ACTL10 (NM_001024675) Human Tagged ORF Clone

CAT#: RC221959

ACTL10 (Myc-DDK-tagged)-Human chromosome 20 open reading frame 134 (C20orf134)

Product Images

Circular map for RC221959

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  Western blot validation of overexpression lysate (Cat# LY422532) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221959 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACTL10
• Synonyms: C20orf134; dJ63M2.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221959 representing NM_001024675
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGCACCGCGTTGCTGGCGCTCTGCTCCACCGGCGCGTTCAGCGGGCTGGCCGTGGAGGCGGGCG
  CGGGCGTGTGCCACGCCACGCCCATCTACGCGGGTCACTCGTGGCACCAGGCCACCTTCCGACTGAACGT
  GGCAGGCAGCACCCTGTCGCGCTACCTGCGGGATCTGCTGGTGGCGGCGAACCCTGACCTCTTGCAGCAG
  GCCCTGCCCCGCAAGGCCATCACACATCTCAAGAAGCGCAGCTGCTACGTGTCCCTGGACTTCGAGGGCG
  ACCTCCGCGACCCCGCCCGCCACCATCCGGCCAGTTTCAGCGTGGGTAACGGGTGCTGCGTCTGCCTCAG
  CAGTGAGCGCTTCCGCTGCCCCGAACCCATCTTCCAGCCGGGCCTGCTGGGCCAGGCTGAGCAGGGGCTG
  CCCGCGCTGGCCTTCCGGGCGCTGCAGAAGATGCCCAAAACGCTGCGGACACGCCTGGCAGACACCGTGG
  TGCTAGCCGGCGGCTCCACACTGTTTCCTGGCTTCGCCGAGCGCCTGGACAAGGAGCTGGAGGCGCAGTG
  CCGGCGGCACGGCTACGCGGCCCTGCGGCCCCACCTGGTGGCCAAGCATGGGCGTGGCATGGCTGTGTGG
  ACCGGCGGCTCCATGGTGGCCTCCCTGCACTCCTTCCAGCGCCGCTGGATAACTCGGGCCATGTACCAGG
  AGTGTGGCTCCAGGCTGCTGTACGATGTGTTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221959 representing NM_001024675
  Red=Cloning site Green=Tags(s)
  
  MASTALLALCSTGAFSGLAVEAGAGVCHATPIYAGHSWHQATFRLNVAGSTLSRYLRDLLVAANPDLLQQ
  ALPRKAITHLKKRSCYVSLDFEGDLRDPARHHPASFSVGNGCCVCLSSERFRCPEPIFQPGLLGQAEQGL
  PALAFRALQKMPKTLRTRLADTVVLAGGSTLFPGFAERLDKELEAQCRRHGYAALRPHLVAKHGRGMAVW
  TGGSMVASLHSFQRRWITRAMYQECGSRLLYDVFN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024675
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024675.2
• RefSeq Size: 2028 bp
• RefSeq ORF: 738 bp
• Locus ID: 170487
• UniProt ID: Q5JWF8
• Cytogenetics: 20q11.22
• Protein Families: Secreted Protein
• MW: 26.8 kDa