ZNF516 (NM_014643) Human Tagged ORF Clone

CAT#: RC221220

ZNF516 (Myc-DDK-tagged)-Human zinc finger protein 516 (ZNF516)

Product Images

Circular map for RC221220

• 

  Western blot validation of overexpression lysate (Cat# LY415147) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221220 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF516
• Synonyms: HsT287
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221220 representing NM_014643
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGCAACAGAGAGGCCGAGATGGAGCTGAGGCGAGGCCCCAGCCCCACCAGGGCCGGCCGGGGCC
  ACGAGGTGGATGGGGACAAGGCTACCTGCCACACCTGCTGCATCTGCGGCAAGAGCTTCCCCTTCCAGAG
  CTCGCTTTCGCAGCACATGCGCAAGCACACGGGCGAGAAGCCCTACAAGTGTCCCTACTGCGACCACCGG
  GCTTCCCAGAAGGGCAACCTGAAGATTCACATCCGGAGCCACCGCACGGGGACTCTGATTCAGGGACACG
  AGCCGGAGGCGGGCGAGGCGCCGCTGGGTGAGATGCGCGCCTCCGAGGGCCTGGACGCCTGCGCCAGCCC
  CACCAAGAGCGCCTCTGCCTGCAACCGGCTGCTGAACGGGGCCTCGCAGGCCGACGGCGCCAGGGTCCTG
  AACGGGGCCTCGCAGGCCGACAGCGGCAGAGTCCTGCTGCGGAGCAGCAAGAAGGGGGCAGAGGGGTCCG
  CATGCGCCCCGGGGGAGGCCAAGGCAGCGGTCCAGTGCTCCTTCTGCAAGAGCCAGTTCGAGCGTAAGAA
  GGACCTGGAGCTGCACGTGCACCAGGCGCACAAGCCGTTCAAGTGCAGGCTGTGCAGCTACGCGACGCTG
  CGGGAGGAGTCGCTGCTGAGCCACATCGAGAGGGACCACATCACCGCGCAGGGGCCCGGCAGCGGCGAGG
  CCTGCGTGGAGAACGGCAAGCCCGAGCTGAGCCCCGGGGAGTTCCCGTGCGAGGTGTGTGGCCAGGCCTT
  CAGCCAGACCTGGTTCCTGAAGGCGCACATGAAGAAGCACCGGGGCTCCTTCGACCACGGCTGCCACATC
  TGCGGCCGTAGGTTCAAGGAGCCCTGGTTCCTCAAGAACCACATGAAGGCGCACGGCCCCAAGACGGGCA
  GCAAGAACAGGCCCAAGAGTGAGCTGGACCCCATCGCCACCATCAACAACGTGGTCCAGGAGGAGGTGAT
  CGTCGCCGGCCTGAGCCTCTACGAGGTCTGCGCCAAGTGCGGGAACCTGTTTACAAACCTGGACAGCTTG
  AACGCCCACAATGCCATCCACCGCAGAGTCGAGGCCAGCCGCACGCGCGCCCCGGCCGAGGAGGGGGCGG
  AGGGGCCCTCGGACACCAAGCAGTTCTTTCTCCAGTGCCTGAACCTGAGGCCGTCGGCGGCCGGCGACTC
  GTGCCCTGGCACGCAGGCCGGACGGCGGGTGGCTGAGCTGGACCCGGTCAACAGCTACCAGGCCTGGCAG
  CTGGCCACGCGGGGTAAGGTGGCCGAGCCGGCCGAGTACCTCAAGTACGGGGCCTGGGACGAGGCGCTGG
  CCGGGGACGTGGCCTTCGACAAGGACAGGCGCGAGTACGTCCTGGTGAGCCAGGAGAAGCGCAAGCGTGA
  GCAGGATGCACCAGCCGCGCAGGGGCCCCCGCGGAAGCGCGCGAGCGGGCCTGGGGACCCCGCGCCCGCC
  GGCCACCTCGATCCCCGCTCGGCCGCGCGCCCCAACCGCAGGGCCGCAGCCACCACCGGCCAGGGCAAGT
