POLR1F (NM_001002926) Human Tagged ORF Clone

CAT#: RC222203

TWISTNB (Myc-DDK-tagged)-Human TWIST neighbor (TWISTNB)

Product Images

Circular map for RC222203

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  Western blot validation of overexpression lysate (Cat# LY424120) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222203 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TWISTNB protein (Cat# TP322203). The protein was produced from HEK293T cells transfected with TWISTNB cDNA clone (Cat# RC222203) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLR1F
• Synonyms: A43; RPA43; TWISTNB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222203 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGGTTGCTCAGAGGCGCCGCGGCCAGCGGCGGCTTCTGATGGGTCTCTGGTAGGGCAGGCTG
  GCGTCCTGCCTTGCCTAGAGTTGCCGACTTATGCCGCTGCTTGTGCGCTGGTGAACAGTCGCTACTCATG
  CCTGGTGGCCGGGCCGCACCAAAGGCACATCGCGCTGTCGCCCCGCTACCTTAACAGGAAACGCACCGGC
  ATTCGAGAACAGCTTGATGCGGAGCTCCTTCGCTATTCTGAGAGCCTTTTAGGTGTCCCTATTGCATATG
  ATAACATCAAAGTTGTGGGAGAGCTTGGAGATATTTATGATGATCAAGGACACATTCATCTTAACATTGA
  AGCCGATTTTGTTATTTTCTGCCCTGAACCAGGGCAGAAGCTTATGGGTATAGTTAATAAAGTGTCTTCT
  AGCCACATTGGCTGTTTAGTACATGGGTGTTTCAATGCCTCCATTCCTAAACCTGAGCAGTTGTCAGCTG
  AGCAGTGGCAAACCATGGAGATAAACATGGGTGATGAACTAGAATTTGAAGTATTTCGTTTAGACTCAGA
  TGCTGCTGGAGTATTCTGCATTCGGGGAAAACTAAATATCACAAGTTTACAATTCAAGCGCTCTGAAGTT
  TCTGAAGAAGTTACAGAAAATGGCACTGAGGAAGCTGCTAAAAAACCTAAAAAGAAGAAAAAGAAGAAAG
  ACCCAGAGACATATGAAGTGGACAGTGGTACCACAAAGCTAGCAGATGATGCAGATGACACTCCAATGGA
  AGAGTCAGCCCTGCAGAATACTAATAATGCGAATGGCATCTGGGAGGAGGAGCCAAAGAAAAAGAAGAAG
  AAGAAAAAGCACCAGGAAGTTCAGGACCAGGACCCTGTTTTCCAAGGCAGTGACTCCAGTGGTTACCAAA
  GTGACCATAAAAAGAAAAAAAAGAAAAGAAAACACAGTGAAGAGGCCGAATTTACCCCACCTTTGAAATG
  CTCACCAAAAAGAAAAGGGAAAAGTAATTTTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222203 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGCSEAPRPAAASDGSLVGQAGVLPCLELPTYAAACALVNSRYSCLVAGPHQRHIALSPRYLNRKRTG
  IREQLDAELLRYSESLLGVPIAYDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQKLMGIVNKVSS
  SHIGCLVHGCFNASIPKPEQLSAEQWQTMEINMGDELEFEVFRLDSDAAGVFCIRGKLNITSLQFKRSEV
  SEEVTENGTEEAAKKPKKKKKKKDPETYEVDSGTTKLADDADDTPMEESALQNTNNANGIWEEEPKKKKK
  KKKHQEVQDQDPVFQGSDSSGYQSDHKKKKKKRKHSEEAEFTPPLKCSPKRKGKSNFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002926
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002926.2
• RefSeq Size: 3892 bp
• RefSeq ORF: 1017 bp
• Locus ID: 221830
• UniProt ID: Q3B726
• Cytogenetics: 7p21.1
• MW: 37.4 kDa
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.[UniProtKB/Swiss-Prot Function]