WDR74 (NM_018093) Human Tagged ORF Clone

CAT#: RC221134

WDR74 (Myc-DDK-tagged)-Human WD repeat domain 74 (WDR74)

Product Images

Circular map for RC221134

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY WDR74 (Cat# RC221134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR74(Cat# TA800467). Positive lysates LY413318 (100ug) and LC413318 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413318) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221134 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR74
• Synonyms: Nsa1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221134 representing NM_018093
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCTGCTGCACGCTGGAACCATGTGTGGGTCGGCACCGAGACTGGGATCTTGAAAGGGGTAA
  ATCTTCAGCGAAAACAGGCGGCGAACTTCACGGCCGGAGGACAGCCGCGGCGCGAGGAGGCAGTGAGCGC
  CCTGTGTTGGGGCACCGGCGGCGAGACCCAGATGCTGGTGGGCTGCGCGGACAGGACGGTGAAGCACTTC
  AGCACCGAGGATGGCATATTCCAGGGTCAGAGACACTGCCCGGGCGGGGAGGGCATGTTCCGTGGCCTCG
  CCCAGGCCGACGGCACCCTCATCACATGTGTGGATTCTGGGATTCTCAGAGTCTGGCATGACAAGGACAA
  GGACACATCCTCTGACCCACTCCTGGAACTGAGAGTGGGCCCTGGGGTGTGTAGGATGCGCCAAGACCCA
  GCACACCCCCATGTGGTTGCCACAGGTGGGAAAGAGAATGCTTTGAAGATATGGGACCTGCAGGGCTCTG
  AGGAACCTGTGTTCAGGGCCAAGAACGTGCGGAATGACTGGCTGGACCTGCGGGTTCCCATCTGGGACCA
  GGACATACAGTTTCTCCCAGGATCACAGAAGCTTGTCACCTGCACAGGGTACCACCAGGTCCGTGTTTAT
  GATCCAGCATCCCCCCAGCGCCGGCCAGTCCTAGAGACCACCTATGGAGAGTACCCACTAACAGCCATGA
  CCCTCACTCCGGGAGGCAACTCAGTGATTGTGGGAAACACTCATGGGCAGCTGGCAGAAATTGACCTTCG
  GCAAGGGCGTCTACTGGGCTGTCTGAAGGGGCTGGCAGGCAGTGTGCGTGGGTTGCAGTGCCACCCTTCA
  AAGCCTCTACTAGCCTCCTGTGGCTTGGACAGAGTCTTGAGGATACACAGGATCCAGAATCCACGGGGTC
  TGGAGCATAAGGTTTATCTCAAGTCTCAATTGAACTGCCTCCTCTTGTCAGGCAGGGACAACTGGGAGGA
  TGAGCCCCAAGAGCCTCAAGAACCCAACAAGGTGCCCCTAGAAGACACAGAGACAGATGAACTTTGGGCA
  TCCTTGGAGGCAGCTGCCAAGCGGAAGCTCTCGGGTTTGGAGCAGCCCCAAGGAGCTCTCCAAACGAGAC
  GGAGAAAGAAGAAGCGGCCTGGGTCCACCAGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221134 representing NM_018093
  Red=Cloning site Green=Tags(s)
  
  MAAAAARWNHVWVGTETGILKGVNLQRKQAANFTAGGQPRREEAVSALCWGTGGETQMLVGCADRTVKHF
  STEDGIFQGQRHCPGGEGMFRGLAQADGTLITCVDSGILRVWHDKDKDTSSDPLLELRVGPGVCRMRQDP
  AHPHVVATGGKENALKIWDLQGSEEPVFRAKNVRNDWLDLRVPIWDQDIQFLPGSQKLVTCTGYHQVRVY
  DPASPQRRPVLETTYGEYPLTAMTLTPGGNSVIVGNTHGQLAEIDLRQGRLLGCLKGLAGSVRGLQCHPS
  KPLLASCGLDRVLRIHRIQNPRGLEHKVYLKSQLNCLLLSGRDNWEDEPQEPQEPNKVPLEDTETDELWA
  SLEAAAKRKLSGLEQPQGALQTRRRKKKRPGSTSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018093
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018093.3
• RefSeq Size: 1366 bp
• RefSeq ORF: 1158 bp
• Locus ID: 54663
• UniProt ID: Q6RFH5
• Cytogenetics: 11q12.3
• Domains: WD40
• MW: 42.3 kDa
• Gene Summary: Regulatory protein of the MTREX-exosome complex involved in the synthesis of the 60S ribosomal subunit (PubMed:26456651). Participates in an early cleavage of the pre-rRNA processing pathway in cooperation with NVL (PubMed:29107693). Required for blastocyst formation, is necessary for RNA transcription, processing and/or stability during preimplantation development (By similarity).[UniProtKB/Swiss-Prot Function]