  CCTCCGAGTGCTTCGAGTGCGGCAAGATCTTCCGCACCTATCATCAGATGGTGCTGCACTCACGCGTGCA
  TCGCCGCGCGCGCCGCGAGAGGGACAGTGACGGGGACAGGGCGGCGCGGGCCCGCTGCGGATCACTCAGT
  GAGGGTGACTCGGCCTCCCAGCCCAGCAGCCCTGGCTCCGCCTGTGCCGCTGCTGACTCCCCGGGCTCTG
  GCCTGGCCGACGAGGCTGCCGAAGACAGTGGTGAGGAGGGCGCCCCTGAACCTGCACCAGGGGGACAGCC
  GCGCCGCTGCTGCTTTTCCGAAGAGGTGACTTCGACCGAGCTCTCCAGTGGAGACCAGAGTCACAAGATG
  GGAGATAACGCCTCGGAAAGAGACACCGGCGAGTCCAAGGCAGGGATCGCAGCTTCTGTGTCCATACTTG
  AAAACAGTAGCAGAGAGACTTCTAGAAGGCAAGAGCAGCACAGATTTTCTATGGACTTAAAGATGCCAGC
  ATTTCACCCCAAGCAGGAGGTGCCCGTCCCTGGTGATGGTGTGGAGTTCCCTTCCAGTACGGGAGCGGAG
  GGCCAGACGGGTCACCCTGCAGAAAAGCTGTCCGATTTGCACAACAAGGAACACTCTGGGGGAGGGAAGC
  GGGCGCTGGCCCCAGACCTCATGCCGCTAGATTTAAGTGCGAGGTCGACGCGGGATGACCCCAGCAATAA
  GGAGACGGCCTCCTCCCTGCAGGCGGCTTTAGTCGTTCACCCGTGTCCTTACTGCAGCCACAAGACCTAC
  TACCCCGAGGTCCTGTGGATGCACAAACGCATCTGGCACCGTGTCAGCTGCAACTCCGTGGCTCCCCCGT
  GGATTCAGCCCAATGGTTACAAAAGCATCAGAAGCAATTTGGTTTTCCTTTCCCGGAGCGGACGCACGGG
  CCCCCCGCCTGCCCTCGGTGGCAAAGAATGCCAGCCTTTGCTCCTTGCTCGGTTCACCCGCACTCAGGTG
  CCAGGGGGGATGCCGGGGTCCAAAAGTGGCTCTTCTCCCCTGGGAGTGGTCACAAAAGCCGCTAGCATGC
  CTAAGAATAAGGAGAGCCATTCCGGAGGTCCCTGCGCTCTGTGGGCGCCCGGCCCTGACGGGTATCGACA
  GACCAAACCTTGTCACGGCCAGGAGCCACATGGCGCGGCCACACAGGGGCCCCTGGCCAAGCCCAGGCAG
  GAGGCTAGCTCCAAACCGGTGCCTGCCCCGGGTGGCGGGGGCTTCAGCAGGAGCGCCACCCCTACGCCCA
  CCGTCATCGCCCGGGCTGGCGCGCAGCCCTCGGCCAATAGCAAGCCTGTGGAGAAGTTTGGGGTCCCCCC
  AGCGGGGGCTGGCTTTGCCCCCACAAATAAGCACAGTGCCCCGGACTCCCTGAAAGCCAAATTCAGTGCT
  CAGCCTCAGGGTCCACCTCCTGCAAAGGGCGAAGGGGGCGCTCCTCCTCTACCTCCCCGCGAGCCCCCCT
  CGAAGGCAGCCCAGGAGCTGAGGACTCTGGCCACCTGTGCTGCGGGGTCCAGGGGCGACGCGGCCTTGCA
  GGCCCAGCCCGGCGTGGCTGGGGCGCCCCCCGTCCTACACTCCATCAAACAGGAGCCAGTGGCCGAGGGG
  CATGAGAAGCGCCTGGACATCCTCAACATCTTTAAGACGTACATTCCAAAGGACTTTGCGACCCTCTACC
  AGGGATGGGGTGTCAGCGGCCCTGGGTTGGAGCACAGAGGGACACTCCGGACGCAGGCCCGGCCAGGAGA
  GTTCGTCTGCATCGAGTGCGGAAAGAGCTTCCACCAGCCCGGCCACCTCAGGGCCCACATGCGGGCACAC
  TCAGTGGTGTTTGAGTCCGATGGGCCTCGGGGTTCTGAAGTTCATACCACCTCCGCAGACGCCCCCAAAC
  AAGGGAGAGACCATTCTAACACAGGTACCGTCCAGACAGTGCCTCTGAGAAAGGGAACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221220 representing NM_014643
  Red=Cloning site Green=Tags(s)
  
