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C2CD3 (NM_015531) Human Tagged ORF Clone
CAT#: RC220925
- TrueORF®
C2CD3 (Myc-DDK-tagged)-Human C2 calcium-dependent domain containing 3 (C2CD3)
ORF Plasmid: tGFP
"NM_015531" in other vectors (2)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | C2CD3 |
| Synonyms | OFD14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220925 representing NM_015531
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACAACGAAAAGGCCAAGGGTCTGGGGGCAGCCGTGGGCGCAAAAAAAGAGGTTTAAGTGACATTT CTCCATCTACAAGCCTGCCACCTCTGGTTGAAGGCCAGCTACGCTGTTTTCTAAAACTTACTGTTAATAG AGTCATATGGAAGATTGCAAAGCCTCCCACTTGTGTACTTGTCCGAGTGAGATGGTGGGGAGAAACATCA GATGGAACCCTCTTTTGTCCCAGGGATGCATTGCAGACTGAACCAAAAGCTGTGAGAACAACTACGCGTT ACGCTATTCGTTGTGGTCCAAAACAGTTTACCTCTTATCTAACAGATATGGCTGTGCTGGTGCTGGAAGT AATCACCAAACTTGATGGTCTTCCAATTGGTAGAGTTCAGATCAATGGACTAGCTCAACTTTCTCCAACC CATCAAACCAATGGATTTTTTACCATTGTTTCATCAACGTCTAAGAAACTTGGAGAACTCCAGGTCTCAC TTGCCCTGGAACCTCTGTCAGAAACTTACGACAGCTACCATCCACTTCCTACCACTGACATGACAGAAAA TGTGCTTTTATCTAAGCAGGGATTCAGAGAGAATACTGAACCCAGCAGTACCCAGTTTCAGGTTCCATCA AGGCCTCGCGACATACATACCATCAAAATTGATGGAAAAGAGTTAGCAGCCAACAGCAGTAGATCAACCA CTCCGAGGGGAAAAGACCATGTATGCTTTGCAGAGAACCCTGATACAATAAAGGATTCTTCCTTTGGACT ACAGCACAGTCTTAATTCAGGACAGAGTTTAGAGTCTGTAACTCTGAAAGGCAGAGCTCCACGGAAGCAG ATGTCCCTTCTGAACAGTTCTGAATTCCAGCCTCAAATTAGAACAGTTGCCAAGAGTCACAGTGACTCAT GCATTCTTTCTTCAAACAACCTCCCTACCAAGGATCTTCTTTCAGCTCTGTTAGAACAAGGCAATAAACT GCGTAATGCCATGGTGATTTCTGCAATGAAATCAAGCCCAGAGACCAGCATGTTGTTGGACCAAGTTCAT CCTCCTATTAATGAAGATTCTCTTAGAGCATCAACACAGATCAGAGCCTTTTCTAGGAATCGGTTTAAAG ACCACATTGAAGATCACCTCCTCCCTTCAACTGAGAATACATTTTGGAGACATGACACAAAAGCTGATAC CAGAGCTATACAGCTGCTATTAGGCAGTGCTGAATTATCCCAAGGCAATTTCTGGGATGGGCTAGGCTCT CCTCCAGATTCCCCATCTCCTGGGAGTGATGTGTATTGCATCAGTGAGCTGAATGACCCTCAGTATGACC AGAGTCTTCTGGAGAATTTATTTTATACAGCACCTAAATCTGATACCAGCATCAGTGATTTCCTCAGTGA AGAGGATGATATCGTCCCTTCTAAAAAAATAAGCCAGTCAACAGCTCTTGCCAGATCATCTAAGGTTCTG GAGTCAAGTGATCATAAGCTGAAGAAGAGGTCAGCAGGCAAGAGAAATAGAAATTTGGTTGAACAACAGA TGCTCTCAGAAACTCCAGAAGATGCCCAAACGATGACACTAAGTGTGGATAGACTGGCCCTTTTGGGTAG AACACATTCAGTCAGAATCATCATCGAAACCATGGGAGTTCCTCCAGATAGTCCTCAGATGACCCCTGGC AAAAAAAGCTATGCTGGTCCACCACCTAAGGTGACTACAGCAAAAAAGCGCACTTTCTTTGTAGAATATC ACTTTCCTGTGGGCTTTTCGGAAAGCGGATTGGGAAAGACAGCTTTGATCACTGAGGTTGTTCGACTCGC CTCCAGTAAAATTACAGATGGAAAGGTGAAGTTCCAGCAGCGATTTGTGTTTCCAGTACAGTTTGGTGGC