HOXB8 (NM_024016) Human Tagged ORF Clone

CAT#: RC221896

HOXB8 (Myc-DDK-tagged)-Human homeobox B8 (HOXB8)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_024016" in other vectors (6)

Reconstitution Protocol

USD 450.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HOXB8
Synonyms Hox-2.4; HOX2; HOX2D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221896 representing NM_024016
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCTTATTTCGTCAACTCACTGTTCTCCAAATACAAAACCGGGGAGTCCCTGCGCCCCAATTATT
ATGACTGCGGCTTCGCCCAGGACCTGGGCGGCCGACCCACCGTGGTGTACGGTCCCAGCAGCGGCGGCAG
CTTCCAGCACCCGTCGCAAATCCAGGAGTTCTACCACGGGCCGTCGTCGCTGTCCACGGCTCCCTACCAG
CAGAACCCGTGCGCCGTGGCGTGCCACGGGGACCCCGGCAATTTCTACGGCTACGACCCGCTGCAACGCC
AGAGCCTATTCGGTGCGCAGGATCCAGACCTGGTGCAGTACGCAGACTGCAAGCTTGCCGCCGCCAGCGG
CCTGGGCGAGGAGGCCGAGGGCTCCGAGCAGAGCCCGTCGCCCACACAGCTCTTCCCCTGGATGCGCCCG
CAAGCCGCCGGACGCAGGCGAGGCCGACAGACCTACAGCCGCTACCAGACCCTGGAGCTGGAGAAGGAGT
TCCTATTTAATCCCTATCTGACTCGTAAGCGGCGAATCGAGGTATCGCACGCCCTGGGACTGACAGAGAG
ACAGGTCAAAATCTGGTTCCAGAACCGGAGGATGAAGTGGAAAAAAGAGAACAACAAAGACAAGTTCCCC
AGCAGCAAATGCGAGCAGGAGGAGCTGGAGAAACAGAAGCTGGAGCGGGCCCCAGAGGCGGCGGACGAGG
GCGACGCGCAGAAGGGCGACAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC221896 representing NM_024016
Red=Cloning site Green=Tags(s)

MSSYFVNSLFSKYKTGESLRPNYYDCGFAQDLGGRPTVVYGPSSGGSFQHPSQIQEFYHGPSSLSTAPYQ
QNPCAVACHGDPGNFYGYDPLQRQSLFGAQDPDLVQYADCKLAAASGLGEEAEGSEQSPSPTQLFPWMRP
QAAGRRRGRQTYSRYQTLELEKEFLFNPYLTRKRRIEVSHALGLTERQVKIWFQNRRMKWKKENNKDKFP
SSKCEQEELEKQKLERAPEAADEGDAQKGDKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024016
ORF Size 726 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024016.4
RefSeq Size 1823 bp
RefSeq ORF 732 bp
Locus ID 3218
UniProt ID P17481
Cytogenetics 17q21.32
Protein Families ES Cell Differentiation/IPS
MW 27.4 kDa
Gene Summary This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Increased expression of this gene is associated with colorectal cancer. Mice that have had the murine ortholog of this gene knocked out exhibit an excessive pathologic grooming behavior. This behavior is similar to the behavior of humans suffering from the obsessive-compulsive spectrum disorder trichotillomania. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.