ZSWIM5 (NM_020883) Human Tagged ORF Clone

CAT#: RC221559

ZSWIM5 (Myc-DDK-tagged)-Human zinc finger, SWIM-type containing 5 (ZSWIM5)

Product Images

Circular map for RC221559

• 

  Western blot validation of overexpression lysate (Cat# LY412245) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221559 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZSWIM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221559 representing NM_020883
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGAGGTGAGCGAGAGGAGCTGCTCTCGCCGTCACCGGTCTCTCCGGCTAAGCGGCAGTGTT
  CGTGGCCCAGCCCGCAGGCTCATCACCCTCGCGGTTCGCCTGGGGCAGCCGGCGGAGGGGCAGGGGGCGT
  CGGCAGCAGCTGCCTGGTCCTCGGGGCCCGCCCCCACCTGCAGCCGGATTCCTTACTGGACTGCGCCGCC
  AAGACGGTGGCGGAAAAGTGGGCATACGAACGGGTGGAGGAGCGCTTCGAGCGGATCCCGGAGCCCGTGC
  AGCGCCGCATCGTCTACTGGTCCTTCCCGCGGAATGAGCGGGAGATCTGCATGTACTCCAGCTTCCAGTA
  CCGGGGCGGCCCCGGCGCCGGCGCTGCTGGCGGCGCCGCGGGGGCTTCGCCGGCCGAGGAGGGGCCGCAG
  CCACCCCCCGGGGCCGCCGCTCCGGCCGGCTCCGCCCCCGGGGGCGTCGCGGCTGGGGCATCCCCCGGGC
  TGGGCGCAGGGGCCGGCGCGGCCGGCTGCGGTGGCGAGGGGCTCCCGTTCCGCCGGGGCATCCGTCTGCT
  GGACAGCGGCTCCGTGGAGAACGTGCTGCAAGTCGGCTTTCATCTGAGTGGCACAGTAACTGAGCTGGCC
  ACTGCCTCTGAACCAGCAGTGACTTATAAAGTTGCAATCAGTTTTGATCGATGCAAAATCACCTCAGTGA
  CATGTGGCTGTGGGAACAAGGACATATTCTACTGTGCCCATGTGGTAGCACTCTCACTCTACAGGATCCG
  TAAACCAGACCAAGTCAAATTGCGTCTTCCTATCTCCGAAACCCTTTTCCAGATGAATAGGGACCAGCTA
  CAAAAGTTTATTCAATATTTAATCACGGCACACCACACTGAAGTTCTTCCTACTGCACAGAAACTGGCAG
  ATGAGATTCTATCCTCCAACTCAGAAATCAACCAAGTGAATGGTGCCCCTGACCCCACAGCTGGGGCCAG
  CATTGACGATGAGAACTGTTGGCATTTGGATGAAGAACAGGTGAAAGAACAAGTGAAGCTTTTTCTCTCC
  CAGGGCGGTTACTGTGGCTCTGGAAAGCAACTCAACTCAATGTTTGCCAAGGTTCGAGAAATGCTGCGGA
  TGAGAGATTCCAATGGAGCAAGGATGCTGACACTAATAACTGAGCAGTTTGTGGCTGACCCACGACTCAC
  ACTCTGGAGGCAGCAAGGAACAAACATGACAGACAAGTGCAGGCAGCTCTGGGATGAGCTAGGGGCTTTA
  TGGGTGTGCATAATTTTAAATCCACACTGCAAACTGGAGGAGAAATCCTGCTGGCTGCAGCAGCTGCAGA
  AGTGGAGCGACCTGGATGTCTGTCCCCTGGAGGATGGAAACTATGGACATGAGCTGCCCAACATCACCAA
  TGCACTTCCCCAGAGTGCCATTCACAGCCCAGACTCCTTATCCAGACCAAGACGAACAGTATTCACTAGA
  GCCATTGAGGGGCGTGAATTACATTGGCAGGATAGCCACCTACAGCGAATAATCAGCAGTGATGTTTATA
  CAGCTCCTGCCTGCCAGCGGGAAAGTGAAAGACTACTCTTTAATTCCCAAGGCCAGCCACTGTGGCTTGA
