DENND4B (NM_014856) Human Tagged ORF Clone

CAT#: RC220670

DENND4B (Myc-DDK-tagged)-Human DENN/MADD domain containing 4B (DENND4B)

Product Images

Circular map for RC220670

• 

  Western blot validation of overexpression lysate (Cat# LY414984) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220670 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DENND4B protein (Cat# TP320670). The protein was produced from HEK293T cells transfected with DENND4B cDNA clone (Cat# RC220670) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DENND4B
• Synonyms: KIAA0476
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220670 representing NM_014856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGAGCGGCCCCCCCGGCTGGTGGATTACTTCGTGGTAGCTGGGCTTGCAGGGAACGGAGCAC
  CCATCCCTGAGGAAACGTGGGTTCCTGAACCCAGTGGGCCCCTGCGCCCTCCCCGGCCAGCTGAGCCCAT
  CACAGATGTGGCAGTCATCGCTAGGGCACTGGGCGAGGAAGTGCCCCAGGGCTACACATGCATCCAGGCT
  TCTGCTGGGGGCCACCCCTTGGAACTCAGTGCTGGACTTCTGGGTGGAACTCAACCCGTCATCTGCTACC
  GCAGGGGCCGTGACAAGCCCCCCCTCGTTGAGCTGGGGGTGTTGTATGAGGGGAAGGAACGTCCCAAGCC
  TGGCTTCCAAGTGCTTGACACGACACCCTACAGCCACTCAGCAAACCTGGCCCCTCCAGGCCCCGGGCAC
  CCCCGCACCTACCTCACTTACCGGCGGGCAGCAGAGGGGGCAGGGCTGCATGCCCTGGGCATCACTGACC
  TCTGCCTGGTGCTGCCCAGTAAGGGCGAGGGCACTCCTCATACTTACTGCCGGCTGCCCCGCAACCTCAA
  CCCTGGCATGTGGGGCCCAGCAGTGTACCTGTGCTATAAGGTGGGCCTGGCGAAGGCCAACACGCTGGTG
  TACGAGGCAGAGCTGCTGGGCCGCTACCCGGAGGAGGACAATGAGGCGTTCCCGCTGCCCGAGTCAGTGC
  CCGTCTTCTGCCTGCCCATGGGGGCCACTATCGAGTGCTGGCCTGCCCAGACCAAGTACCCCGTGCCCGT
  CTTCTCCACCTTTGTGCTCACGGGTGCAGCTGGTGATAAGGTGTATGGTGCCGCCCTGCAGTTCTACGAG
  GCGTTCCCAAGGGCCAGGCTATCAGAGCGACAGGCACGGGCACTGGGCCTGCTGAGCGCCGTGGAGCGGG
  GTCGGGCACTGGGGGGCAGAGCTGTGCGCAGCCGGCGTGCCATCGCTGTGCTGTCCCGCTGGCCTGCCTT
  CCCTGCCTTCCGCGCCTTCCTCACCTTCCTTTACCGCTACTCCGTCTCAGGCCCCCACCGCCTACCCTTG
  GAAGCGCACATCTCCCACTTCATTCACAACGTTCCCTTCCCTTCCCCACAGAGACCCCGCATCCTAGTGC
  AGATGTCTCCCTATGACAACTTGCTCCTCTGTCAGCCTGTATCCTCACCCCTGCCCCTCAGTGGTGCCAG
  CTTCCTGCAGCTGCTGCAGAGCCTGGGCCCTGAGCTGGCTATCACACTGCTGCTGGCTGTGCTCACAGAG
  CACAAGCTGCTAGTCCACTCGCTGCGGCCAGACCTGCTCACCAGCGTCTGTGAGGCCCTCGTCTCGATGA
  TCTTCCCACTGCACTGGCAGTGCCCCTACATTCCTCTGTGCCCGCTGGTGCTGGCAGATGTGCTGAGTGC
  CCCAGTGCCCTTCATTGTGGGTATCCACTCCAGCTACTTTGATCTGCATGACCCGCCTGCTGATGTCATC
  TGTGTAGACCTTGATACCAACACGCTCTTCCAGACTGAGGAAAAGAAGCTCCTCTCCCCTCGGACCCTGC
  CCCGCAGACCCTACAAGGTTCTGCTGGCCACACTGACAAACCTGTACCAGCAGCTGGACCAGACATACAC
  TGGACCTGAGGAGGAAGCATCCCTGGAGTTCCTACTGACAGACTACGAGGCAGTGTGTGGCCGCAGGGCC
  CGGCTGGAGCGCGAAGTCCAAGGAGCCTTCCTCCGCTTCATGGCCTGTCTGCTCAAGGGCTACCGGGTCT
  TCCTGCGCCCACTCACCCAGGCCCCCTCCGAGGGAGCTCGTGATGTTGACAACCTTTTCTTCCTGCAGGG
  CTTCCTCAAATCCCGGGAACGCTCCAGCCACAAACTTTACTCTCAGCTGCTGCACACACAGATGTTCTCA
  CAGTTCATTGAGGAGTGCTCTTTTGGCTCTGCTCGCCATGCTGCCCTTGAATTCTTTGACTCTTGTGTTG
