TRAPPC6B (NM_001079537) Human Tagged ORF Clone

CAT#: RC221864

TRAPPC6B (Myc-DDK-tagged)-Human trafficking protein particle complex 6B (TRAPPC6B), transcript variant 1

Product Images

Circular map for RC221864

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRAPPC6B
• Synonyms: NEDMEBA; TPC6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221864 representing NM_001079537
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGAGGCGTTGTTTTTGCTTCTCCATAACGAGATGGTGTCTGGAGTGTACAAGTCCGCGGAGC
  AGGGGGAGGTGGAAAACGGACGATGTATTACTAAGCTGGAAAACATGGGGTTTCGAGTGGGACAAGGATT
  GATAGAAAGGTTTACAAAAGATACTGCAAGGTTCAAGGATGAGTTAGATATCATGAAGTTCATTTGTAAA
  GATTTTTGGACTACGGTATTCAAGAAACAAATCGACAATCTAAGGACAAATCATCAGGGCATCTATGTAC
  TTCAGGACAACAAATTTCGCCTGCTTACTCAGATGTCTGCAGGAAAACAGTATTTAGAACATGCATCTAA
  GTATTTAGCATTTACGTGTGGCTTAATCAGAGGTGGCTTATCAAACTTGGGAATAAAAAGTATTGTAACA
  GCTGAAGTGTCTTCAATGCCTGCTTGCAAATTTCAGGTGATGATACAGAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221864 representing NM_001079537
  Red=Cloning site Green=Tags(s)
  
  MADEALFLLLHNEMVSGVYKSAEQGEVENGRCITKLENMGFRVGQGLIERFTKDTARFKDELDIMKFICK
  DFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLTQMSAGKQYLEHASKYLAFTCGLIRGGLSNLGIKSIVT
  AEVSSMPACKFQVMIQKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079537
• ORF Size: 474 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079537.2
• RefSeq Size: 3364 bp
• RefSeq ORF: 477 bp
• Locus ID: 122553
• UniProt ID: Q86SZ2
• Cytogenetics: 14q21.1
• MW: 17.8 kDa
• Gene Summary: TRAPPC6B is a component of TRAPP complexes, which are tethering complexes involved in vesicle transport (Kummel et al., 2005 [PubMed 16025134]).[supplied by OMIM, Mar 2008]