BTBD16 (NM_144587) Human Tagged ORF Clone

CAT#: RC222309

BTBD16 (Myc-DDK-tagged)-Human BTB (POZ) domain containing 16 (BTBD16)

Product Images

Circular map for RC222309

• 

  Western blot validation of overexpression lysate (Cat# LY408234) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222309 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BTBD16
• Synonyms: C10orf87
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222309 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAATGTCGAACACGCAGCACAAAGCTCGGCTGGAACGCCGGGTCACTGGCTCAACCAACCGGTGGC
  GTTTGCCCAAACAGCCTTTCTCTGGGGACCTGCTCTCACTTTCCCAGATGTGCAAGGCTCTGAGCATAGA
  CTTTGAGGAAGCTTTGAGGAACCCAGACAGGTTATGCATTTCACAAATCCAGAAGTTTTTCTTTGAGAAT
  TTCAAGAACAAGGACATCCAAAGTGGGGAAGCAGATGTGATTCTCGAGTGCCTGGGCTTCAAATGGGAGC
  TCCATCAGCCCCAGCTTTTTCAGTCTGAGACCTTGGCCAAGCTCTACCTGAAAGCCCTGGCGCAGGGCAC
  CACACACCCCCTGAGGGAGCTGGAGGAGCTTCTGCGAGCTCAATCACCTAAGAAGACCAAAGAAAAATCC
  CCTGCAAAGAGGATCATCATTTCCTTGAAGATCAATGACCCACTGGTCACTAAAGTCGCCTTCGCCACGG
  CCCTGAAGAACCTCTACATGAGTGAGGTGGAGATTAACTTGGAAGACCTACTGGGAGTGCTGGCTTCCGC
  CCACATCCTCCAGTTCAGTGGCCTGTTTCAAAGGTGCGTGGATGTGATGATAGCCAGACTCAAGCCAAGC
  ACCATCAAGAAATTCTACGAGGCCGGCTGCAAGTACAAGGAAGAGCAGCTCACCACCGGCTGCGAGAAGT
  GGCTGGAAATGAACTTGGTTCCTCTAGGGGGGACGCAGATCCACCTCCACAAAATCCCACAGGACCTGCT
  CCACAAAGTGCTGAAGTCCCCCAGGTTATTTACCTTTAGTGAATTCCATCTTCTGAAAACAATGCTTTTG
  TGGGTCTTCTTGCAACTGAACTACAAGATTCAGGCAATTCCGACTTATGAAACCGTGATGACATTTTTTA
  AGAGCTTTCCTGAGAACTGTTGCTTTCTGGACCGGGACATAGGACGGAGCTTGAGGCCGCTCTTCCTCTG
  CTTGCGTCTGCACGGCATCACCAAAGGCAAGGATCTGGAGGTGCTGCGGCACCTTAACTTCTTCCCAGAG
  TCATGGCTCGACCAGGTTACAGTCAACCATTACCACGCACTGGAGAATGGGGGCGACATGGTCCACCTGA
  AAGATCTTAACACCCAGGCTGTGAGATTTGGGCTGCTCTTTAACCAGGAGAATACAACTTATTCGAAAAC
  GATTGCTCTATATGGATTCTTCTTTAAGATAAAGGGACTCAAACATGATACTACCTCTTATAGTTTTTAC
  ATGCAGAGAATAAAGCACACAGACCTGGAATCTCCCTCTGCGGTCTACGAGCACAACCACGTCAGCCTGC
  GAGCGGCACGCCTGGTGAAGTATGAGATCAGAGCAGAGGCCCTGGTTGACGGCAAGTGGCAGGAGTTCAG
  GACAAACCAGATCAAGCAGAAGTTTGGGTTGACCACGTCATCCTGCAAAAGCCATACCTTGAAAATCCAA
  ACTGTGGGCATCCCAATCTATGTAAGTTTTGCATTCATCTTCCCAGCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222309 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIMSNTQHKARLERRVTGSTNRWRLPKQPFSGDLLSLSQMCKALSIDFEEALRNPDRLCISQIQKFFFEN
  FKNKDIQSGEADVILECLGFKWELHQPQLFQSETLAKLYLKALAQGTTHPLRELEELLRAQSPKKTKEKS
  PAKRIIISLKINDPLVTKVAFATALKNLYMSEVEINLEDLLGVLASAHILQFSGLFQRCVDVMIARLKPS
  TIKKFYEAGCKYKEEQLTTGCEKWLEMNLVPLGGTQIHLHKIPQDLLHKVLKSPRLFTFSEFHLLKTMLL
  WVFLQLNYKIQAIPTYETVMTFFKSFPENCCFLDRDIGRSLRPLFLCLRLHGITKGKDLEVLRHLNFFPE
  SWLDQVTVNHYHALENGGDMVHLKDLNTQAVRFGLLFNQENTTYSKTIALYGFFFKIKGLKHDTTSYSFY
  MQRIKHTDLESPSAVYEHNHVSLRAARLVKYEIRAEALVDGKWQEFRTNQIKQKFGLTTSSCKSHTLKIQ
  TVGIPIYVSFAFIFPAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144587
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144587.5
• RefSeq Size: 1848 bp
• RefSeq ORF: 1521 bp
• Locus ID: 118663
• UniProt ID: Q32M84
• Cytogenetics: 10q26.13
• MW: 58.6 kDa
• Gene Summary: This gene encodes a protein that contains a BTB/POZ domain. This domain mediates protein-protein interactions. A mutation in this gene may be associated with bipolar disorder. [provided by RefSeq, Sep 2016]