ZNF827 (NM_178835) Human Tagged ORF Clone

CAT#: RC221405

ZNF827 (Myc-DDK-tagged)-Human zinc finger protein 827 (ZNF827)

Product Images

Circular map for RC221405

• 

  Western blot validation of overexpression lysate (Cat# LY405852) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221405 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF827
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221405 representing NM_178835
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGGAGGAAGCAGGAGCAGCCCAAGCGCCTTCCCTCACATGTTAGTAGGCAGGAAGAGGCGGAGG
  GAGAGCTCAGTGAAGGAGAACACTGGTATGGAAACTCTTCAGAGACTCCGTCAGAAGCATCCTATGGGGA
  AGTCCAGGAGAACTATAAGTTGTCTCTGGAGGACCGGATCCAGGAGCAGTCCACGTCCCCGGACACCTCC
  TTGGGAAGCACGACTCCCAGCAGCCACACGTTGGAGCTGGTGGCACTGGACAGTGAGGTCCTGCGAGACT
  CACTGCAGTGTCAAGATCACCTTTCCCCGGGAGTGTCTTCTTTGTGTGACGATGACCCAGGCTCCAACAA
  GCCCCTGAGCAGCAATTTGAGGCGGCTGCTGGAGGCTGGTTCCCTCAAACTCGATGCTGCAGCCACGGCT
  AATGGCAGAGTGGAGTCCCCCGTAAACGTTGGCTCGAACCTCTCCTTTTCCCCGCCTTCCCACCACGCCC
  AGCAGCTCAGTGTTCTGGCCAGGAAGTTGGCCGAGAAGCAGGAACAGAATGACCAATACACTCCAAGTAA
  CCGTTTTATATGGAATCAAGGTAAGTGGTTGCCAAACTCAACCACCACCTGCAGCTTGTCTCCGGATTCA
  GCCATCCTAAAACTGAAAGCTGCAGCCAATGCCGTTCTGCAGGACAAATCTCTCACCAGGACTGAGGAGA
  CCATGCGATTTGAGTCCTTTTCCTCCCCTTTTAGCTCCCAGTCTGCCAGTTCTACCTTGGCCGCTTTGTC
  CAAGAAGGTCAGTGAAAGAAGTTTGACTCCTGGTCAGGAGCACCCTCCACCGGCCAGCTCCTTCCTTTCC
  CTGGCTTCTATGACCTCCTCAGCGGCCCTTCTGAAGGAGGTGGCCGCAAGGGCTGCGGGCAGTCTTCTGG
  CTGAGAAATCATCGCTGCTGCCTGAGGACCCTCTACCGCCCCCGCCTTCAGAGAAGAAACCAGAAAAAGT
  CACTCCGCCACCTCCACCGCCACCTCCACCACCTCCACCACCACCACCACAATCCCTGGAATTATTATTA
  CTCCCAGTTCCTAAGGGAAGAGTTTCTAAACCCTCCAATTCAGCCTCAGAAGAGGAAAGTGGAAAGCCTT
  TCCAGTGTCCAATATGTGGTTTGGTTATTAAAAGGAAGAGTTACTGGAAGCGGCACATGGTGATTCACAC
  AGGTTTAAAAAGTCATCAGTGTCCGCTCTGTCCATTCCGGTGTGCTCGCAAGGACAATCTCAAATCCCAC
  ATGAAGGTTCATCAGCACCAGGATCGGGGAGAGACCTTTCAGTGCCAGCTGTGCCCTTTTACTTCCTCAC
  GCCACTTCAGCCTGAAACTCCACATGCGTTGCCATCAGCACTTCCTGAGGACAGAAGCCAAGGTGAAGGA
  GGAGATCCCAGACCCAGATGTCAAGGGATCTCCCCACCTCAGTGACAGTGCCTGCCTGGGGCAGCAAAGG
  GAAGGAGGAGGGACAGAGCTAGTGGGGACCATGATGACGTCTAACACTCCAGAGAGGACTAGCCAGGGAG
  GGGCTGGCGTCTCGCCTTTGCTGGTGAAGGAGGAACCCAAGGAAGATAACGGCCTGCCCACCTCCTTTAC
  TTTGAATGCTGCCGACAGGCCCGCCAACCACACAAAGCTGAAAGACCCCTCCGAGTATGTGGCCAACAGT
  GCGTCAGCATTGTTCAGCCAGGACATCTCTGTTAAGATGGCGTCTGATTTTCTCATGAAGCTGTCAGCTG
  CAAATCAGAAGGAGCCCATGAATCTTAATTTTAAAGTGAAAGAGGAGCCTAAGGAAGGGGAGTCCCTAAG
  CACGACTTTGCCTCGGTCCAGCTATGTGTTCAGCCCGGAATCTGAAGTGTCAGCCCCAGGCGTCTCTGAG
  GACGCACTAAAGCCCCAGGAAGGGAAGGGAAGTGTGCTAAGGCGGGATGTGTCAGTCAAAGCAGCCTCTG
  AACTTCTCATGAAACTCTCAGCGGAAAGCTACAAGGAAACACAGATGGTGAAGATTAAAGAGGAACCCAT
  GGAGGTTGACATCCAGGACTCCCATGTCTCGATATCACCCAGCCGGAATGTTGGCTACAGCACTTTAATC
  GGGCGAGAGAAAACCGAACCCTTACAGAAGATGCCAGAGGGCAGAGTACCCCCAGAGAGAAACCTCTTCA
  GTCAGGATATCTCTGTGAAAATGGCTTCCGAGCTCCTCTTTCAACTGTCAGAAAAAGTGAGCAAAGAGCA
  CAATCATACAAAAGAAAACACCATCCGGACCACGACCAGCCCTTTCTTTTCAGAAGACACATTTAGACAA
  TCACCATTCACCTCCAATTCAAAAGAACTGCTGCCCAGTGACTCCGTGCTGCACGGAAGAATATCAGCTC
  CAGAAACAGAAAAGATAGTCCTAGAGGCAGGAAATGGATTACCATCCTGGAAATTCAATGACCAGCTTTT
  TCCCTGTGACGTGTGTGGGAAAGTGTTTGGCCGACAGCAGACATTGTCCCGACACCTCTCGCTGCACACA
  GAGGAAAGAAAATACAAATGCCACTTGTGCCCCTATGCTGCTAAGTGCCGTGCAAATCTGAACCAGCACT
  TGACCGTCCATTCCGTGAAGCTGGTGAGTACAGACACCGAGGACATTGTCAGCGCCGTCACCTCTGAAGG
  CAGTGATGGGAAGAAACATCCTTATTATTACAGTTGTCACGTGTGTGGATTTGAGACCGAGCTCAATGTC
  CAGTTTGTCAGCCACATGTCACTCCACGTGGACAAGGAGCAGTGGATGTTTTCGATCTGCTGCACTGCCT
  GCGACTTCGTCACCATGGAGGAAGCAGAGATAAAGACTCACATTGGCACCAAGCACACAGGGGAAGACAG
  GAAGACCCCCAGCGAATCAAATAGCCCCTCTTCATCCTCCCTCTCAGCTCTGAGTGATTCAGCCAACAGC
  AAAGATGATTCAGATGGCTCCCAGAAAAACAAGGGCGGGAACAATCTGCTGGTCATCTCTGTCATGCCTG
  GGAGCCAGCCCTCACTGAACAGTGAGGAAAAGCCAGAGAAAGGGTTCGAATGTGTTTTTTGCAACTTTGT
  CTGCAAGACGAAGAACATGTTTGAGCGTCATCTGCAGATACACCTCATCACCCGGATGTTTGAGTGTGAT
  GTGTGCCACAAGTTCATGAAGACCCCCGAACAGCTGCTGGAGCATAAGAAATGCCACACTGTCCCCACCG
  GTGGGCTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221405 representing NM_178835
  Red=Cloning site Green=Tags(s)
  
