Alkyl DHAP synthase (AGPS) (NM_003659) Human Tagged ORF Clone

CAT#: RC220918

AGPS (Myc-DDK-tagged)-Human alkylglycerone phosphate synthase (AGPS)

Product Images

Circular map for RC220918

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  Western blot validation of overexpression lysate (Cat# LY401211) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220918 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AGPS protein (Cat# TP320918). The protein was produced from HEK293T cells transfected with AGPS cDNA clone (Cat# RC220918) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alkyl DHAP synthase
• Synonyms: ADAP-S; ADAS; ADHAPS; ADPS; ALDHPSY; RCDP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220918 representing NM_003659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCGGCGGCTGCAGCGGGTGGGACTGGCTTGGGCGCGGGCGCGAGCTACGGGTCTGCAGCGG
  ACCGGGACCGGGACCCGGACCCGGACCGCGCCGGGCGGAGGCTGCGGGTTCTCTCTGGCCATCTGCTGGG
  CCGGCCCCGGGAGGCTCTGAGTACCAATGAGTGCAAAGCGCGGAGAGCCGCGTCGGCGGCCACGGCAGCG
  CCCACGGCCACTCCCGCCGCGCAGGAGTCGGGCACCATCCCAAAGAAGCGGCAAGAAGTTATGAAATGGA
  ATGGATGGGGATATAATGATTCTAAATTCATCTTCAATAAGAAGGGCCAAATTGAATTGACTGGGAAAAG
  GTACCCTCTTAGTGGCATGGGTTTACCAACATTTAAAGAATGGATCCAAAATACCCTTGGAGTAAATGTG
  GAGCATAAAACTACCTCTAAAGCATCCTTAAATCCTAGTGATACACCTCCTTCTGTTGTAAATGAAGATT
  TTCTTCATGACCTTAAAGAAACTAATATTTCATATTCACAAGAGGCAGATGATCGAGTATTTAGAGCTCA
  TGGTCATTGTCTTCATGAGATATTTTTGCTCAGGGAAGGAATGTTTGAGCGAATTCCTGATATAGTTTTA
  TGGCCAACATGCCATGATGATGTAGTTAAGATTGTGAATCTAGCTTGCAAATATAATCTTTGTATCATAC
  CAATTGGTGGAGGAACAAGTGTTTCATATGGCCTGATGTGTCCTGCAGATGAGACAAGAACAATTATTTC
  TTTGGACACTTCACAAATGAATCGAATTCTCTGGGTTGATGAGAACAATTTGACAGCTCATGTAGAGGCT
  GGCATAACAGGACAAGAGTTGGAAAGACAGCTTAAAGAAAGTGGTTATTGTACAGGTCATGAACCAGATT
  CCCTGGAGTTCAGTACTGTAGGAGGATGGGTATCTACTCGCGCATCAGGCATGAAGAAGAATATCTATGG
  CAATATCGAGGACCTGGTGGTTCATATAAAAATGGTAACACCTAGAGGTATAATAGAAAAAAGCTGTCAA
  GGACCTCGTATGTCAACAGGCCCTGATATCCATCACTTCATCATGGGATCTGAAGGAACTCTTGGTGTAA
  TAACAGAAGCTACAATAAAAATCAGACCAGTCCCTGAATACCAAAAGTATGGCTCAGTAGCTTTCCCTAA
  TTTTGAACAAGGAGTAGCCTGTTTAAGAGAAATTGCAAAACAGAGATGTGCTCCGGCATCTATTCGCCTC
  ATGGACAACAAGCAGTTTCAGTTTGGTCATGCTCTTAAACCTCAGGTTTCCTCTATTTTTACATCATTTT
  TGGACGGATTAAAAAAGTTTTATATTACAAAGTTTAAAGGATTTGACCCAAATCAGCTAAGTGTAGCCAC
  ATTACTGTTTGAGGGGGATCGTGAGAAGGTTCTTCAACATGAAAAACAAGTGTATGATATTGCTGCAAAA
  TTTGGTGGGTTGGCAGCTGGAGAAGATAATGGACAGAGAGGTTATTTGCTGACCTATGTTATTGCATACA
  TTCGAGACTTGGCTTTGGAATACTATGTATTAGGAGAATCTTTTGAGACTTCTGCTCCTTGGGACAGGGT
  GGTAGATCTCTGTAGAAATGTAAAAGAAAGAATAACAAGGGAATGCAAAGAGAAGGGTGTTCAGTTTGCT
  CCTTTTTCTACATGCAGGGTGACGCAGACTTACGATGCAGGTGCTTGTATCTACTTCTATTTTGCCTTTA
  ACTACAGGGGAATTAGTGACCCACTGACCGTATTTGAACAAACTGAGGCAGCTGCTAGAGAAGAAATCCT
  TGCTAATGGAGGGAGCCTGTCACATCACCATGGAGTGGGCAAGTTACGGAAGCAATGGCTAAAGGAAAGT
  ATCTCTGATGTCGGCTTTGGGATGCTGAAGTCTGTCAAGGAATATGTGGACCCCAATAACATCTTTGGAA
  ACAGAAACCTTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220918 representing NM_003659
  Red=Cloning site Green=Tags(s)
  
  MAEAAAAAGGTGLGAGASYGSAADRDRDPDPDRAGRRLRVLSGHLLGRPREALSTNECKARRAASAATAA
  PTATPAAQESGTIPKKRQEVMKWNGWGYNDSKFIFNKKGQIELTGKRYPLSGMGLPTFKEWIQNTLGVNV
  EHKTTSKASLNPSDTPPSVVNEDFLHDLKETNISYSQEADDRVFRAHGHCLHEIFLLREGMFERIPDIVL
  WPTCHDDVVKIVNLACKYNLCIIPIGGGTSVSYGLMCPADETRTIISLDTSQMNRILWVDENNLTAHVEA
  GITGQELERQLKESGYCTGHEPDSLEFSTVGGWVSTRASGMKKNIYGNIEDLVVHIKMVTPRGIIEKSCQ
  GPRMSTGPDIHHFIMGSEGTLGVITEATIKIRPVPEYQKYGSVAFPNFEQGVACLREIAKQRCAPASIRL
  MDNKQFQFGHALKPQVSSIFTSFLDGLKKFYITKFKGFDPNQLSVATLLFEGDREKVLQHEKQVYDIAAK
  FGGLAAGEDNGQRGYLLTYVIAYIRDLALEYYVLGESFETSAPWDRVVDLCRNVKERITRECKEKGVQFA
  PFSTCRVTQTYDAGACIYFYFAFNYRGISDPLTVFEQTEAAAREEILANGGSLSHHHGVGKLRKQWLKES
  ISDVGFGMLKSVKEYVDPNNIFGNRNLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003659
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003659.4
• RefSeq Size: 2074 bp
• RefSeq ORF: 1977 bp
• Locus ID: 8540
• UniProt ID: O00116
• Cytogenetics: 2q31.2
• Domains: FAD-oxidase_C, FAD_binding_4
• Protein Pathways: Ether lipid metabolism, Metabolic pathways
• MW: 72.91 kDa
• Gene Summary: This gene is a member of the FAD-binding oxidoreductase/transferase type 4 family. It encodes a protein that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetonephosphate (DHAP) is converted to alkyl-DHAP by the addition of a long chain alcohol and the removal of a long-chain acid anion. The protein is localized to the inner aspect of the peroxisomal membrane and requires FAD as a cofactor. Mutations in this gene have been associated with rhizomelic chondrodysplasia punctata, type 3 and Zellweger syndrome. [provided by RefSeq, Jul 2008]