCATAAAGAAAGTGAGGG
  ATGCTACTGGAGTTGATATCAACATGCGCGTGGGCGTGCATTCTGGGAATGTCCTGTGTGGCGTGATTGG
  TCTGCAGAAGTGGCAATATGATGTGTGGTCACATGATGTGACCTTGGCCAACCACATGGAAGCTGGAGGG
  GTCCCTGGACGTGTTCACATTTCTTCTGTCACCCTGGAGCACTTGAATGGCGCTTATAAAGTGGAGGAGG
  GAGATGGTGACATTAGGGACCCATATTTAAAACAGCACCTGGTGAAAACCTACTTTGTGATCAACCCCAA
  GGGAGAACGACGGAGCCCCCAGCATCTCTTCAGACCTCGCCACACCCTTGATGGAGCCAAAATGAGGGCC
  TCGGTCCGCATGACCCGGTACTTGGAGTCCTGGGGGGCAGCCAAGCCCTTTGCACACCTACATCACAGGG
  ACAGCATGACCACAGAGAACGGCAAGATCAGCACCACGGATGTACCCATGGGTCAGCATAATTTTCAAAA
  TCGCACCTTAAGAACCAAGTCACAAAAGAAGAGATTTGAAGAAGAATTGAATGAAAGGATGATTCAAGCA
  ATTGATGGGATTAATGCACAGAAGCAATGGCTCAAGTCTGAAGACATTCAGAGAATCTCACTGCTTTTCT
  ATAACAAAGTACTAGAAAAAGAGTACCGGGCCACGGCACTGCCAGCGTTCAAGTATTATGTGACTTGTGC
  CTGTCTCATATTCTTCTGCATCTTCATTGTGCAGATTCTCGTGCTGCCAAAAACGTCTGTCCTGGGCATC
  TCCTTTGGGGCTGCGTTTCTCTTGCTGGCCTTCATCCTCTTCGTCTGCTTTGCTGGACAGCTTCTGCAAT
  GCAGCAAAAAAGCCTCTCCCCTGCTCATGTGGCTTTTGAAGTCCTCGGGCATCATTGCCAACCGCCCCTG
  GCCACGGATCTCTCTCACGATCATCACCACAGCCATCATATTAATGATGGCCGTGTTCAACATGTTTTTC
  CTGAGTGACTCAGAGGAAACAATCCCTCCAACTGCCAACACAACAAACACAAGCTTTTCAGCCTCAAATA
  ATCAGGTGGCGATTCTGCGTGCGCAGAATTTATTTTTCCTCCCGTACTTTATCTACAGCTGCATTCTGGG
  ACTGATATCCTGTTCCGTGTTCCTGCGGGTAAACTATGAGCTGAAGATGTTGATCATGATGGTGGCCTTG
  GTGGGCTACAACACCATCCTACTCCACACCCACGCCCACGTCCTGGGCGACTACAGCCAGGTCTTATTTG
  AGAGACCAGGCATTTGGAAAGACCTGAAGACCATGGGCTCTGTGTCTCTCTCTATATTCTTCATCACACT
  GCTTGTTCTGGGTAGACAGAATGAATATTACTGTAGGTTAGACTTCTTATGGAAGAACAAATTCAAAAAA
  GAGCGGGAGGAGATAGAGACCATGGAGAACCTGAACCGCGTGCTGCTGGAGAACGTGCTTCCCGCGCACG
  TGGCTGAGCACTTCCTGGCCAGGAGCCTGAAGAATGAGGAGCTATACCACCAGTCCTATGACTGCGTCTG
  TGTCATGTTTGCCTCCATTCCGGATTTCAAAGAATTTTATACAGAATCCGACGTGAACAAGGAGGGCTTG
  GAATGCCTTCGGCTCCTGAACGAGATCATCGCTGACTTTGATGATCTTCTTTCCAAGCCAAAATTCAGTG
  GAGTTGAAAAGATTAAGACCATTGGCAGCACATACATGGCAGCAACAGGTCTGAGCGCTGTGCCCAGCCA
  GGAGCACTCCCAGGAGCCCGAGCGGCAGTACATGCACATTGGCACCATGGTGGAGTTTGCTTTTGCCCTG
  GTAGGGAAGCTGGATGCCATCAACAAGCACTCCTTCAACGACTTCAAATTGCGAGTGGGTATTAACCATG
  GACCTGTGATAGCTGGTGTGATTGGAGCTCAGAAGCCACAATATGATATCTGGGGCAACACTGTCAATGT
