SIX6OS1 (C14orf39) (NM_174978) Human Tagged ORF Clone

CAT#: RC221322

C14orf39 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 39 (C14orf39)

Product Images

Circular map for RC221322

• 

  Western blot validation of overexpression lysate (Cat# LY406369) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221322 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIX6OS1
• Synonyms: Six6os1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221322 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGACAGCCTGTTTGTCAGTTTGGACAGACTTTTGCTAGAATTTGTCTTCCAGTATGAGCAAGACA
  TAAGTACTAAAGAAGAGATGATTCAAAGAATTAATAAATGCTGTGAAGATATTAAGGAAAACAAAGTAAC
  TATTTGTAGGATACACGAAACTATAAATGCAACAGATGAGGAAATTGATCATTACTGTAAACATAGTGAG
  GAGATTAAAGACAACTGTAGAAACTGGAAGCCAACATGTGATGTTTTTCGTAAACATGAAGATTATATGC
  AGGACCAATTTACTGTTTATCAAGGAACTGTTGAAAAAGACAAAGAAATGTATCATGATTATATATGTCA
  GTATAAAGAAGTTTTGAAGCAGTACCAACTAAAATACTCAGAAACACCCTTTTCACGTGAATATTATGAG
  AAGAAAAGAGAACATGAAGAAATTCAAAGCAGAGTGTTGGCATGTACTGAACAATTAAAAATGAATGAAA
  CAATTTTTATGAAATTTCGAGTGCCTGCTCCCTTTCCATCACTTACTAAATGGACTTTAAACATTGTTAA
  TTTGAGATGTGAAACACAAGATATTCTTAAACATGCCAGCAATCTTACCAAAAGTTCATCCGAATTGAAG
  AAAGAAGTAGATGAAATGGAAATAGAAATTAATTATTTAAACCAGCAGATATCTAGGCATAATGAAACTA
  AGGCTCTTTCAGAAACTCTGGAAGAAAAGAACAAAAATACAGAAAACAGAAAAGAACTGAAAGAAAGAAT
  TTTTGGAAAAGATGAGCATGTACTTACATTGAATAAAACTCAAAGCAGTCAATTATTTCTTCCTTATGAA
  TCTCAGAAATTAGTAAGACCAATAAAGATGCATTCTTCAGAACCAAGAGTTGCAGATATAAAAGAAGAAA
  GTTCTGCGAAGCAGTCAAAGCTTGCCAATATTGACTTTAGACAAAAAGAAAATGATACACAGATATTTAA
  TGACTCTGCTGTGGATAACCATTCAAAATGTTCACATATTACGACTATCACAAGTTCACAAAAGTTTATG
  CAAGTCAGATTGTTAACCCCACAGAAACAATCAAATTCCAATCAGTGGTCGGAAAAAGGGGATAAAGATG
  CTGAGTATGGAGATAAAGGGACAGTAAGACAAGTAAGAGAATCAAAATGTACTTCACAAGCTATATATAC
  TGAACATTTTGGGAAGTCAATAGAAAATGATAGTGATGAAGTAGAAGAGAGAGCTGAGAATTTTCCACGA
  ACGTCTGAAATTCCTATATTTTTAGGAACTCCCAAAGCTGTGAAAGCACCTGAGTCATTGGAGAAAATAA
  AATTCCCTAAAACCCCCCCGTTCGAAATTAACAGAAATAGAAATGCAGTACCTGAAGTTCAAACAGAAAA
  GGAATCCCCTGGACTTTCTTTTCTTATGAGTTATACTTCTAGATCACCTGGATTGAATTTATTTGATTCT
  TCTGTATTTGATACAGAAATCTCATCAGATCAGTTTAATGAACATTATTCTGCAAGAAATCTAAATCCTC
  TGTCATCAGAGCAAGAGATTGGAAACTTACTTGAGAAGCCAGAAGGAGAAGATGGCTTTACATTTTCTTT
  TCCATCAGACACTTCAACTCATACATTTGGAGCTGGAAAAGATGATTTTAGTTTTCCATTTTCATTTGGA
  CAGGGTCAAAATTCAATACCTTCTTCTTCTTTAAAAGGTTTTTCATCTTCCTCACAAAATACAACACAGT
  TTACTTTTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221322 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNDSLFVSLDRLLLEFVFQYEQDISTKEEMIQRINKCCEDIKENKVTICRIHETINATDEEIDHYCKHSE
  EIKDNCRNWKPTCDVFRKHEDYMQDQFTVYQGTVEKDKEMYHDYICQYKEVLKQYQLKYSETPFSREYYE
  KKREHEEIQSRVLACTEQLKMNETIFMKFRVPAPFPSLTKWTLNIVNLRCETQDILKHASNLTKSSSELK
  KEVDEMEIEINYLNQQISRHNETKALSETLEEKNKNTENRKELKERIFGKDEHVLTLNKTQSSQLFLPYE
  SQKLVRPIKMHSSEPRVADIKEESSAKQSKLANIDFRQKENDTQIFNDSAVDNHSKCSHITTITSSQKFM
  QVRLLTPQKQSNSNQWSEKGDKDAEYGDKGTVRQVRESKCTSQAIYTEHFGKSIENDSDEVEERAENFPR
  TSEIPIFLGTPKAVKAPESLEKIKFPKTPPFEINRNRNAVPEVQTEKESPGLSFLMSYTSRSPGLNLFDS
  SVFDTEISSDQFNEHYSARNLNPLSSEQEIGNLLEKPEGEDGFTFSFPSDTSTHTFGAGKDDFSFPFSFG
  QGQNSIPSSSLKGFSSSSQNTTQFTFF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174978
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174978.2, NP_777638.2
• RefSeq Size: 2779 bp
• RefSeq ORF: 1764 bp
• Locus ID: 317761
• UniProt ID: Q8N1H7
• Cytogenetics: 14q23.1
• MW: 68.2 kDa
• Gene Summary: Meiotic protein that localizes to the central element of the synaptonemal complex and is required for chromosome synapsis during meiotic recombination. Required for the appropriate processing of intermediate recombination nodules before crossover formation.[UniProtKB/Swiss-Prot Function]