CDC14B (NM_001077181) Human Tagged ORF Clone

CAT#: RC221171

CDC14B (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 3

Product Images

Circular map for RC221171

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  Western blot validation of overexpression lysate (Cat# LY421370) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221171 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC14B
• Synonyms: Cdc14B1; Cdc14B2; CDC14B3; hCDC14B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221171 representing NM_001077181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGGAGGGCGCGGGGGCAGCTTTGGTAGCCGAGGTGATCAAAGATCGCCTTTGTTTTGCCATTC
  TCTACAGCAGACCAAAGAGTGCATCAAATGTACATTATTTCAGCATAGATAATGAACTTGAATATGAGAA
  CTTCTACGCAGATTTTGGACCACTCAATCTGGCAATGGTTTACAGATATTGTTGCAAGATCAATAAGAAA
  TTAAAGTCCATTACAATGTTAAGGAAGAAAATTGTTCATTTTACTGGCTCTGATCAGAGAAAACAAGCAA
  ATGCTGCCTTCCTTGTTGGATGCTACATGGTTATATATTTGGGGAGAACCCCAGAAGAAGCATATAGAAT
  ATTAATCTTTGGAGAGACATCCTATATTCCTTTCAGAGATGCTGCCTATGGAAGTTGCAATTTCTACATT
  ACACTTCTTGACTGTTTTCATGCAGTAAAGAAGGCAATGCAGTATGGCTTCCTTAATTTCAACTCATTTA
  ACCTTGATGAATATGAACACTATGAAAAAGCAGAAAATGGAGATTTAAATTGGATAATACCAGACCGATT
  TATTGCCTTCTGTGGACCTCATTCAAGAGCCAGACTTGAAAGTGGTTACCACCAACATTCTCCTGAGACT
  TATATTCAATATTTTAAGAATCACAATGTTACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCCA
  AACGCTTTACGGATGCTGGCTTCGATCACCATGATCTTTTCTTTGCGGATGGCAGCACCCCTACTGATGC
  CATTGTCAAAGAATTCCTAGATATCTGTGAAAATGCTGAGGGTGCCATTGCAGTACATTGCAAAGCTGGC
  CTTGGTCGCACGGGCACTCTGATAGCCTGCTACATCATGAAGCATTACAGGATGACAGCAGCCGAGACCA
  TTGCGTGGGTCAGGATCTGCAGACCTGGCTCGGTGATTGGGCCTCAGCAGCAGTTTTTGGTGATGAAGCA
  AACCAACCTCTGGCTGGAAGGGGACTATTTTCGTCAGAAGTTAAAGGGGCAGGAGAATGGACAACACAGA
  GCAGCCTTCTCCAAACTTCTCTCTGGCGTTGATGACATTTCCATAAATGGGGTCGAGAATCAAGATCAGC
  AAGAACCCGAACCGTACAGTGATGATGACGAAATCAATGGAGTGACACAAGGTGATAGACTTCGGGCCTT
  GAAAAGCAGAAGACAATCCAAAACAAACGCTATTCCTCTCACAGTAATTCTTCAATCCAGTGTTCAGAGC
  TGTAAAACATCTGAACCTAACATTTCTGGCAGTGCAGGCATTACTAAAAGAACCACCAGATCTGCTTCAA
  GGAAAAGCAGTGTTAAAAGTCTCTCCATTTCAAGGACTAAAACAGTCTTGCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221171 representing NM_001077181
  Red=Cloning site Green=Tags(s)
  
  MSREGAGAALVAEVIKDRLCFAILYSRPKSASNVHYFSIDNELEYENFYADFGPLNLAMVYRYCCKINKK
  LKSITMLRKKIVHFTGSDQRKQANAAFLVGCYMVIYLGRTPEEAYRILIFGETSYIPFRDAAYGSCNFYI
  TLLDCFHAVKKAMQYGFLNFNSFNLDEYEHYEKAENGDLNWIIPDRFIAFCGPHSRARLESGYHQHSPET
  YIQYFKNHNVTTIIRLNKRMYDAKRFTDAGFDHHDLFFADGSTPTDAIVKEFLDICENAEGAIAVHCKAG
  LGRTGTLIACYIMKHYRMTAAETIAWVRICRPGSVIGPQQQFLVMKQTNLWLEGDYFRQKLKGQENGQHR
  AAFSKLLSGVDDISINGVENQDQQEPEPYSDDDEINGVTQGDRLRALKSRRQSKTNAIPLTVILQSSVQS
  CKTSEPNISGSAGITKRTTRSASRKSSVKSLSISRTKTVLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077181
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077181.3
• RefSeq Size: 5131 bp
• RefSeq ORF: 1386 bp
• Locus ID: 8555
• UniProt ID: O60729
• Cytogenetics: 9q22.32-q22.33
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle
• MW: 52.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]