CCAATGATAGAGCACTGGTGGAATTCCAACCTCACTTTCCAAATTTATGTAAAGAAAACTCCACAGAAAA AGCCAGAAGTCATTGGATCTGTGTCACTTTCTTTGCGAGCTGTCATTCAATCAGAGCTGCTTTCTTTCAG TGATCAGCTTCCAGTGCAACAAGAAAATGGTCAGTCTCCATTTGGCCCCCTCAAGGTAACCATGGAGCTT ATTACAGATAACAAAGATTTCACTGGTATCAATACTAAGTTAAGTGGCAACACCCATTATACCCCACTTT GTGCTCCTACAAGTCCAAATAAGGCACTACCAGAACTTAACCAAGATGTGACCTGTACCAAAAATCCACA AAACTTAAACCAAATTCATGAGGAAACTGCAAAGAAAGCACAGAACTTGGTGCTCCCCAACCGAAAGTCA CCAAGCCCTGTAGCACCACATCCTTCAACCTTCGTAGCTACGCCAGCCTCCCATAATTTAGTCAATCAGA CAAATGGGACAACAAAAGAGAGTGCTTTGCTGTTGCATGTGCTGTTGATGGTGCCAGATGGGAAAGATTT TATTAGTGGAGAATCTGAGAAACAATCACCATGCAATGTTTATTTAAATTGTAAACTCTTCAGCACAGAG GAAGTCACCAGATCTGTCATCGCATGGGGCACAACACAACCGGTCTTTAACTTTTCTCAGGTGATTCCTG TCTCTCTGTCTTCCAAATACCTGGAAAGGCTTAAGAACAATGTGATGGTAATTGAAACTTGGAATAAGGT GCGGAGCCCAGGACAGGACAAGCTGCTCGGGCTGGTGAAACTTCCCCTCCACCAGTTTTACATGTCATTC AAAGATGCTAAGATTTCTCGCCTGTTGCTGGATGCCCAGTACCCAGTTGTTGCTGTCGACAGCTACATGC CTGTGATTGATGTGTTTTCAGGCCACCAAAATGGGAGTCTTCGAGTCTTTTTAGCTATGGGTTCTTCAAA TCAAATAATGGCACTACAAAGATTAAAGAATGAAGAAGGAACACTCCCTCCCTTCAGCCCTAGGCCAGCC CATTTCCTGGACCAGCCAACTGCAGCATCTGTTGCTATGGCAGAGGACCGAGGAAATGGACTGATGGAGC ACTGCTTTGAGATCCATATAGAGATGGTTAAAGGGCTAGCCCCTCTTCAGGCAACAGTCTGGGGAGAAGC AGATTGTTATGTCCAGTACTACTTTCCAGTTCAACACTCTCAATCCAGTGTGCTGAAAGGACCTGAGTTC CTTGAAAATGGAATTACTCTGAAGCCCTTCAGAACTGCAACCACACTCTGTGTTCCAGATCCCATCTTTA ATAGTGAACACCATCACTCTCTCCTGTTGCCAGCTGAGGTTCCAGTGCAAAGGCTCCTACTAAGTGCTTT CTCTGCACAGGGCCTCGTGCCTGGAGGTGGAGTCCAGTTTGAAATCTGGTGCAGATACTATTATCCTAAT GTGAGAGACCAGAAGGTCGCCAAAGGAACCTTGCCATTATCAAGGATCTGTGCTATGGTAGCCACCCAGC ATCGTGAGGATGTGGGAATACAGACCTTTAATCTCCCTTTAACCCCCAGGATTGAGAACAGGAAAGAATT GAGGAACCAGTCATCAGGTTTACTGGATGTGGGCCTAAGGTACAGGCGTAGTCCAAGAACAGCAGAGGGA GTTCTTGCTGCCCGAACCGTTTCCATCTCAGTCCAGATTATCAGAGCCTGTGGTCTGCAAGCAGCAGCCA AGGCTTTGGCTGAACAGGAACCCGCTCTACAGTTTAGTGCCACAGTCGGGGTCAATGCCTCTGTCACCAC TCATCTCTCCTTCCTGCCCCAGGGAGAACAGCGCCGAACCCACCCTGTGGCCTGTTCTTTCTGCCCTGAG TTCTCCCATCACGTTGAGTTCACATGTAACTTGGTGACTCAGCACTGTAGTGGAGAGGCCTGTTTCCTAG CAGAGTTGTTGGAGTTTGCAGAAGTTATTTTTGCTGTCTATCATGAAAATACCAAGTCAGCAAGTGATAT AATCAGTATTGAGTCATGCAAAGAGTATCTGCTTGGAGTAGTAAAAGTTCCAACAAAAGAGCTGCTGATC AAGAGATCTGGGATCACAGGATGGTATCCTATCATTTTACCAGAAGACGGGGGCCTACCTCGTGGCCTGG AGCTCATGCAGAAGATCGTGGGTGGTCTGGAGCTTTCGATTTCCTTCACGCATCGTGGAGATAGAGAACG GGTGTTGGAAGCTGCTGAGCATTTGGGCTGGAGCTTTGAGAACAGCCTGAAAGATTTTGTCAGAATGGAT