Inositol Hexakisphosphate Kinase 2 (IP6K2) (NM_001005909) Human Tagged ORF Clone

CAT#: RC221143

IP6K2 (Myc-DDK-tagged)-Human inositol hexakisphosphate kinase 2 (IP6K2), transcript variant 2

Product Images

Circular map for RC221143

• 

  Western blot validation of overexpression lysate (Cat# LY423667) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221143 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Inositol Hexakisphosphate Kinase 2
• Synonyms: IHPK2; InsP6K2; PIUS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221143 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCAGCCTTCAGGGCCATGGATGTGGAGCCCCGCGCCAAAGGCGTCCTTCTGGAGCCCTTTGTCC
  ACCAGGTCGGGGGGCACTCATGCGTGCTCCGCTTCAATGAGACAACCCTGTGCAAGCCCCTGGTCCCAAG
  GGAACATCAGTTCTACGAGACCCTCCCTGCTGAGATGCGCAAATTCACTCCCCAGTACAAAGGTGTGGTA
  TCTGTGCGCTTTGAAGAAGATGAAGACAGGAACTTGTGTCTAATAGCATATCCATTGAAAGGGGACCATG
  GAATTGTGGACATTGTAGATAATTCAGACTGTGAACCAAAAAGTAAGCTCCTAAGGTGGACAACAAACAA
  AAAACATCATGTCTTAGAAACAGAAAAGACCCCTAAGGACTGGGTGCGTCAGCACCGTAAAGAGGAGAAA
  ATGAAGAGCCATAAGTTAGAAGAAGAATTTGAGTGGCTAAAGAAATCTGAAGTCTTGTACTACACTGTAG
  AGAAGAAGTGGAATATAAGTTCCCAGCTTAAACACTATAACCCTTGGAGCATGAAATGTCACCAGCAACA
  GTTACAGAGAATGAAGGAGAATGCAAAGCATCGGAACCAGTACAAATTTATCTTACTGGAAAACCTGACT
  TCCCGCTACGAGGTGCCTTGTGTCCTTGACCTCAAGATGGGCACACGACAACATGGTGATGATGCTTCAG
  AGGAGAAGGCAGCCAACCAGATCCGAAAATGTCAGCAGAGCACATCTGCAGTCATTGGTGTGCGTGTGTG
  TGGCATGCAGGTGTACCAAGCAGGCAGTGGGCAGCTCATGTTCATGAACAAGTACCATGGACGGAAGCTA
  TCGGTGCAGGGCTTCAAGGAGGCACTTTTCCAGTTCTTCCACAATGGGCGGTACCTGCGCCGTGAACTCC
  TGGGCCCTGTGCTCAAGAAGCTGACTGAGCTCAAGGCAGTGTTGGAGCGACAGGAGTCCTACCGCTTCTA
  CTCAAGCTCCCTGCTGGTCATTTATGATGGCAAGGAGCGGCCCGAAGTGGTCCTGGACTCAGATGCTGAG
  GATTTGGAGGACCTGTCAGAGGAATCAGCTGATGAGTCTGCTGGTGCCTATGCCTACAAACCCATCGGCG
  CCAGCTCTGTAGATGTGCGCATGATCGACTTTGCACACACCACCTGCAGGCTGTATGGCGAGGACACCGT
  GGTGCATGAGGGCCAGGATGCTGGCTATATCTTCGGGCTCCAGAGCCTGATAGACATTGTCACAGAGATA
  AGTGAGGAGAGTGGGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221143 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPAFRAMDVEPRAKGVLLEPFVHQVGGHSCVLRFNETTLCKPLVPREHQFYETLPAEMRKFTPQYKGVV
  SVRFEEDEDRNLCLIAYPLKGDHGIVDIVDNSDCEPKSKLLRWTTNKKHHVLETEKTPKDWVRQHRKEEK
  MKSHKLEEEFEWLKKSEVLYYTVEKKWNISSQLKHYNPWSMKCHQQQLQRMKENAKHRNQYKFILLENLT
  SRYEVPCVLDLKMGTRQHGDDASEEKAANQIRKCQQSTSAVIGVRVCGMQVYQAGSGQLMFMNKYHGRKL
  SVQGFKEALFQFFHNGRYLRRELLGPVLKKLTELKAVLERQESYRFYSSSLLVIYDGKERPEVVLDSDAE
  DLEDLSEESADESAGAYAYKPIGASSVDVRMIDFAHTTCRLYGEDTVVHEGQDAGYIFGLQSLIDIVTEI
  SEESGE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005909
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005909.2, NP_001005909.1
• RefSeq Size: 1810 bp
• RefSeq ORF: 1281 bp
• Locus ID: 51447
• UniProt ID: Q9UHH9
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• MW: 49.3 kDa
• Gene Summary: This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4 and affect the growth suppressive and apoptotic activities of interferon-beta in some ovarian cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]