XYLT1 (NM_022166) Human Tagged ORF Clone

CAT#: RC222133

XYLT1 (Myc-DDK-tagged)-Human xylosyltransferase I (XYLT1)

Product Images

Circular map for RC222133

• 

  Western blot validation of overexpression lysate (Cat# LY411721) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222133 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XYLT1
• Synonyms: DBQD2; PXYLT1; XT-I; XT1; XTI; xylT-I; XYLTI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222133 representing NM_022166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCGGCGCCGTGCGCCCGGAGGCTGGCCCGGCGCTCGCACTCGGCGCTGCTCGCGGCGCTCACGG
  TGCTGCTGCTGCAGACGCTGGTCGTGTGGAATTTCAGCAGCCTCGACTCCGGGGCCGGGGAGCGCCGCGG
  GGGCGCAGCGGTCGGCGGCGGGGAGCAGCCGCCCCCGGCCCCGGCCCCGCGCCGGGAGCGCCGGGACCTG
  CCCGCCGAGCCGGCTGCAGCCCGAGGAGGAGGAGGAGGCGGCGGCGGAGGAGGAGGAGGACGGGGGCCCC
  AGGCGCGGGCGCGGGGAGGCGGCCCCGGAGAACCGCGGGGACAGCAGCCGGCCAGCCGGGGGGCACTGCC
  CGCCCGGGCTCTGGATCCACACCCAAGTCCGCTCATCACCCTGGAGACTCAGGATGGCTACTTTTCTCAT
  CGGCCGAAAGAGAAAGTGCGAACAGACAGCAACAACGAGAACTCTGTCCCCAAAGACTTTGAGAATGTGG
  ACAACAGCAACTTCGCACCCAGGACTCAAAAGCAGAAGCACCAGCCTGAGTTGGCGAAGAAGCCACCGAG
  TAGACAGAAGGAGCTTTTGAAAAGGAAGCTGGAACAGCAGGAGAAAGGAAAAGGACATACATTCCCTGGG
  AAAGGCCCCGGTGAGGTGCTGCCTCCCGGGGACAGAGCCGCAGCCAACAGCAGCCACGGGAAGGATGTGT
  CCAGACCGCCTCATGCCAGGAAAACTGGGGGCAGCTCCCCCGAGACCAAGTATGACCAGCCCCCTAAGTG
  TGACATCTCAGGCAAGGAGGCCATCTCTGCCCTGTCCCGTGCTAAGTCCAAGCACTGCCGCCAGGAGATT
  GGGGAGACTTACTGCCGCCACAAGTTAGGGCTGCTGATGCCTGAGAAGGTGACTCGGTTCTGCCCCCTCG
  AGGGTAAAGCCAACAAGAACGTGCAGTGGGACGAGGACTCCGTGGAGTACATGCCAGCCAACCCGGTCAG
  AATCGCCTTTGTCCTGGTGGTCCACGGCCGTGCCTCTCGGCAGTTGCAGCGCATGTTCAAGGCCATCTAC
  CACAAAGACCACTTCTACTACATCCACGTGGACAAGCGCTCTAATTACCTGCATCGGCAAGTGCTCCAGG
  TCTCCAGGCAGTACAGCAATGTCCGCGTCACCCCCTGGAGAATGGCCACCATCTGGGGAGGAGCCAGCCT
  CCTGTCCACCTACCTGCAGAGCATGCGGGACCTCCTGGAGATGACCGACTGGCCCTGGGACTTCTTCATC
  AACCTGAGTGCGGCCGACTACCCCATCAGGACAAATGACCAGTTGGTGGCGTTTCTCTCCCGATACCGAG
  ATATGAATTTCTTGAAGTCACACGGCCGGGACAATGCAAGGTTCATTCGGAAGCAGGGCCTGGATCGGCT
  CTTCCTGGAGTGCGACGCTCACATGTGGCGCCTGGGAGATCGGCGGATCCCAGAGGGCATTGCCGTGGAT
  GGCGGTTCGGACTGGTTCCTGCTGAACCGGAGGTTTGTAGAATATGTGACCTTCTCCACAGACGATCTGG
  TGACCAAGATGAAACAGTTCTACTCCTACACCCTGCTTCCTGCTGAGTCCTTCTTCCATACGGTCCTGGA
  GAACAGCCCCCACTGCGACACCATGGTGGACAACAACCTGCGCATCACCAACTGGAATCGCAAGCTGGGC
  TGCAAGTGCCAGTACAAGCACATCGTGGACTGGTGCGGCTGCTCCCCCAATGACTTCAAGCCGCAGGACT
  TCCACCGCTTCCAGCAGACAGCCCGGCCTACCTTCTTTGCCCGCAAGTTTGAAGCCGTGGTGAATCAGGA
  AATCATTGGGCAGCTGGACTATTACCTGTACGGGAACTACCCTGCAGGTACCCCGGGCCTGCGCTCCTAC
  TGGGAGAATGTCTACGATGAGCCTGACGGCATCCACAGCCTGAGCGACGTGACACTCACCTTGTACCACT
  CCTTTGCCCGCCTGGGTCTTCGACGGGCCGAGACGTCCCTGCACACGGATGGGGAGAACAGCTGCCGATA
  CTACCCAATGGGCCACCCAGCATCTGTGCACCTCTACTTCCTTGCTGACCGCTTCCAGGGCTTTCTGATC
  AAGCATCATGCTACCAATCTGGCTGTGAGCAAACTAGAGACTCTGGAGACCTGGGTGATGCCGAAAAAAG
  TCTTCAAGATCGCAAGCCCACCCAGTGACTTTGGGAGGCTTCAGTTTTCCGAGGTCGGCACTGACTGGGA
  TGCCAAGGAGAGGCTATTCCGCAACTTTGGGGGTCTTCTGGGGCCCATGGATGAGCCGGTGGGTATGCAG
  AAGTGGGGGAAGGGACCTAATGTGACCGTGACCGTCATTTGGGTGGATCCCGTCAATGTCATCGCAGCCA
  CCTACGACATCCTCATTGAGTCCACTGCCGAATTCACACACTACAAGCCCCCTTTGAACTTGCCCCTGAG
  GCCTGGGGTCTGGACAGTGAAAATTCTCCACCACTGGGTGCCAGTTGCAGAGACCAAATTCCTCGTTGCG
  CCTCTGACCTTCTCGAACAGGCAGCCCATCAAACCTGAGGAGGCACTGAAGCTGCACAATGGGCCCCTCC
  GCAATGCCTACATGGAGCAGAGCTTCCAGAGCCTAAACCCCGTCCTCAGCCTGCCCATCAACCCCGCCCA
  GGTGGAACAGGCACGGAGGAACGCAGCCTCCACGGGCACAGCGCTGGAGGGATGGCTGGACTCGTTGGTG
  GGCGGGATGTGGACTGCCATGGACATCTGTGCCACGGGCCCCACAGCCTGCCCGGTCATGCAGACCTGCA
  GCCAGACGGCCTGGAGCTCCTTCAGCCCTGACCCCAAGTCGGAGCTGGGGGCAGTCAAACCTGATGGCCG
  GCTCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222133 representing NM_022166
  Red=Cloning site Green=Tags(s)
  
