ZNF429 (NM_001001415) Human Tagged ORF Clone

CAT#: RC220861

ZNF429 (Myc-DDK-tagged)-Human zinc finger protein 429 (ZNF429)

Product Images

Circular map for RC220861

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF429
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220861 representing NM_001001415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCATTGACATTTACAGATGTGGCCATAGAATTCTCTCTGGAGGAGTGGCAGTGCCTGGACACAG
  CACAACAGAACTTATATAGAAATGTGATGTTAGAGAACTACAGAAACTTGGTCTTCCTGGGTATTGCTGT
  TTCTAAGCCAGACCTAATCACTTGTCTAGAGAAAGAAAAAGAACCCTGCAAGATGAAGCGACATGAAATG
  GTGGATGAACCCCCAGTTGTGTGTTCTCATTTTGCTGAAGACTTTTGGCCAGAGCAAGACATAAAAGATT
  CTTTCCAAAAAGTGACACTGAGGAGATATGATAAACGTGGACATGAGAACTTACAATTAAGAAAAGGCTA
  TAAAACTGTAGGTGATTGTAAGCTATACAAAGGAGGTTATAATGGACTTAACCAATGTTTGACACTTACC
  CAGAGCAAAATGTATCACTGTGATATATATGTAAAAGTCTTTTATGCATTTTCAAATGCAGATAGATACA
  AGACAAGACATACTGGAAAGAAACCTTTCCAGTGTAAAAAATGTGGCAAATCATTTTGCATGCTTTCACA
  ACTAACTCAACATAAGAAAATTCATATTAGAGAGAATACCTACAGATGTAAAGAATTTGGCAATGCCTTT
  AATCAGTCCTCAGCCCTTACTAACCATAAGAGAATTTATGTTGGTGAGAAACACTACAGATGTGAAGAAT
  GTGGCAAAGCATTTAACCACTACTCAACCCTTACTAACCATAAGAGAATTCATACTGGAGAGAAACCCTA
  CAAATGTAAAGAATGTGGCAAAGCCTTTAGCAGGTACTCAACCCTTACTACCCATAAGAGAATTCATTCT
  GGAGAGAAGCCCTACAAATGTGATGAATGTGGCAAAACCTTTAGCATATCCTCAACCTTTACTAAACATA
  AGATAATTCATACTGAAGAGAAACCCTACAAATGTAAAGAATGTGGCAAAGCCTTTAACCGGTCCTCAAC
  CCTTACTAGCCATAAGAGAATACATACTGGTGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTT
  AACTGGTCTTCAACTCTTACTAAACATAAGGTAATTCATACTGGAGAGAAGCCCTACAAATGTGAAGAAT
  GTGGCAAAGCTTTTAACCAGTCTTCAAGACTTACTCGACATAAAAAAATTCATACTGGAGAGGAACCCTA
  CAAATTTGAAAAATGTGGCAGAGTTTTTACCTGTTCCTCAACACTTACTCAAGACAAGAAAATTCATACT
  GGAGAGAAACCCTACAATTGTGAAGAATGTGGCAAAGTTTTTACCTATTCCTCTACACTTACTAGACATA
  AGAGAATTCATACTGAAGAGAAACCCTATAAATGTAACGAATGTGGCAAAGCTTTTAACCGGTCCTCACA
  CCTTACTAGCCATAGGAGAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTT
  AAGCAGTCCTCAAACCTTAACAGTCATAAAAAAATTCATAGTGGAGAGAAACCCTACAAATGTGAAGAAT
  GTGGCAAAGCTTTTATCCTGTCCTCAAGACTTACTCAACATAAGAAAATTCATACTGGAGAGAAACCTTA
  CAAATGTGAAGAATGTGGCAAAGCTTTTAACCGGTCCTCAAGACTTACTCAACATAAGAAAATTCATACT
  GGAGAGAAACCCTACAAATGTAAACAATGTGACAAAGCTTTTACCCACTCCTCAAACCTTAGTAGTCATA
  AGAAAATTCATAGTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAATCGGTCCTCAAG
  ACTTACTCAACATAAGAAAATTCATACTAGAGAGAAACCTTACAAATGTGAAGAATGTGCCAAAGCTTTT
  ACCCGGTCTTCAAGACTTACTCAACATAAGAAAATTCATAGGATGGGTGTGGTGGCTCATGCCTGTAATC
  CCAGCACTTTGGGAGGCAGAGGTGGGCGGATCACGAGGTCAGGAGATCGAGACCGTCCTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220861 representing NM_001001415
  Red=Cloning site Green=Tags(s)
  
  MGPLTFTDVAIEFSLEEWQCLDTAQQNLYRNVMLENYRNLVFLGIAVSKPDLITCLEKEKEPCKMKRHEM
  VDEPPVVCSHFAEDFWPEQDIKDSFQKVTLRRYDKRGHENLQLRKGYKTVGDCKLYKGGYNGLNQCLTLT
  QSKMYHCDIYVKVFYAFSNADRYKTRHTGKKPFQCKKCGKSFCMLSQLTQHKKIHIRENTYRCKEFGNAF
  NQSSALTNHKRIYVGEKHYRCEECGKAFNHYSTLTNHKRIHTGEKPYKCKECGKAFSRYSTLTTHKRIHS
  GEKPYKCDECGKTFSISSTFTKHKIIHTEEKPYKCKECGKAFNRSSTLTSHKRIHTGEKPYKCEECGKAF
  NWSSTLTKHKVIHTGEKPYKCEECGKAFNQSSRLTRHKKIHTGEEPYKFEKCGRVFTCSSTLTQDKKIHT
  GEKPYNCEECGKVFTYSSTLTRHKRIHTEEKPYKCNECGKAFNRSSHLTSHRRIHTGEKPYKCEECGKAF
  KQSSNLNSHKKIHSGEKPYKCEECGKAFILSSRLTQHKKIHTGEKPYKCEECGKAFNRSSRLTQHKKIHT
  GEKPYKCKQCDKAFTHSSNLSSHKKIHSGEKPYKCEECGKAFNRSSRLTQHKKIHTREKPYKCEECAKAF
  TRSSRLTQHKKIHRMGVVAHACNPSTLGGRGGRITRSGDRDRPG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001415
• ORF Size: 2022 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001415.4
• RefSeq Size: 2361 bp
• RefSeq ORF: 2025 bp
• Locus ID: 353088
• UniProt ID: Q86V71
• Cytogenetics: 19p12
• MW: 78 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]