GAAGGGGAGCCAGCCACTGTCACCATCTCCACCCCAAGGCTGTGGCTGTCCATCCATTGTGTGCTGCTTG CTGGCCACAACCACATTCATAAGAATACATATTGCTACCTTCGCTACAAGTTCTATGATCATGAAGCCTT TTGGACCCCTCTCAAGAAGCCTAAGGAATCTGTAAACAAAAAGCAGATTATGGTCACTTTCAAGGCATCC AAAAGAGCAGAAGTCACCAGGGGCCCATCACTGCTTTGGTACTTCAGGGAGGAGAGGCTAGAGATCCAAG TGTGGCGAGCTTATGGCAATGACAGTGTGGAGAGACCCCATCAGACAGACAGCTGGATTGGCTCAGCCTA TGTGGACCTGGCCAGACTTGGGGAGAGGTCAGCGAGGACGCTAACTGTCAGTGGTGTGTATCCTCTGTTT GGACGAAATGCTTCCAACCTCTCAGGAGCTGCCTTGCGAGTTCATGTGGTTCTTTCCTCTCTTTCCTCAC ACCTTGAGCCCACTCATGAGCTGGACTCCATGGACTGCAGCAGCCACAGTGAGTCTGAGCAGCTCCCCAG AAGGAATGATGAGGTCCAGCTCTCTCCACCAGAAGTCATCTCCTGCCACCAGAAGTCTCCTGCCTCCACC CAGGTCCCCTGCAGCAGCACCACAGCTGAAGTCCGCCTGACGCAGGAGGGCCCTGCTGATTTGGATGGAA CGTTTGCAGTCAGCATCCTAGTGGAAAGAGCAATGCACTTGAGCTTGAAAGGGAGCCCCTTGACAGAGCG GAAAGTATCGATACCCAGTTGTTGTGTATCCTTTGCAACAGCCGATGAGTCATCTCCTGTATACACCCAA GTGGTTGAAAACACAGATTCCCCCATCTGGAATTTTCAACAGCAGTCAAGGCTATCAAAAGAGCTGCTTC TGGACCCACAACAAACCCTGGTCTTCAAAGTTTGGCATAAAGGAGATGAGGAGAGGGTGATTGGCTTTGC CTCGGTGGACCTCTCCCCACTTCTCTCTGGCTTCCAGTTTGTCTGTGGCTGGTACAACATCACAGACTTC AGTGGAGAGTGCCAGGGGCAGATAAAAGTTGCTGTCTCCCCTTTGGAGAGTTTGATACACTTCAAAGAAG AAAGGCAAGCAAGGCGTGGAGTGGAGACCTCAAAATCACTGATCCCAATATACAGTCCCTTTTCCTTCCC TGCCTCTGATACGTATGCTGCATTCTCCAGCCACATGGCAAGGCAGACCCTAGACCAACTTGCTCATGCC TCCTCAAAGGAGCTTGATTTCTCCTCTCCTGGGAGAAGTGATACCACAAGAAGCCAAGCATCACGCCATG AAGAGCATGTGCAGAACATTCGCCGGTTTCATGAATCCCTGCATCTTCAGGGAGAGGCACCCTTGCCATG TGATGACAAACTGACCACATCACCTTTGTCCTCCCAAACCTCCATTCTGACTTCTCTCAGGAAGAATCTG AGTGAGCTTGATCAGATTCAGAGGTACTTCCGCCAGAAGCTCACCAAGCCTTTCCTACCCCTCAGCCCTC AGACTCAAACGGCCATCTCACAGCACCAGGAGAGCTGTAGGGACCATCTTGGGCCAGGTGCCAGCAGCCT AGACCCTGGGAGCCAGTGTATCCTGGAGAAATCCAGTAACCTGGTGTTGCAAGTCAGCTCCTTAATCACA GGTAGTTAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC220925 representing NM_015531
Red=Cloning site Green=Tags(s) MKQRKGQGSGGSRGRKKRGLSDISPSTSLPPLVEGQLRCFLKLTVNRVIWKIAKPPTCVLVRVRWWGETS DGTLFCPRDALQTEPKAVRTTTRYAIRCGPKQFTSYLTDMAVLVLEVITKLDGLPIGRVQINGLAQLSPT HQTNGFFTIVSSTSKKLGELQVSLALEPLSETYDSYHPLPTTDMTENVLLSKQGFRENTEPSSTQFQVPS RPRDIHTIKIDGKELAANSSRSTTPRGKDHVCFAENPDTIKDSSFGLQHSLNSGQSLESVTLKGRAPRKQ MSLLNSSEFQPQIRTVAKSHSDSCILSSNNLPTKDLLSALLEQGNKLRNAMVISAMKSSPETSMLLDQVH PPINEDSLRASTQIRAFSRNRFKDHIEDHLLPSTENTFWRHDTKADTRAIQLLLGSAELSQGNFWDGLGS PPDSPSPGSDVYCISELNDPQYDQSLLENLFYTAPKSDTSISDFLSEEDDIVPSKKISQSTALARSSKVL