  MVAAPCARRLARRSHSALLAALTVLLLQTLVVWNFSSLDSGAGERRGGAAVGGGEQPPPAPAPRRERRDL
  PAEPAAARGGGGGGGGGGGGRGPQARARGGGPGEPRGQQPASRGALPARALDPHPSPLITLETQDGYFSH
  RPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPPSRQKELLKRKLEQQEKGKGHTFPG
  KGPGEVLPPGDRAAANSSHGKDVSRPPHARKTGGSSPETKYDQPPKCDISGKEAISALSRAKSKHCRQEI
  GETYCRHKLGLLMPEKVTRFCPLEGKANKNVQWDEDSVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIY
  HKDHFYYIHVDKRSNYLHRQVLQVSRQYSNVRVTPWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFI
  NLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDAHMWRLGDRRIPEGIAVD
  GGSDWFLLNRRFVEYVTFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSPHCDTMVDNNLRITNWNRKLG
  CKCQYKHIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKFEAVVNQEIIGQLDYYLYGNYPAGTPGLRSY
  WENVYDEPDGIHSLSDVTLTLYHSFARLGLRRAETSLHTDGENSCRYYPMGHPASVHLYFLADRFQGFLI
  KHHATNLAVSKLETLETWVMPKKVFKIASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQ
  KWGKGPNVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVA
  PLTFSNRQPIKPEEALKLHNGPLRNAYMEQSFQSLNPVLSLPINPAQVEQARRNAASTGTALEGWLDSLV
  GGMWTAMDICATGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022166
• ORF Size: 2877 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022166.2, NP_071449.1
• RefSeq Size: 4178 bp
• RefSeq ORF: 2880 bp
• Locus ID: 64131
• UniProt ID: Q86Y38
• Cytogenetics: 16p12.3
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• MW: 107.4 kDa
• Gene Summary: This locus encodes a xylosyltransferase enzyme. The encoded protein catalyzes transfer of UDP-xylose to serine residues of an acceptor protein substrate. This transfer reaction is necessary for biosynthesis of glycosaminoglycan chains. Mutations in this gene have been associated with increased severity of pseudoxanthoma elasticum.[provided by RefSeq, Nov 2009]