RANK (TNFRSF11A) (NM_003839) Human Tagged ORF Clone

CAT#: RC221263

TNFRSF11A (Myc-DDK-tagged)-Human tumor necrosis factor receptor superfamily, member 11a, NFKB activator (TNFRSF11A)

Product Images

Circular map for RC221263

• 

  Western blot validation of overexpression lysate (Cat# LY418399) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221263 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RANK
• Synonyms: CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCE-R; TRANCER
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221263 representing NM_003839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGCGCGCCCGGCGGCGCCGCCCGCTGTTCGCGCTGCTGCTGCTCTGCGCGCTGCTCGCCCGGC
  TGCAGGTGGCTTTGCAGATCGCTCCTCCATGTACCAGTGAGAAGCATTATGAGCATCTGGGACGGTGCTG
  TAACAAATGTGAACCAGGAAAGTACATGTCTTCTAAATGCACTACTACCTCTGACAGTGTATGTCTGCCC
  TGTGGCCCGGATGAATACTTGGATAGCTGGAATGAAGAAGATAAATGCTTGCTGCATAAAGTTTGTGATA
  CAGGCAAGGCCCTGGTGGCCGTGGTCGCCGGCAACAGCACGACCCCCCGGCGCTGCGCGTGCACGGCTGG
  GTACCACTGGAGCCAGGACTGCGAGTGCTGCCGCCGCAACACCGAGTGCGCGCCGGGCCTGGGCGCCCAG
  CACCCGTTGCAGCTCAACAAGGACACAGTGTGCAAACCTTGCCTTGCAGGCTACTTCTCTGATGCCTTTT
  CCTCCACGGACAAATGCAGACCCTGGACCAACTGTACCTTCCTTGGAAAGAGAGTAGAACATCATGGGAC
  AGAGAAATCCGATGCGGTTTGCAGTTCTTCTCTGCCAGCTAGAAAACCACCAAATGAACCCCATGTTTAC
  TTGCCCGGTTTAATAATTCTGCTTCTCTTCGCGTCTGTGGCCCTGGTGGCTGCCATCATCTTTGGCGTTT
  GCTATAGGAAAAAAGGGAAAGCACTCACAGCTAATTTGTGGCACTGGATCAATGAGGCTTGTGGCCGCCT
  AAGTGGAGATAAGGAGTCCTCAGGTGACAGTTGTGTCAGTACACACACGGCAAACTTTGGTCAGCAGGGA
  GCATGTGAAGGTGTCTTACTGCTGACTCTGGAGGAGAAGACATTTCCAGAAGATATGTGCTACCCAGATC
  AAGGTGGTGTCTGTCAGGGCACGTGTGTAGGAGGTGGTCCCTACGCACAAGGCGAAGATGCCAGGATGCT
  CTCATTGGTCAGCAAGACCGAGATAGAGGAAGACAGCTTCAGACAGATGCCCACAGAAGATGAATACATG
  GACAGGCCCTCCCAGCCCACAGACCAGTTACTGTTCCTCACTGAGCCTGGAAGCAAATCCACACCTCCTT
  TCTCTGAACCCCTGGAGGTGGGGGAGAATGACAGTTTAAGCCAGTGCTTCACGGGGACACAGAGCACAGT
  GGGTTCAGAAAGCTGCAACTGCACTGAGCCCCTGTGCAGGACTGATTGGACTCCCATGTCCTCTGAAAAC
  TACTTGCAAAAAGAGGTGGACAGTGGCCATTGCCCGCACTGGGCAGCCAGCCCCAGCCCCAACTGGGCAG
  ATGTCTGCACAGGCTGCCGGAACCCTCCTGGGGAGGACTGTGAACCCCTCGTGGGTTCCCCAAAACGTGG
  ACCCTTGCCCCAGTGCGCCTATGGCATGGGCCTTCCCCCTGAAGAAGAAGCCAGCAGGACGGAGGCCAGA
  GACCAGCCCGAGGATGGGGCTGATGGGAGGCTCCCAAGCTCAGCGAGGGCAGGTGCCGGGTCTGGAAGCT
  CCCCTGGTGGCCAGTCCCCTGCATCTGGAAATGTGACTGGAAACAGTAACTCCACGTTCATCTCCAGCGG
  GCAGGTGATGAACTTCAAGGGCGACATCATCGTGGTCTACGTCAGCCAGACCTCGCAGGAGGGCGCGGCG
  GCGGCTGCGGAGCCCATGGGCCGCCCGGTGCAGGAGGAGACCCTGGCGCGCCGAGACTCCTTCGCGGGGA
  ACGGCCCGCGCTTCCCGGACCCGTGCGGCGGCCCCGAGGGGCTGCGGGAGCCGGAGAAGGCCTCGAGGCC
  GGTGCAGGAGCAAGGCGGGGCCAAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221263 representing NM_003839
  Red=Cloning site Green=Tags(s)
  
  MAPRARRRRPLFALLLLCALLARLQVALQIAPPCTSEKHYEHLGRCCNKCEPGKYMSSKCTTTSDSVCLP
  CGPDEYLDSWNEEDKCLLHKVCDTGKALVAVVAGNSTTPRRCACTAGYHWSQDCECCRRNTECAPGLGAQ
  HPLQLNKDTVCKPCLAGYFSDAFSSTDKCRPWTNCTFLGKRVEHHGTEKSDAVCSSSLPARKPPNEPHVY
  LPGLIILLLFASVALVAAIIFGVCYRKKGKALTANLWHWINEACGRLSGDKESSGDSCVSTHTANFGQQG
  ACEGVLLLTLEEKTFPEDMCYPDQGGVCQGTCVGGGPYAQGEDARMLSLVSKTEIEEDSFRQMPTEDEYM
  DRPSQPTDQLLFLTEPGSKSTPPFSEPLEVGENDSLSQCFTGTQSTVGSESCNCTEPLCRTDWTPMSSEN
  YLQKEVDSGHCPHWAASPSPNWADVCTGCRNPPGEDCEPLVGSPKRGPLPQCAYGMGLPPEEEASRTEAR
  DQPEDGADGRLPSSARAGAGSGSSPGGQSPASGNVTGNSNSTFISSGQVMNFKGDIIVVYVSQTSQEGAA
  AAAEPMGRPVQEETLARRDSFAGNGPRFPDPCGGPEGLREPEKASRPVQEQGGAKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003839
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003839.1
• RefSeq Size: 3133 bp
• RefSeq ORF: 1851 bp
• Locus ID: 8792
• UniProt ID: Q9Y6Q6
• Cytogenetics: 18q21.33
• Domains: TNFR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• MW: 66.03 kDa
• Gene Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]