  GGCCAGTAGGATGGACAGCACCGGAGTCCTGGACAAAATACAGGTTACCGAGGAGACGAGCCTCGTCCTG
  CAGACCCTCGGATACACGTGCACCTGTCGAGGAATAATCAACGTGAAAGGAAAGGGGGACCTGAAGACGT
  ACTTTGTAAACACAGAAATGTCAAGGTCCCTTTCCCAGAGCAACGTGGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222444 representing NM_020546
  Red=Cloning site Green=Tags(s)
  
  MWQEAMRRRRYLRDRSEEAAGGGDGLPRSRDWLYESYYCMSQQHPLIVFLLLIVMGSCLALLAVFFALGL
  EVEDHVAFLITVPTALAIFFAIFILVCIESVFKKLLRLFSLVIWICLVAMGYLFMCFGGTVSPWDQVSFF
  LFIIFVLYTMLPFNMRDAIIASVLTSSSHTIVLSVCLSATPGGKEHLVWQILANVIIFICGNLAGAYHKH
  LMELALQQTYQDTCNCIKSRIKLEFEKRQQERLLLSLLPAHIAMEMKAEIIQRLQGPKAGQMENTNNFHN
  LYVKRHTNVSILYADIVGFTRLASDCSPGELVHMLNELFGKFDQIAKENECMRIKILGDCYYCVSGLPIS
  LPNHAKNCVKMGLDMCEAIKKVRDATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGG
  VPGRVHISSVTLEHLNGAYKVEEGDGDIRDPYLKQHLVKTYFVINPKGERRSPQHLFRPRHTLDGAKMRA
  SVRMTRYLESWGAAKPFAHLHHRDSMTTENGKISTTDVPMGQHNFQNRTLRTKSQKKRFEEELNERMIQA
  IDGINAQKQWLKSEDIQRISLLFYNKVLEKEYRATALPAFKYYVTCACLIFFCIFIVQILVLPKTSVLGI
  SFGAAFLLLAFILFVCFAGQLLQCSKKASPLLMWLLKSSGIIANRPWPRISLTIITTAIILMMAVFNMFF
  LSDSEETIPPTANTTNTSFSASNNQVAILRAQNLFFLPYFIYSCILGLISCSVFLRVNYELKMLIMMVAL
  VGYNTILLHTHAHVLGDYSQVLFERPGIWKDLKTMGSVSLSIFFITLLVLGRQNEYYCRLDFLWKNKFKK
  EREEIETMENLNRVLLENVLPAHVAEHFLARSLKNEELYHQSYDCVCVMFASIPDFKEFYTESDVNKEGL
  ECLRLLNEIIADFDDLLSKPKFSGVEKIKTIGSTYMAATGLSAVPSQEHSQEPERQYMHIGTMVEFAFAL
  VGKLDAINKHSFNDFKLRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMDSTGVLDKIQVTEETSLVL
  QTLGYTCTCRGIINVKGKGDLKTYFVNTEMSRSLSQSNVAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020546
• ORF Size: 3273 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020546.2, NP_065433.2
• RefSeq Size: 6553 bp
• RefSeq ORF: 3276 bp
• Locus ID: 108
• UniProt ID: Q08462
• Cytogenetics: 5p15.31
• Domains: CYCc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
• MW: 123.4 kDa
• Gene Summary: This gene encodes a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This enzyme is insensitive to Ca(2+)/calmodulin, and is stimulated by the G protein beta and gamma subunit complex. [provided by RefSeq, Jul 2008]