  GCATGTGCCTACAGCCTGTGCTCGAGTTGACGCCCTGCGTTCCCATGGTTATCCAAAAGAGGCCCTCAGA
  CTCACAGTGGCCATTATTAATACTCTGAGGTTGCAGCAGCAGCGGCAACTGGAAATCTACAAGCATCAGA
  AGAAAGAACTGTTGCAGAGAGGAACCACAACCATCACAAACCTGGAGGGCTGGGTGGGCCACCCCCTGGA
  TCCCATCGACTGCCTGTTTCTCACTTTGACTGAGGCCTGCCGCCTGAATGATGATGGCTATCTGGAGATG
  TCAGATATGAATGAAAGCAGACCCCCTGTGTACCAGCATGTACCTGTGGCTGCAGGCTCCCCAAACAGCA
  GTGAGTCCTACCTGTCATTGGCCCTGGAAGTTGCACTGATGGGCCTGGGTCAACAGAGGTTAATGCCTGA
  AGGCCTCTACGCACAGGACAAGGTATGCCGTAATGAGGAGCAGCTCCTGAGCCAACTCCAAGAGCTGCAG
  CTGGACGATGAGCTAGTGCAAACACTGCAAAAACAATGCATCTTACTGCTGGAAGGAGGTCCTTTCAGCG
  GTCTGGGAGAAGTCATCCACCGAGAGAGTGTTCCCATGCATACCTTTGCCAAGTATTTGTTCTCAGCTCT
  GCTGCCTCATGATCCAGACCTGTCCTATAAATTAGCCTTACGTGCCATGAGGTTACCTGTTTTAGAAAAC
  TCAGCTTCTGCAGGCGACACATCCCACCCTCACCATATGGTGTCTGTGGTGCCCAGCCGTTATCCTCGCT
  GGTTCACGCTTGGACACTTGGAATCACAGCAGTGTGAACTGGCCTCCACCATGCTGACTGCTGCCAAAGG
  AGACACTTTGAGACTCCGAACAATTCTGGAAGCAATACAGAAGCACATTCATTCTTCCTCCCTCATCTTC
  AAACTGGCCCAAGATGCATTCAAGATTGCTACTCCCACCGACAGTAGTACTGACAGCACCCTGCTCAACG
  TTGCCCTGGAACTTGGGTTACAGGTGATGCGGATGACCTTATCAACCCTTAACTGGAGACGCAGGGAGAT
  GGTACGATGGTTGGTGACCTGTGCTACAGAAGTGGGTGTGCGGGCCCTGGTGAGCATCTTGCAGAGCTGG
  TACACACTCTTCACCCCTACTGAGGCTACTAGTATTGTAGCTGCCACAGCCGTATCCCACACCACTATCC
  TGCGCCTCAGTCTTGACTATCCACAGCGGGAGGAACTGGCTAGCTGTGCTCGCACACTGGCTCTACAGTG
  TGCTATGAAGGATCCACAGAGCTGTGCCCTGTCAGCCCTTACACTCTGTGAGAAAGACCACATTGCCTTT
  GAGGCAGCCTACCAGATTGCCATTGATGCTGCTGCTGGTGGTATGACCCATTCACAGCTGTTCACCATCG
  CCCGCTACATGGAGCTCCGTGGCTACCCGCTACGTGCCTTCAAGCTGGCCTCATTGGCCATGAGCCATCT
  TAACCTGGCCTACAACCAGGATACTCACCCAGCCATCAATGATGTGCTCTGGGCTTGTGCTCTCAGCCAC
  TCTCTGGGCAAGAATGAGCTGGCAGCTCTCATCCCCCTGGTGGTGAAGAGTGTGCACTGTGCCACAGTGC
  TTTCAGACATCTTACGACGCTGCACAGTGACTGCACCTGGCCTGGCCGGCATCCCAGGCCGCCGAAGCTC
  TGGAAAGCTCATGTCCACTGACAAAGCTCCATTGCGCCAGTTGCTGGATGCCACCATCAATGCCTATATC
  AACACTACACACTCACGCCTAACACACATCAGCCCTCGCCACTATGGAGAGTTCATTGAGTTTCTAAGCA
  AGGCTCGGGAGACCTTCCTGCTGCCCCAGGATGGCCACCTGCAGTTTGCCCAGTTCATCGACAACCTCAA
  ACAGATCTACAAAGGCAAGAAAAAGTTGATGCTGCTGGTGCGAGAGCGCTTTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221559 representing NM_020883
  Red=Cloning site Green=Tags(s)
  
