CBY2 (NM_152719) Human Tagged ORF Clone

CAT#: RC222325

SPERT (Myc-DDK-tagged)-Human spermatid associated (SPERT)

Product Images

Circular map for RC222325

• 

  Western blot validation of overexpression lysate (Cat# LY407339) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222325 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBY2
• Synonyms: NURIT; SPERT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222325 representing NM_152719
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACCAGAGGGGTTGAAATGTAGGATGGAGGAATCCAATGCAGAGAGGGGCACAGCCGAACCCTTCC
  CGAGGCTCCACAACTTGTACAGCACCCCTCGCTGCGCGCAGCAGGCCGCCCTGCCCCGGCTGAGCCGCAG
  GATGGCGAGCCAGCACTCCTATCCACTGAACCGCTTCTCCTCCGTGCCTTTAGACCCCATGGAGCGCCCC
  ATGTCCCAGGCCGACCTGGAGCTGGACTACAACCCGCCGCGGGTGCAGCTCAGCGACGAGATGTTCGTGT
  TCCAGGACGGGCGCTGGGTAAATGAGAACTGCCGCCTGCAGTCTCCCTACTTCTCCCCATCCGCCTCCTT
  CCACCACAAGCTGCACCACAAGAGGCTGGCCAAGGAGTGCATGCTGCAGGAGGAGAACAAGTCTCTGCGG
  GAGGAGAACAAGGCCCTGCGCGAGGAGAACCGGATGCTCAGCAAGGAGAACAAGATCCTACAGGTCTTCT
  GGGAGGAGCACAAGGCCTCGCTGGGCCGAGAGGAGAGCCGGGCCCCCTCGCCACTGCTGCACAAAGACAG
  CGCGTCCCTGGAGGTGGTGAAGAAGGACCACGTCGCCCTGCAGGTGCCCCGTGGCAAGGAGGACAGCACC
  CTGCAGCTCCTCCGGGAGGAGAATCGCGCGCTGCAGCAGCTGCTGGAGCAGAAACAGGCCTACTGGGCGC
  AGGCAGAGGACACGGCCGCCCCTGCCGAGGAAAGCAAGCCCGCCCCCTCACCCCACGAGGAGCCCTGCAG
  CCCCGGGCTGCTGCAGGACCAGGGCTCCGGCCTCTCCTCCCGCTTCGAGGAGCCCAAAGGGCCTCCGGCC
  CGGCAGGAGGACTCCAAGGAGCTGCGCGCCCTGCGGAAGATGGTCAGCAACATGTCCGGGCCCTCCGGGG
  AGGAGGAGGCCAAGGTGGGCCCGGGCCTGCCCGACGGCTGCCAGCCCCTGCAGCTGCTGAGAGAGATGAG
  GCAGGCGCTGCAGGCCCTGCTCAAGGAGAACCGGCTCCTGCAGGAGGAGAACAGGACCCTGCAGGTGCTA
  CGGGCAGAGCACAGGGGCTTCCAGGAGGAGAACAAGGCCCTGTGGGAGAACAACAAGCTGAAGCTGCAGC
  AGAAGCTGGTCATTGACACCGTGACCGAGGTCACCGCGCGCATGGAAATGCTCATCGAGGAGCTCTACGC
  CTTCATGCCGGCCAGGAGCCAGGACCCCAAGAAGCCTAGCAGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222325 representing NM_152719
  Red=Cloning site Green=Tags(s)
  
  MQPEGLKCRMEESNAERGTAEPFPRLHNLYSTPRCAQQAALPRLSRRMASQHSYPLNRFSSVPLDPMERP
  MSQADLELDYNPPRVQLSDEMFVFQDGRWVNENCRLQSPYFSPSASFHHKLHHKRLAKECMLQEENKSLR
  EENKALREENRMLSKENKILQVFWEEHKASLGREESRAPSPLLHKDSASLEVVKKDHVALQVPRGKEDST
  LQLLREENRALQQLLEQKQAYWAQAEDTAAPAEESKPAPSPHEEPCSPGLLQDQGSGLSSRFEEPKGPPA
  RQEDSKELRALRKMVSNMSGPSGEEEAKVGPGLPDGCQPLQLLREMRQALQALLKENRLLQEENRTLQVL
  RAEHRGFQEENKALWENNKLKLQQKLVIDTVTEVTARMEMLIEELYAFMPARSQDPKKPSRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152719
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1613 bp
• RefSeq ORF: 1347 bp
• Locus ID: 220082
• UniProt ID: Q8NA61
• Cytogenetics: 13q14.13
• MW: 51.4 kDa