ADAM2 (NM_001464) Human Tagged ORF Clone

CAT#: RC221139

ADAM2 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 2 (ADAM2)

Product Images

Circular map for RC221139

• 

  Western blot validation of overexpression lysate (Cat# LY419910) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221139 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM2
• Synonyms: CRYN1; CRYN2; CT15; FTNB; PH-30b; PH30; PH30-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221139 representing NM_001464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGCGTCTTGTTTCTGCTCAGCGGGCTCGGCGGGCTGCGGATGGACAGTAATTTTGATAGTTTAC
  CTGTGCAAATTACAGTTCCGGAGAAAATACGGTCAATAATAAAGGAAGGAATTGAATCGCAGGCATCCTA
  CAAAATTGTAATTGAAGGGAAACCATATACTGTGAATTTAATGCAAAAAAACTTTTTACCCCATAATTTT
  AGAGTTTACAGTTATAGTGGCACAGGAATTATGAAACCACTTGACCAAGATTTTCAGAATTTCTGCCACT
  ACCAAGGGTATATTGAAGGTTATCCAAAATCTGTGGTGATGGTTAGCACATGTACTGGACTCAGGGGCGT
  ACTACAGTTTGAAAATGTTAGTTATGGAATAGAACCCCTGGAGTCTTCAGTTGGCTTTGAACATGTAATT
  TACCAAGTAAAACATAAGAAAGCAGATGTTTCCTTATATAATGAGAAGGATATTGAATCAAGAGATCTGT
  CCTTTAAATTACAAAGCGTAGAGCCACAGCAAGATTTTGCAAAGTATATAGAAATGCATGTTATAGTTGA
  AAAACAATTGTATAATCATATGGGGTCTGATACAACTGTTGTCGCTCAAAAAGTTTTCCAGTTGATTGGA
  TTGACGAATGCTATTTTTGTTTCATTTAATATTACAATTATTCTGTCTTCATTGGAGCTTTGGATAGATG
  AAAATAAAATTGCAACCACTGGAGAAGCTAATGAGTTATTACACACATTTTTAAGATGGAAAACATCTTA
  TCTTGTTTTACGTCCTCATGATGTGGCATTTTTACTTGTTTACAGAGAAAAGTCAAATTATGTTGGTGCA
  ACCTTTCAAGGGAAGATGTGTGATGCAAACTATGCAGGAGGTGTTGTTCTGCACCCCAGAACCATAAGTC
  TGGAATCACTTGCAGTTATTTTAGCTCAATTATTGAGCCTTAGTATGGGGATCACTTATGATGACATTAA
  CAAATGCCAGTGCTCAGGAGCTGTCTGCATTATGAATCCAGAAGCAATTCATTTCAGTGGTGTGAAGATC
  TTTAGTAACTGCAGCTTCGAAGACTTTGCACATTTTATTTCAAAGCAGAAGTCCCAGTGTCTTCACAATC
  AGCCTCGCTTAGATCCTTTTTTCAAACAGCAAGCAGTGTGTGGTAATGCAAAGCTGGAAGCAGGAGAGGA
  GTGTGACTGTGGGACTGAACAGGATTGTGCCCTTATTGGAGAAACATGCTGTGATATTGCCACATGTAGA
  TTTAAAGCCGGTTCAAACTGTGCTGAAGGACCATGCTGCGAAAACTGTCTATTTATGTCAAAAGAAAGAA
  TGTGTAGGCCTTCCTTTGAAGAATGCGACCTCCCTGAATATTGCAATGGATCATCTGCATCATGCCCAGA
  AAACCACTATGTTCAGACTGGGCATCCGTGTGGACTGAATCAATGGATCTGTATAGATGGAGTTTGTATG
  AGTGGGGATAAACAATGTACAGACACATTTGGCAAAGAAGTAGAGTTTGGCCCTTCAGAATGTTATTCTC
  ACCTTAATTCAAAGACTGATGTATCTGGAAACTGTGGTATAAGTGATTCAGGATACACACAGTGTGAAGC
  TGACAATCTGCAGTGCGGAAAATTAATATGTAAATATGTAGGTAAATTTTTATTACAAATTCCAAGAGCC
  ACTATTATTTATGCCAACATAAGTGGACATCTCTGCATTGCTGTGGAATTTGCCAGTGATCATGCAGACA
  GCCAAAAGATGTGGATAAAAGATGGAACTTCTTGTGGTTCAAATAAGGTTTGCAGGAATCAAAGATGTGT
  GAGTTCTTCATACTTGGGTTATGATTGTACTACTGACAAATGCAATGATAGAGGTGTATGCAATAACAAA
  AAGCACTGTCACTGTAGTGCTTCATATTTACCTCCAGATTGCTCAGTTCAATCAGATCTATGGCCTGGTG
  GGAGTATTGACAGTGGCAATTTTCCACCTGTAGCTATACCAGCCAGACTCCCTGAAAGGCGCTACATTGA
  GAACATTTACCATTCCAAACCAATGAGATGGCCATTTTTCTTATTCATTCCTTTCTTTATTATTTTCTGT
  GTACTGATTGCTATAATGGTGAAAGTTAATTTCCAAAGGAAAAAATGGAGAACTGAGGACTATTCAAGCG
  ATGAGCAACCTGAAAGTGAGAGTGAACCTAAAGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221139 representing NM_001464
  Red=Cloning site Green=Tags(s)
  
  MWRVLFLLSGLGGLRMDSNFDSLPVQITVPEKIRSIIKEGIESQASYKIVIEGKPYTVNLMQKNFLPHNF
  RVYSYSGTGIMKPLDQDFQNFCHYQGYIEGYPKSVVMVSTCTGLRGVLQFENVSYGIEPLESSVGFEHVI
  YQVKHKKADVSLYNEKDIESRDLSFKLQSVEPQQDFAKYIEMHVIVEKQLYNHMGSDTTVVAQKVFQLIG
  LTNAIFVSFNITIILSSLELWIDENKIATTGEANELLHTFLRWKTSYLVLRPHDVAFLLVYREKSNYVGA
  TFQGKMCDANYAGGVVLHPRTISLESLAVILAQLLSLSMGITYDDINKCQCSGAVCIMNPEAIHFSGVKI
  FSNCSFEDFAHFISKQKSQCLHNQPRLDPFFKQQAVCGNAKLEAGEECDCGTEQDCALIGETCCDIATCR
  FKAGSNCAEGPCCENCLFMSKERMCRPSFEECDLPEYCNGSSASCPENHYVQTGHPCGLNQWICIDGVCM
  SGDKQCTDTFGKEVEFGPSECYSHLNSKTDVSGNCGISDSGYTQCEADNLQCGKLICKYVGKFLLQIPRA
  TIIYANISGHLCIAVEFASDHADSQKMWIKDGTSCGSNKVCRNQRCVSSSYLGYDCTTDKCNDRGVCNNK
  KHCHCSASYLPPDCSVQSDLWPGGSIDSGNFPPVAIPARLPERRYIENIYHSKPMRWPFFLFIPFFIIFC
  VLIAIMVKVNFQRKKWRTEDYSSDEQPESESEPKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001464
• ORF Size: 2205 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001464.5
• RefSeq Size: 2657 bp
• RefSeq ORF: 2208 bp
• Locus ID: 2515
• UniProt ID: Q99965
• Cytogenetics: 8p11.22
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 82.3 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded protein is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]