  MADGGEREELLSPSPVSPAKRQCSWPSPQAHHPRGSPGAAGGGAGGVGSSCLVLGARPHLQPDSLLDCAA
  KTVAEKWAYERVEERFERIPEPVQRRIVYWSFPRNEREICMYSSFQYRGGPGAGAAGGAAGASPAEEGPQ
  PPPGAAAPAGSAPGGVAAGASPGLGAGAGAAGCGGEGLPFRRGIRLLDSGSVENVLQVGFHLSGTVTELA
  TASEPAVTYKVAISFDRCKITSVTCGCGNKDIFYCAHVVALSLYRIRKPDQVKLRLPISETLFQMNRDQL
  QKFIQYLITAHHTEVLPTAQKLADEILSSNSEINQVNGAPDPTAGASIDDENCWHLDEEQVKEQVKLFLS
  QGGYCGSGKQLNSMFAKVREMLRMRDSNGARMLTLITEQFVADPRLTLWRQQGTNMTDKCRQLWDELGAL
  WVCIILNPHCKLEEKSCWLQQLQKWSDLDVCPLEDGNYGHELPNITNALPQSAIHSPDSLSRPRRTVFTR
  AIEGRELHWQDSHLQRIISSDVYTAPACQRESERLLFNSQGQPLWLEHVPTACARVDALRSHGYPKEALR
  LTVAIINTLRLQQQRQLEIYKHQKKELLQRGTTTITNLEGWVGHPLDPIDCLFLTLTEACRLNDDGYLEM
  SDMNESRPPVYQHVPVAAGSPNSSESYLSLALEVALMGLGQQRLMPEGLYAQDKVCRNEEQLLSQLQELQ
  LDDELVQTLQKQCILLLEGGPFSGLGEVIHRESVPMHTFAKYLFSALLPHDPDLSYKLALRAMRLPVLEN
  SASAGDTSHPHHMVSVVPSRYPRWFTLGHLESQQCELASTMLTAAKGDTLRLRTILEAIQKHIHSSSLIF
  KLAQDAFKIATPTDSSTDSTLLNVALELGLQVMRMTLSTLNWRRREMVRWLVTCATEVGVRALVSILQSW
  YTLFTPTEATSIVAATAVSHTTILRLSLDYPQREELASCARTLALQCAMKDPQSCALSALTLCEKDHIAF
  EAAYQIAIDAAAGGMTHSQLFTIARYMELRGYPLRAFKLASLAMSHLNLAYNQDTHPAINDVLWACALSH
  SLGKNELAALIPLVVKSVHCATVLSDILRRCTVTAPGLAGIPGRRSSGKLMSTDKAPLRQLLDATINAYI
  NTTHSRLTHISPRHYGEFIEFLSKARETFLLPQDGHLQFAQFIDNLKQIYKGKKKLMLLVRERFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020883
• ORF Size: 3555 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020883.2
• RefSeq Size: 5860 bp
• RefSeq ORF: 3558 bp
• Locus ID: 57643
• UniProt ID: Q9P217
• Cytogenetics: 1p34.1
• MW: 130.5 kDa