PCB (PC) (NM_000920) Human Tagged ORF Clone

CAT#: RC221895

PC (Myc-DDK-tagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC221895

• 

  Western blot validation of overexpression lysate (Cat# LY420837) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221952 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PC protein (Cat# TP321895). The protein was produced from HEK293T cells transfected with PC cDNA clone (Cat# RC221895) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCB
• Synonyms: PCB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221895 representing NM_000920
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGGGAATCCGCCGAACCTCCACCGCCCCCG
  CTGCCTCCCCAAATGTCCGGCGCCTGGAGTATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGA
  GATTGCCATCCGTGTGTTCCGGGCCTGCACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAG
  GACACGGGCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCGTGC
  AGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGCACCCTGG
  CTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAGGGGTCCGGTTTATTGGG
  CCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGGTGGAGGCCCGGGCCATCGCCATTGCTGCGGGTG
  TTCCCGTTGTCCCTGGCACAGATGCCCCCATCACGTCCCTGCATGAGGCCCACGAGTTCTCCAACACCTA
  CGGCTTCCCCATCATCTTCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTAC
  GAGGAGCTGGAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGT
  TTGTGGAGAAGTTCATCGAGAAGCCACGGCACATCGAGGTGCAGATCTTGGGGGACCAGTATGGGAACAT
  CCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGGCGGCACCAGAAGGTGGTCGAGATTGCCCCCGCC
  GCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCAGCGACTCTGTGAAACTCGCTAAACAGGTGGGCT
  ACGAGAACGCAGGCACCGTGGAGTTCCTGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAACTC
  CCGCCTGCAGGTGGAGCACACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCAC
  GTGGCTGAGGGCAGGAGCCTACCCGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAACGGGTGTGCCA
  TCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGCTTCCAGCCGGACACCGGCCGCATTGAGGTGTT
  CCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCTTCCAAGGAGCCGTCATCTCGCCC
  CACTACGACTCCCTGCTGGTCAAAGTCATTGCCCACGGCAAAGACCACCCCACGGCCGCCACCAAGATGA
  GCAGGGCCCTTGCGGAGTTCCGCGTCCGAGGTGTGAAGACCAACATCGCCTTCCTGCAGAATGTGCTCAA
  CAACCAGCAGTTCCTGGCAGGCACTGTGGACACCCAGTTCATCGACGAGAACCCAGAGCTGTTCCAGCTG
  CGGCCTGCACAGAACCGGGCCCAAAAGCTGTTGCACTACCTCGGCCATGTCATGGTAAACGGTCCAACCA
  CCCCGATTCCCGTCAAGGCCAGCCCCAGCCCCACGGACCCCGTTGTCCCTGCAGTGCCCATAGGCCCGCC
  CCCGGCTGGTTTCAGAGACATCCTGCTGCGAGAGGGGCCTGAGGGCTTTGCTCGAGCTGTGCGGAACCAC
  CCGGGGCTGCTGCTGATGGACACGACCTTCAGGGACGCCCACCAGTCACTGCTGGCCACTCGTGTGCGCA
  CCCACGATCTCAAAAAGATCGCCCCCTATGTTGCCCACAACTTCAGCAAGCTCTTCAGCATGGAGAACTG
  GGGAGGAGCCACGTTTGACGTCGCCATGCGCTTCCTGTATGAGTGCCCCTGGCGGCGGCTGCAGGAGCTC
  CGGGAGCTCATCCCCAACATCCCTTTCCAGATGCTGCTGCGGGGGGCCAATGCTGTGGGCTACACCAACT
  ACCCAGACAACGTGGTCTTCAAGTTCTGTGAAGTGGCCAAAGAGAATGGCATGGATGTCTTCCGTGTGTT
  TGACTCCCTCAACTACTTGCCCAACATGCTGCTGGGCATGGAGGCGGCAGGAAGTGCCGGAGGCGTGGTG
