SEH1L (NM_001013437) Human Tagged ORF Clone

CAT#: RC221329

SEH1L (Myc-DDK-tagged)-Human SEH1-like (S. cerevisiae) (SEH1L), transcript variant 1

Product Images

Circular map for RC221329

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEH1L
• Synonyms: SEC13L; Seh1; SEH1A; SEH1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221329 representing NM_001013437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGTGGCTCGCAGCATCGCGGCGGACCACAAGGATCTCATCCACGATGTCTCTTTCGACTTCCACG
  GGCGGCGGATGGCAACCTGCTCCAGCGATCAGAGCGTTAAGGTCTGGGATAAAAGTGAAAGTGGTGATTG
  GCATTGTACTGCTAGCTGGAAGACACATAGTGGATCTGTATGGCGTGTGACATGGGCCCATCCTGAATTT
  GGGCAGGTTTTGGCTTCCTGTTCTTTTGACCGAACAGCTGCTGTATGGGAAGAAATAGTAGGAGAATCAA
  ATGATAAACTGCGAGGACAGAGCCACTGGGTTAAAAGGACAACTCTGGTGGATAGCAGAACATCTGTTAC
  TGATGTGAAGTTTGCTCCCAAGCACATGGGTCTTATGTTAGCAACCTGTTCCGCAGATGGTATAGTAAGA
  ATCTATGAGGCACCAGATGTTATGAATCTCAGCCAGTGGTCTTTGCAGCATGAGATCTCATGTAAGCTAA
  GCTGTAGTTGTATTTCTTGGAACCCTTCAAGCTCTCGTGCTCATTCCCCCATGATCGCCGTAGGAAGTGA
  TGACAGTAGCCCCAACGCAATGGCCAAGGTTCAGATTTTTGAATATAATGAAAACACCAGGAAATATGCA
  AAAGCTGAAACTCTTATGACAGTCACTGATCCTGTTCATGATATTGCATTCGCTCCAAATTTGGGAAGAT
  CTTTCCATATTCTAGCAATAGCGACCAAAGATGTGAGAATTTTTACATTAAAGCCTGTGAGGAAAGAACT
  GACTTCCTCTGGTGGGCCAACAAAGTTTGAAATCCATATAGTGGCTCAGTTCGATAATCATAATTCTCAG
  GTCTGGCGAGTGAGTTGGAATATAACAGGAACGGTGCTAGCATCTTCAGGAGATGATGGGTGTGTAAGAT
  TGTGGAAAGCTAATTATATGGACAATTGGAAGTGTACTGGTATTTTGAAAGGTAATGGGAGCCCAGTCAA
  TGGGAGTTCTCAGCAGGGAACCTCAAATCCTTCCCTAGGTTCAACTATTCCAAGTCTTCAGAATTCATTA
  AATGGATCTTCTGCTGGCAGGTATTTCTTTACCCCTCTGGATTCCCCACGGGCTGGATCGAGATGGTCCA
  GTTATGCCCAGCTCCTTCCTCCTCCTCCTCCTCCTCTGGTAGAGCACTCTTGCGATGCTGACACTGCCAA
  CCTCCAGTATCCTCACCCTCGCAGACGATATCTCTCTCGGCCTCTTAATCCCTTACCTGAGAATGAAGGG
  ATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221329 representing NM_001013437
  Red=Cloning site Green=Tags(s)
  
  MFVARSIAADHKDLIHDVSFDFHGRRMATCSSDQSVKVWDKSESGDWHCTASWKTHSGSVWRVTWAHPEF
  GQVLASCSFDRTAAVWEEIVGESNDKLRGQSHWVKRTTLVDSRTSVTDVKFAPKHMGLMLATCSADGIVR
  IYEAPDVMNLSQWSLQHEISCKLSCSCISWNPSSSRAHSPMIAVGSDDSSPNAMAKVQIFEYNENTRKYA
  KAETLMTVTDPVHDIAFAPNLGRSFHILAIATKDVRIFTLKPVRKELTSSGGPTKFEIHIVAQFDNHNSQ
  VWRVSWNITGTVLASSGDDGCVRLWKANYMDNWKCTGILKGNGSPVNGSSQQGTSNPSLGSTIPSLQNSL
  NGSSAGRYFFTPLDSPRAGSRWSSYAQLLPPPPPPLVEHSCDADTANLQYPHPRRRYLSRPLNPLPENEG
  I
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013437
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013437.2
• RefSeq Size: 1891 bp
• RefSeq ORF: 1266 bp
• Locus ID: 81929
• UniProt ID: Q96EE3
• Cytogenetics: 18p11.21
• MW: 46.4 kDa
• Gene Summary: The protein encoded by this gene is part of a nuclear pore complex, Nup107-160. This protein contains WD repeats and shares 34% amino acid identity with yeast Seh1 and 30% identity with yeast Sec13. All constituents of the Nup107-160 complex, including this protein, specifically localize to kinetochores in mitosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]