  AAAAGGTCCACCCAGAGCAGGAGAAGCCTGAGCCGACACCCTTAGTGGAGCTAGAGGAGCTGTCAGGAAG
  TGAGCTCACTGTCTTTATCACACCTCCCGAGGAGCCTGCCTTACCAGAGGGCAGTGAATCCACTCCCCAG
  TACTGCTATGATGGATTCCCAGAGCTACGGGCTGAGTTGTTTGAGTCTCTTCAAGAGCAACCTGGGGCCC
  TGCCTGTGCCAGGCCCTTCCCGTAGCGCCCCCAGCAGTCCTGCTCCTCGCCGTACCAAACAGGAGATGAA
  AGTTGCACAGCGGATGGCACAGAAGTCAGCAGCTGTGCCTGAGCTGTGGGCCCGGTGCCTGCTGGGGCAC
  TGCTATGGGCTGTGGTTCCTGTGTCTGCCTGCCTATGTGCGGTCGGCACCCTCCCGAGTGCAGGCACTGC
  ACACAGCCTACCATGTGCTGCGCCAGATGGAGAGCGGCAAGGTGGTGCTCCCTGATGAGGTGTGTTACCG
  GGTACTGATGCAGCTCTGCTCACACTATGGGCAGCCTGTGCTGTCTGTGCGGGTCATGCTGGAGATGCGT
  CAGGCAGGCATTGTGCCCAACACCATCACCTATGGCTACTACAATAAGGCTGTGTTGGAAAGCAAGTGGC
  CGTCTGGCACACCAGGTGGGCGTCTGCGCTGGGCCAAGCTCCGGAATGTTGTCCTGGGGGCTGCTCAGTT
  CCGCCAGCCCTTGAGAGAACGGCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAG
  GAGCAGGTGTCAGCACATCAAGAGGCAGGCAGCTCCCAGGCAGAGCCCTATTTGGAGCGCCCTTCCCCTA
  CTCGCCCTCTTCAGCGCCAGACTACTTGGGCTGGGCGAAGTCTGAGAGACCCAGCCTCACCCCCTGGACG
  CCTGGTGAAGAGTGGTAGCCTGGGCAGTGCCCGAGGGGCACAGCCCACTGTGGAGGCCGGTGTGGCCCAC
  ATGATAGAGGCCTTGGGGGTCCTGGAACCCCGGGGATCACCTGTGCCCTGGCACGATGGAAGTCTCTCAG
  ACCTGAGCCTGACAGGGGAGGAGCCGCTCCCTGGAGGCAGCCCAGGGGGCTCAGGCTCAGCCCTGAGTGC
  CCAGTCCACTGAGGCCCTGGAAGGGCTAAGTGGGCGGGGACCCAAGGCTGGTGGGCGACAGGATGAGGCA
  GGCACCCCCCGACGAGGGCTGGGTGCCCGCCTCCAACAGCTGCTCACTCCTTCCCGCCACTCCCCTGCCT
  CCCGCATTCCCCCACCTGAGCTGCCTCCTGACCTGCCACCCCCAGCCCGCCGCAGCCCCATGGACAGTCT
  TCTGCACCCCCGGGAGCGCCCTGGATCCACTGCCTCCGAGAGCTCAGCCTCTCTGGGCAGTGAGTGGGAC
  CTCTCAGAATCTTCTCTCAGCAACCTGAGTCTTCGCCGTTCCTCAGAGCGCCTCAGTGACACCCCTGGAT
  CCTTCCAGTCACCTTCCCTGGAAATTCTGCTGTCCAGCTGCTCCCTGTGCCGTGCCTGTGATTCGCTGGT
  GTATGATGAGGAAATCATGGCTGGCTGGGCACCTGATGACTCTAACCTCAACACAACCTGCCCCTTCTGC
  GCCTGCCCCTTTGTGCCCCTGCTCAGTGTCCAGACCCTTGATTCCCGGCCCAGTGTCCCCAGCCCCAAAT
  CTGCTGGTGCCAGTGGCAGCAAAGATGCTCCTGTCCCTGGTGGTCCTGGCCCTGTGCTCAGTGACCGAAG
  GCTCTGCCTTGCTCTGGATGAGCCCCAGCTCTGCAACGGGCACATGGGGGGAGCCTCCCGGCGGGTTGAG
  AGTGGGGCATGGGCATACCTGAGCCCCCTGGTGCTGCGTAAGGAGCTGGAGTCGCTGGTAGAGAACGAGG
  GCAGTGAGGTGCTGGCGTTGCCTGAACTGCCCTCTGCCCACCCCATCATCTTCTGGAACCTTTTGTGGTA
  TTTCCAACGGCTACGCCTGCCCAGTATTCTACCAGGCCTGGTGCTGGCCTCCTGTGATGGGCCTTCGCAC
  TCCCAGGCCCCATCTCCTTGGCTAACCCCTGATCCAGCCTCTGTTCAGGTACGGCTGCTGTGGGATGTAC
  TGACCCCTGACCCCAATAGCTGCCCACCTCTCTATGTGCTCTGGAGGGTCCACAGCCAGATCCCCCAGCG
  GGTGGTATGGCCAGGCCCTGTACCTGCATCCCTTAGTTTGGCACTGTTGGAGTCAGTGCTGCGCCATGTT
  GGACTCAATGAAGTGCACAAGGCTGTGGGGCTCCTGCTGGAAACTCTAGGGCCCCCACCCACTGGCCTGC
  ACCTGCAGAGGGGAATCTACCGTGAGATATTATTCCTGACAATGGCTGCTCTGGGCAAGGACCACGTGGA
  CATAGTGGCCTTCGATAAGAAGTACAAGTCTGCCTTTAACAAGCTGGCCAGCAGCATGGGCAAGGAGGAG
  CTGAGGCACCGGCGGGCGCAGATGCCCACTCCCAAGGCCATTGACTGCCGAAAATGTTTTGGAGCACCTC
  CAGAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220670 representing NM_014856
  Red=Cloning site Green=Tags(s)
  
  MAEERPPRLVDYFVVAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQA
  SAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGH
  PRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKANTLV
  YEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYE
  AFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPL
  EAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTE
  HKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVI
  CVDLDTNTLFQTEEKKLLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRA
  RLEREVQGAFLRFMACLLKGYRVFLRPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLHTQMFS
  QFIEECSFGSARHAALEFFDSCVEKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPALPEGSESTPQ
  YCYDGFPELRAELFESLQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGH
  CYGLWFLCLPAYVRSAPSRVQALHTAYHVLRQMESGKVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMR
  QAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERQQQQQQQQQQQQQQQQ
  EQVSAHQEAGSSQAEPYLERPSPTRPLQRQTTWAGRSLRDPASPPGRLVKSGSLGSARGAQPTVEAGVAH
  MIEALGVLEPRGSPVPWHDGSLSDLSLTGEEPLPGGSPGGSGSALSAQSTEALEGLSGRGPKAGGRQDEA
  GTPRRGLGARLQQLLTPSRHSPASRIPPPELPPDLPPPARRSPMDSLLHPRERPGSTASESSASLGSEWD
  LSESSLSNLSLRRSSERLSDTPGSFQSPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFC
  ACPFVPLLSVQTLDSRPSVPSPKSAGASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGGASRRVE
  SGAWAYLSPLVLRKELESLVENEGSEVLALPELPSAHPIIFWNLLWYFQRLRLPSILPGLVLASCDGPSH
  SQAPSPWLTPDPASVQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHV
  GLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLASSMGKEE
  LRHRRAQMPTPKAIDCRKCFGAPPEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014856
• ORF Size: 4488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014856.3
• RefSeq Size: 5688 bp
• RefSeq ORF: 4491 bp
• Locus ID: 9909
• UniProt ID: O75064
• Cytogenetics: 1q21.3
• Domains: DENN, PPR, dDENN
• MW: 163.7 kDa
• Gene Summary: Guanine nucleotide exchange factor (GEF) which may activate RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.[UniProtKB/Swiss-Prot Function]