NKIAMRE (CDKL3) (NM_016508) Human Tagged ORF Clone

CAT#: RC221819

CDKL3 (Myc-DDK-tagged)-Human cyclin-dependent kinase-like 3 (CDKL3), transcript variant 2

Product Images

Circular map for RC221819

• 

  Western blot validation of overexpression lysate (Cat# LY413945) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221819 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NKIAMRE
• Synonyms: NKIAMRE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221819 representing NM_016508
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGTATGAAACCCTTGGAAAAGTGGGAGAGGGAAGTTACGGAACAGTCATGAAATGTAAACATA
  AGAATACTGGGCAGATAGTGGCCATTAAGATATTTTATGAGAGACCAGAACAATCTGTCAACAAAATTGC
  GATGAGAGAAATAAAGTTTCTAAAGCAATTTCATCACGAAAACCTGGTCAATCTGATTGAAGTTTTTAGA
  CAGAAAAAGAAAATTCATTTGGTATTTGAATTTATTGACCACACAGTATTAGATGAGTTACAACATTATT
  GTCATGGACTAGAGAGTAAGCGACTTAGAAAATACCTCTTCCAGATCCTTCGAGCAATTGACTATCTTCA
  CAGTAATAATATCATTCATCGAGATATAAAACCTGAGAATATTTTAGTATCCCAGTCAGGAATTACTAAG
  CTCTGTGATTTTGGTTTTGCACGAACACTAGCAGCTCCTGGGGACATTTATACGGACTATGTGGCCACAC
  GCTGGTATAGAGCTCCCGAATTAGTATTAAAAGATACTTCTTATGGAAAACCTGTGGATATCTGGGCTTT
  GGGCTGTATGATCATTGAGATGGCCACTGGAAATCCCTATCTTCCTAGTAGTTCTGATTTGGATTTACTC
  CATAAAATTGTTTTGAAAGTGGGCAATTTGTCACCTCACTTGCAGAATATCTTTTCCAAGAGCCCCATTT
  TTGCTGGGGTAGTTCTTCCTCAAGTTCAACACCCCAAAAATGCAAGAAAAAAATATCCAAAGCTTAATGG
  ATTGTTGGCAGATATAGTTCATGCTTGTTTACAAATTGATCCTGCTGACAGGATATCATCTAGTGATCTT
  TTGCATCATGAGTATTTTACTAGAGATGGATTTATTGAAAAATTCATGCCAGAACTGAAAGCTAAATTAC
  TGCAGGAAGCAAAAGTCAATTCATTAATAAAGCCAAAAGAGAGTTCTAAAGAAAATGAACTCAGGAAAGA
  TGAAAGAAAAACAGTTTATACCAATACACTGCTAAGTAGTTCAGTTTTGGGAGAGGAAATAGAAAAAGAG
  AAAAAGCCCAAGGAGATCAAAGTCAGAGTTATTAAAGTCAAAGGAGGAAGAGGAGATATCTCAGAACCAA
  AAAAGAAAGAGTATGAAGGTGGACTTGGTCAACAGGATGCAAATGAAAATGTTCATCCTATGTCTCCAGA
  TACAAAACTTGTAACCATTGAACCACCAAACCCTATCAATCCCAGCACTAACTGTAATGGCTTGAAAGAA
  AATCCACATTGCGGAGGTTCTGTGACAATGCCACCCATCAATCTAACTAACAGTAATTTGATGGCTGCAA
  ATCTCAGTTCAAATCTCTTTCACCCCAGTGTGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221819 representing NM_016508
  Red=Cloning site Green=Tags(s)
  
  MEMYETLGKVGEGSYGTVMKCKHKNTGQIVAIKIFYERPEQSVNKIAMREIKFLKQFHHENLVNLIEVFR
  QKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQILRAIDYLHSNNIIHRDIKPENILVSQSGITK
  LCDFGFARTLAAPGDIYTDYVATRWYRAPELVLKDTSYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLL
  HKIVLKVGNLSPHLQNIFSKSPIFAGVVLPQVQHPKNARKKYPKLNGLLADIVHACLQIDPADRISSSDL
  LHHEYFTRDGFIEKFMPELKAKLLQEAKVNSLIKPKESSKENELRKDERKTVYTNTLLSSSVLGEEIEKE
  KKPKEIKVRVIKVKGGRGDISEPKKKEYEGGLGQQDANENVHPMSPDTKLVTIEPPNPINPSTNCNGLKE
  NPHCGGSVTMPPINLTNSNLMAANLSSNLFHPSVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016508
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016508.2, NP_057592.1
• RefSeq Size: 1513 bp
• RefSeq ORF: 1368 bp
• Locus ID: 51265
• UniProt ID: Q8IVW4
• Cytogenetics: 5q31.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 51.4 kDa
• Gene Summary: The protein encoded by this gene is a member of cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This gene was identified as a gene absent in leukemic patients with chromosome 5q deletion. This loss may be an important determinant of dysmyelopoiesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]