  GAGGCTGCCATCTCATACACGGGCGACGTGGCCGACCCCAGCCGCACCAAGTACTCACTGCAGTACTACA
  TGGGCTTGGCCGAAGAGCTGGTGCGAGCTGGCACCCACATCCTGTGCATCAAGGACATGGCCGGGCTGCT
  GAAGCCCACGGCCTGCACCATGCTGGTCAGCTCCCTCCGGGACCGCTTCCCCGACCTCCCACTGCACATC
  CACACCCACGACACGTCAGGGGCAGGCGTGGCAGCCATGCTGGCCTGTGCCCAGGCTGGAGCTGATGTGG
  TGGATGTGGCAGCTGATTCCATGTCTGGGATGACTTCACAGCCCAGCATGGGGGCCCTGGTGGCCTGTAC
  CAGAGGGACTCCCCTGGACACAGAGGTGCCCATGGAGCGCGTGTTTGACTACAGTGAGTACTGGGAGGGG
  GCTCGGGGACTGTACGCGGCCTTCGACTGCACGGCCACCATGAAGTCTGGCAACTCGGACGTGTATGAAA
  ATGAGATCCCAGGGGGCCAGTACACCAACCTGCACTTCCAGGCCCACAGCATGGGGCTTGGCTCCAAGTT
  CAAGGAGGTCAAGAAGGCCTATGTGGAGGCCAACCAGATGCTGGGCGATCTCATCAAGGTGACGCCCTCC
  TCCAAGATCGTGGGGGACCTGGCCCAGTTTATGGTGCAGAATGGATTGAGCCGGGCAGAGGCCGAAGCTC
  AGGCGGAAGAGCTGTCCTTTCCCCGCTCCGTGGTGGAGTTCCTGCAGGGCTACATCGGTGTCCCCCATGG
  GGGGTTCCCCGAACCCTTTCGCTCTAAGGTACTGAAGGACCTGCCAAGGGTGGAGGGGCGGCCTGGAGCC
  TCCCTCCCTCCCCTGGATCTGCAGGCACTGGAGAAGGAGCTGGTAGACCGGCATGGGGAGGAGGTGACGC
  CGGAAGATGTGCTCTCAGCAGCTATGTACCCCGATGTGTTTGCCCACTTCAAGGACTTCACTGCCACCTT
  TGGCCCCCTGGATAGCCTGAATACTCGCCTCTTCCTGCAGGGACCCAAGATCGCAGAGGAGTTTGAGGTG
  GAGCTGGAGCGGGGCAAGACGCTGCACATCAAAGCCCTGGCCGTGAGCGACCTGAACCGGGCCGGCCAGA
  GGCAGGTCTTCTTTGAGCTCAATGGGCAGCTGCGGTCCATCTTGGTCAAGGACACCCAGGCCATGAAGGA
  GATGCACTTCCACCCCAAGGCCCTAAAGGACGTGAAGGGCCAGATCGGGGCGCCCATGCCTGGGAAGGTG
  ATAGACATCAAAGTGGTGGCAGGGGCCAAGGTGGCCAAGGGCCAGCCCCTGTGTGTGCTCAGTGCCATGA
  AGATGGAGACTGTGGTGACCTCACCCATGGAGGGTACTGTCCGCAAGGTTCATGTGACCAAGGACATGAC
  ACTGGAAGGTGACGACCTCATCCTGGAGATCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221895 representing NM_000920
  Red=Cloning site Green=Tags(s)
  
  MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQ
  DTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIG
  PSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSY
  EELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPA
  AHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIH
  VAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISP
  HYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQL
  RPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNH
  PGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQEL
  RELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVV
  EAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHI
  HTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEG
  ARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPS
  SKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGA
  SLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEV
  ELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKV
  IDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000920
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000920.4
• RefSeq Size: 4111 bp
• RefSeq ORF: 3537 bp
• Locus ID: 5091
• UniProt ID: P11498
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pyruvate metabolism
• MW: 129.63 kDa
• Gene Summary: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]