  MPRRKQEQPKRLPSHVSRQEEAEGELSEGEHWYGNSSETPSEASYGEVQENYKLSLEDRIQEQSTSPDTS
  LGSTTPSSHTLELVALDSEVLRDSLQCQDHLSPGVSSLCDDDPGSNKPLSSNLRRLLEAGSLKLDAAATA
  NGRVESPVNVGSNLSFSPPSHHAQQLSVLARKLAEKQEQNDQYTPSNRFIWNQGKWLPNSTTTCSLSPDS
  AILKLKAAANAVLQDKSLTRTEETMRFESFSSPFSSQSASSTLAALSKKVSERSLTPGQEHPPPASSFLS
  LASMTSSAALLKEVAARAAGSLLAEKSSLLPEDPLPPPPSEKKPEKVTPPPPPPPPPPPPPPPQSLELLL
  LPVPKGRVSKPSNSASEEESGKPFQCPICGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSH
  MKVHQHQDRGETFQCQLCPFTSSRHFSLKLHMRCHQHFLRTEAKVKEEIPDPDVKGSPHLSDSACLGQQR
  EGGGTELVGTMMTSNTPERTSQGGAGVSPLLVKEEPKEDNGLPTSFTLNAADRPANHTKLKDPSEYVANS
  ASALFSQDISVKMASDFLMKLSAANQKEPMNLNFKVKEEPKEGESLSTTLPRSSYVFSPESEVSAPGVSE
  DALKPQEGKGSVLRRDVSVKAASELLMKLSAESYKETQMVKIKEEPMEVDIQDSHVSISPSRNVGYSTLI
  GREKTEPLQKMPEGRVPPERNLFSQDISVKMASELLFQLSEKVSKEHNHTKENTIRTTTSPFFSEDTFRQ
  SPFTSNSKELLPSDSVLHGRISAPETEKIVLEAGNGLPSWKFNDQLFPCDVCGKVFGRQQTLSRHLSLHT
  EERKYKCHLCPYAAKCRANLNQHLTVHSVKLVSTDTEDIVSAVTSEGSDGKKHPYYYSCHVCGFETELNV
  QFVSHMSLHVDKEQWMFSICCTACDFVTMEEAEIKTHIGTKHTGEDRKTPSESNSPSSSSLSALSDSANS
  KDDSDGSQKNKGGNNLLVISVMPGSQPSLNSEEKPEKGFECVFCNFVCKTKNMFERHLQIHLITRMFECD
  VCHKFMKTPEQLLEHKKCHTVPTGGLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178835
• ORF Size: 3231 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178835.5
• RefSeq Size: 7814 bp
• RefSeq ORF: 3234 bp
• Locus ID: 152485
• UniProt ID: Q17R98
• Cytogenetics: 4q31.21-q31.22
• Protein Families: Transcription Factors
• MW: 118.7 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]