  MDRNREAEMELRRGPSPTRAGRGHEVDGDKATCHTCCICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHR
  ASQKGNLKIHIRSHRTGTLIQGHEPEAGEAPLGEMRASEGLDACASPTKSASACNRLLNGASQADGARVL
  NGASQADSGRVLLRSSKKGAEGSACAPGEAKAAVQCSFCKSQFERKKDLELHVHQAHKPFKCRLCSYATL
  REESLLSHIERDHITAQGPGSGEACVENGKPELSPGEFPCEVCGQAFSQTWFLKAHMKKHRGSFDHGCHI
  CGRRFKEPWFLKNHMKAHGPKTGSKNRPKSELDPIATINNVVQEEVIVAGLSLYEVCAKCGNLFTNLDSL
  NAHNAIHRRVEASRTRAPAEEGAEGPSDTKQFFLQCLNLRPSAAGDSCPGTQAGRRVAELDPVNSYQAWQ
  LATRGKVAEPAEYLKYGAWDEALAGDVAFDKDRREYVLVSQEKRKREQDAPAAQGPPRKRASGPGDPAPA
  GHLDPRSAARPNRRAAATTGQGKSSECFECGKIFRTYHQMVLHSRVHRRARRERDSDGDRAARARCGSLS
  EGDSASQPSSPGSACAAADSPGSGLADEAAEDSGEEGAPEPAPGGQPRRCCFSEEVTSTELSSGDQSHKM
  GDNASERDTGESKAGIAASVSILENSSRETSRRQEQHRFSMDLKMPAFHPKQEVPVPGDGVEFPSSTGAE
  GQTGHPAEKLSDLHNKEHSGGGKRALAPDLMPLDLSARSTRDDPSNKETASSLQAALVVHPCPYCSHKTY
  YPEVLWMHKRIWHRVSCNSVAPPWIQPNGYKSIRSNLVFLSRSGRTGPPPALGGKECQPLLLARFTRTQV
  PGGMPGSKSGSSPLGVVTKAASMPKNKESHSGGPCALWAPGPDGYRQTKPCHGQEPHGAATQGPLAKPRQ
  EASSKPVPAPGGGGFSRSATPTPTVIARAGAQPSANSKPVEKFGVPPAGAGFAPTNKHSAPDSLKAKFSA
  QPQGPPPAKGEGGAPPLPPREPPSKAAQELRTLATCAAGSRGDAALQAQPGVAGAPPVLHSIKQEPVAEG
  HEKRLDILNIFKTYIPKDFATLYQGWGVSGPGLEHRGTLRTQARPGEFVCIECGKSFHQPGHLRAHMRAH
  SVVFESDGPRGSEVHTTSADAPKQGRDHSNTGTVQTVPLRKGT
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014643
• ORF Size: 3489 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014643.1, NP_055458.1
• RefSeq Size: 6033 bp
• RefSeq ORF: 3492 bp
• Locus ID: 9658
• UniProt ID: Q92618
• Cytogenetics: 18q23
• MW: 124.1 kDa
• Gene Summary: Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This gene encodes a zinc-finger protein, and belongs to the krueppel C2H2-type zinc-finger protein family. It may be involved in transcriptional regulation. [provided by RefSeq, Sep 2012]