AGAP6 (NM_001077665) Human Tagged ORF Clone

CAT#: RC222390

AGAP6 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 6 (AGAP6)

Product Images

Circular map for RC222390

• 

  Western blot validation of overexpression lysate (Cat# LY421474) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222390 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AGAP6
• Synonyms: AGAP-6; CTGLF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222390 representing NM_001077665
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACATACTGACCTGTCGTGTGCACCCTAGCGTCAGCCTCGAGTTTGACCAGCAGCAGGGGTCGG
  TGTGTCCCTCTGAATCTGAGACCTATGAGGCAGGAGCTAGGGACAGGATGGCAGGAGCGCCCATGGCTGC
  TGCTGTACAGCCTGCTGAGGTGACTGTTGAAGTTGGTGAGGACCTCCACATGCACCACGTTCGTGACCGG
  GAGATGCCTGAAGCTTTGGAGTTTAACCTTTCTGCCAATCCAGAGTCAAGCACAATATTCCAGAGGAACT
  CTCAAACAGAAGCTTTGGAGTTTAACCCTTCTGCCAATCCAGAGGCAAGCACAATATTCCAGAGGAACTC
  TCAAACAGATGTTGTAGAAATAAGAAGAAGCAACTGTACAAACCATGTATCTGCTGTGCGTTTCAGTCAA
  CAATACAGCTTGTGTTCGACAATATTCCTTGATGACAGCACAGCCATCCAGCATTATCTTACAATGACAA
  TAATATCTGTGACCTTGGAGATACCTCATCATATCACACAAAGAGATGCAGATAGAACTTTGAGCATACC
  TGATGAACAGTTACACTCATTTGCGGTTTCCACCGTGCACATTATGAAGAAAAGAAATGGAGGTGGGAGT
  TTAAATAACTATTCCTCCTCCATTCCATCGACTCCCAGCACCAGCCAGGAGGACCCTCAGTTCAGTGTTC
  CTCCCACTGCCAACACACCCACGCCCGTTTGCAAGCGGTCCATGCGCTGGTCCAACCTGTTTACATCTGA
  GAAAGGGAGTGACCCAGACAAAGAGAGGAAAGCCCCGGAGAATCATGCTGACACCATCGGGAGCGGTAGA
  GCCATCCCCATTAAACAGGGCATGCTCTTAAAGCGAAGTGGGAAATGGCTGAAGACATGGAAAAAGAAAT
  ACGTCACCCTGTGTTCCAATGGCATGCTCACCTATTATTCAAGCTTAGGTGATTATATGAAGAATATTCA
  TAAAAAAGAGATTGACCTTCAGACATCTACCATCAAAGTCCCAGGAAAGTGGCCATCCCTAGCCACATCG
  GCCTGCACACCCATCTCCAGCTCTAAAAGCAATGGCCTATCCAAGGACATGGACACCGGGCTGGGTGACT
  CCATATGCTTCAGCCCCAGTATCTCCAGCACCACCAGCCCCAAGCTCAACCCGCCCCCCTCTCCTCATGC
  TAATAAAAAGAAACACCTAAAGAAGAAAAGCACCAACAACTTTATGATTGTGTCTGCCACTGGCCAAACG
  TGGCACTTTGAAGCCACGACGTATGAGGAGCGGGATGCCTGGGTCCAAGCCATCCAGAGCCAGATCCTGG
  CCAGCCTGCAGTCATGCGAGAGCAGTAAAAGCAAGTCCCAGCTGACCAGCCAGAGCGAGGCCATGGCCCT
  GCAGTCGATCCAAAACATGCGTGGGAACGCCCACTGTGTGGACTGTGAGACCCAGAATCCTAAGTGGGCC
  AGTTTGAACTTGGGAGTCCTCATGTGTATTGAATGCTCAGGTATCCACCGCAGTCTTGGCCCCCACCTTT
  CCCGTGTGCGATCTCTGGAGCTGGATGACTGGCCAGTTGAGCTCAGGAAGGTTATGTCATCTATTGTCAA
  TGACCTAGCCAACAGCATCTGGGAAGGGAGCAGCCAGGGGCAGACAAAACCCTCAGAAAAGTCCACGAGG
  GAAGAGAAGGAACGGTGGATCCGTTCCAAATATGAGGAGAAGCTCTTTCTGGCCCCACTACCCTGCACTG
  AGCTGTCCCTGGGCCAGCAGCTGCTGCGGGCCACCGCTGATGAGGACCTGCAGACAGCCATCCTGCTGCT
  GGCACATGGCTCCTGTGAGGAGGTGAACGAGACCTGTGGGGAGGGAGACGGCTGCACGGCGCTCCATCTG
  GCCTGCCGCAAGGGGAATGTGGTCCTGGCGCAGCTCCTGATCTGGTACGGGGTGGACGTCATGGCCCGAG
  ATGCCCACGGGAACACAGCGCTGACCTACGCCCGGCAGGCCTCCAGCCAGGAGTGCATCAACGTGCTTCT
  GCAGTACGGCTGCCCCGACGAGTGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222390 representing NM_001077665
  Red=Cloning site Green=Tags(s)
  
  MGNILTCRVHPSVSLEFDQQQGSVCPSESETYEAGARDRMAGAPMAAAVQPAEVTVEVGEDLHMHHVRDR
  EMPEALEFNLSANPESSTIFQRNSQTEALEFNPSANPEASTIFQRNSQTDVVEIRRSNCTNHVSAVRFSQ
  QYSLCSTIFLDDSTAIQHYLTMTIISVTLEIPHHITQRDADRTLSIPDEQLHSFAVSTVHIMKKRNGGGS
  LNNYSSSIPSTPSTSQEDPQFSVPPTANTPTPVCKRSMRWSNLFTSEKGSDPDKERKAPENHADTIGSGR
  AIPIKQGMLLKRSGKWLKTWKKKYVTLCSNGMLTYYSSLGDYMKNIHKKEIDLQTSTIKVPGKWPSLATS
  ACTPISSSKSNGLSKDMDTGLGDSICFSPSISSTTSPKLNPPPSPHANKKKHLKKKSTNNFMIVSATGQT
  WHFEATTYEERDAWVQAIQSQILASLQSCESSKSKSQLTSQSEAMALQSIQNMRGNAHCVDCETQNPKWA
  SLNLGVLMCIECSGIHRSLGPHLSRVRSLELDDWPVELRKVMSSIVNDLANSIWEGSSQGQTKPSEKSTR
  EEKERWIRSKYEEKLFLAPLPCTELSLGQQLLRATADEDLQTAILLLAHGSCEEVNETCGEGDGCTALHL
  ACRKGNVVLAQLLIWYGVDVMARDAHGNTALTYARQASSQECINVLLQYGCPDECV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077665
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077665.2, NP_001071133.2
• RefSeq Size: 2772 bp
• RefSeq ORF: 2061 bp
• Locus ID: 414189
• UniProt ID: Q5VW22
• Cytogenetics: 10q11.23
• MW: 75.5 kDa
• Gene Summary: Putative GTPase-activating protein.[UniProtKB/Swiss-Prot Function]