ESSDHKLKKRSAGKRNRNLVEQQMLSETPEDAQTMTLSVDRLALLGRTHSVRIIIETMGVPPDSPQMTPG KKSYAGPPPKVTTAKKRTFFVEYHFPVGFSESGLGKTALITEVVRLASSKITDGKVKFQQRFVFPVQFGG PMIEHWWNSNLTFQIYVKKTPQKKPEVIGSVSLSLRAVIQSELLSFSDQLPVQQENGQSPFGPLKVTMEL ITDNKDFTGINTKLSGNTHYTPLCAPTSPNKALPELNQDVTCTKNPQNLNQIHEETAKKAQNLVLPNRKS PSPVAPHPSTFVATPASHNLVNQTNGTTKESALLLHVLLMVPDGKDFISGESEKQSPCNVYLNCKLFSTE EVTRSVIAWGTTQPVFNFSQVIPVSLSSKYLERLKNNVMVIETWNKVRSPGQDKLLGLVKLPLHQFYMSF KDAKISRLLLDAQYPVVAVDSYMPVIDVFSGHQNGSLRVFLAMGSSNQIMALQRLKNEEGTLPPFSPRPA HFLDQPTAASVAMAEDRGNGLMEHCFEIHIEMVKGLAPLQATVWGEADCYVQYYFPVQHSQSSVLKGPEF LENGITLKPFRTATTLCVPDPIFNSEHHHSLLLPAEVPVQRLLLSAFSAQGLVPGGGVQFEIWCRYYYPN VRDQKVAKGTLPLSRICAMVATQHREDVGIQTFNLPLTPRIENRKELRNQSSGLLDVGLRYRRSPRTAEG VLAARTVSISVQIIRACGLQAAAKALAEQEPALQFSATVGVNASVTTHLSFLPQGEQRRTHPVACSFCPE FSHHVEFTCNLVTQHCSGEACFLAELLEFAEVIFAVYHENTKSASDIISIESCKEYLLGVVKVPTKELLI KRSGITGWYPIILPEDGGLPRGLELMQKIVGGLELSISFTHRGDRERVLEAAEHLGWSFENSLKDFVRMD EGEPATVTISTPRLWLSIHCVLLAGHNHIHKNTYCYLRYKFYDHEAFWTPLKKPKESVNKKQIMVTFKAS KRAEVTRGPSLLWYFREERLEIQVWRAYGNDSVERPHQTDSWIGSAYVDLARLGERSARTLTVSGVYPLF GRNASNLSGAALRVHVVLSSLSSHLEPTHELDSMDCSSHSESEQLPRRNDEVQLSPPEVISCHQKSPAST QVPCSSTTAEVRLTQEGPADLDGTFAVSILVERAMHLSLKGSPLTERKVSIPSCCVSFATADESSPVYTQ VVENTDSPIWNFQQQSRLSKELLLDPQQTLVFKVWHKGDEERVIGFASVDLSPLLSGFQFVCGWYNITDF SGECQGQIKVAVSPLESLIHFKEERQARRGVETSKSLIPIYSPFSFPASDTYAAFSSHMARQTLDQLAHA SSKELDFSSPGRSDTTRSQASRHEEHVQNIRRFHESLHLQGEAPLPCDDKLTTSPLSSQTSILTSLRKNL SELDQIQRYFRQKLTKPFLPLSPQTQTAISQHQESCRDHLGPGASSLDPGSQCILEKSSNLVLQVSSLIT GSY SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015531 |
| ORF Size | 5889 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015531.2, NP_056346.2 |
| RefSeq Size | 6036 bp |
| RefSeq ORF | 5892 bp |
| Locus ID | 26005 |
| UniProt ID | Q4AC94 |
| Cytogenetics | 11q13.4 |
| MW | 217.9 kDa |
| Gene Summary | This gene encodes a protein that functions as a regulator of centriole elongation. Studies of the orthologous mouse protein show that it promotes centriolar distal appendage assembly and is also required for the recruitment of other ciliogenic proteins, including intraflagellar transport proteins. Mutations in this gene cause orofaciodigital syndrome XIV (OFD14), a ciliopathy resulting in malformations of the oral cavity, face and digits. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
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