Transglutaminase 3 (TGM3) (NM_003245) Human Tagged ORF Clone

CAT#: RC220515

TGM3 (Myc-DDK-tagged)-Human transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase) (TGM3)

Product Images

Circular map for RC220515

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  Western blot validation of overexpression lysate (Cat# LY418814) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220515 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TGM3 protein (Cat# TP320515). The protein was produced from HEK293T cells transfected with TGM3 cDNA clone (Cat# RC220515) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Transglutaminase 3
• Synonyms: TGE; UHS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220515 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTCTAGGAGTCCAGAGTATCAACTGGCAGAAGGCCTTCAACCGACAAGCGCATCACACAGACA
  AGTTCTCCAGCCAGGAGCTCATCTTGCGGAGAGGCCAAAACTTCCAGGTCTTAATGATCATGAACAAAGG
  CCTTGGCTCTAACGAAAGACTGGAGTTCATTGTCTCCACAGGGCCTTACCCCTCAGAGTCGGCCATGACG
  AAGGCTGTGTTTCCACTCTCCAATGGCAGTAGTGGTGGCTGGAGTGCGGTGCTTCAGGCCAGCAATGGCA
  ATACTCTGACTATCAGCATCTCCAGTCCTGCCAGCGCACCCATAGGACGGTACACAATGGCCCTCCAGAT
  CTTCTCCCAGGGCGGCATCTCCTCTGTGAAACTTGGGACGTTCATACTGCTTTTTAACCCCTGGCTGAAT
  GTGGATAGCGTCTTTATGGGTAACCACGCTGAGAGAGAAGAGTATGTTCAGGAAGATGCCGGCATCATCT
  TTGTGGGAAGCACAAACCGAATTGGCATGATTGGCTGGAACTTTGGACAGTTTGAAGAAGACATTCTCAG
  CATCTGCCTCTCAATCTTGGATAGGAGTCTGAATTTCCGCCGTGACGCTGCTACTGATGTGGCCAGCAGA
  AATGACCCCAAATACGTTGGCCGGGTGCTGAGTGCCATGATCAATAGCAATGATGACAATGGTGTGCTTG
  CTGGGAATTGGAGCGGCACTTACACCGGTGGCCGGGACCCAAGGAGCTGGAACGGCAGCGTGGAGATCCT
  CAAAAATTGGAAAAAATCTGGCTTCAGCCCAGTCCGATATGGCCAGTGCTGGGTCTTTGCTGGGACCCTC
  AACACAGCGCTGCGGTCTTTGGGGATTCCTTCCCGGGTGATCACCAACTTCAACTCAGCTCATGACACAG
  ACCGAAATCTCAGTGTGGATGTGTACTACGACCCCATGGGAAACCCCCTGGACAAGGGTAGTGATAGCGT
  ATGGAATTTCCATGTCTGGAATGAAGGCTGGTTTGTGAGGTCTGACCTGGGCCCCTCGTACGGTGGATGG
  CAGGTGTTGGATGCTACCCCGCAGGAAAGAAGCCAAGGGGTGTTCCAGTGCGGCCCCGCTTCGGTCATTG
  GTGTTCGAGAGGGTGATGTGCAGCTGAACTTCGACATGCCCTTTATCTTCGCGGAGGTTAATGCCGACCG
  CATCACCTGGCTGTACGACAACACCACTGGCAAACAGTGGAAGAATTCCGTGAACAGTCACACCATTGGC
  AGGTACATCAGCACCAAGGCGGTGGGCAGCAATGCTCGCATGGACGTCACGGACAAGTACAAGTACCCAG
  AAGGCTCTGACCAGGAAAGACAAGTGTTCCAAAAGGCTTTGGGGAAACTTAAACCCAACACGCCATTTGC
  CGCGACGTCTTCAATGGGTTTGGAAACAGAGGAACAGGAGCCCAGCATCATCGGGAAGCTGAAGGTCGCT
  GGCATGCTGGCAGTAGGCAAAGAAGTCAACCTGGTCCTACTGCTCAAAAACCTGAGCAGGGATACGAAGA
  CAGTGACAGTGAACATGACAGCCTGGACCATCATCTACAACGGCACGCTTGTACATGAAGTGTGGAAGGA
  CTCTGCCACAATGTCCCTGGACCCTGAGGAAGAGGCAGAACATCCCATAAAGATCTCGTACGCTCAGTAT
  GAGAAGTACCTGAAGTCAGACAACATGATCCGGATCACAGCGGTGTGCAAGGTCCCAGATGAGTCTGAGG
  TGGTGGTGGAGCGGGACATCATCCTGGACAACCCCACCTTGACCCTGGAGGTGCTGAACGAGGCTCGTGT
  GCGGAAGCCTGTGAACGTGCAGATGCTCTTCTCCAATCCACTGGATGAGCCGGTGAGGGACTGCGTGCTG
  ATGGTGGAGGGAAGCGGCCTGCTGTTGGGTAACCTGAAGATCGACGTGCCGACCCTAGGGCCCAAGGAGC
  GGTCCCGGGTCCGTTTTGATATCCTGCCCTCCCGGAGTGGCACCAAGCAACTGCTCGCCGACTTCTCCTG
  CAACAAGTTCCCTGCAATCAAGGCCATGTTGTCCATCGATGTAGCCGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220515 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALGVQSINWQKAFNRQAHHTDKFSSQELILRRGQNFQVLMIMNKGLGSNERLEFIVSTGPYPSESAMT
  KAVFPLSNGSSGGWSAVLQASNGNTLTISISSPASAPIGRYTMALQIFSQGGISSVKLGTFILLFNPWLN
  VDSVFMGNHAEREEYVQEDAGIIFVGSTNRIGMIGWNFGQFEEDILSICLSILDRSLNFRRDAATDVASR
  NDPKYVGRVLSAMINSNDDNGVLAGNWSGTYTGGRDPRSWNGSVEILKNWKKSGFSPVRYGQCWVFAGTL
  NTALRSLGIPSRVITNFNSAHDTDRNLSVDVYYDPMGNPLDKGSDSVWNFHVWNEGWFVRSDLGPSYGGW
  QVLDATPQERSQGVFQCGPASVIGVREGDVQLNFDMPFIFAEVNADRITWLYDNTTGKQWKNSVNSHTIG
  RYISTKAVGSNARMDVTDKYKYPEGSDQERQVFQKALGKLKPNTPFAATSSMGLETEEQEPSIIGKLKVA
  GMLAVGKEVNLVLLLKNLSRDTKTVTVNMTAWTIIYNGTLVHEVWKDSATMSLDPEEEAEHPIKISYAQY
  EKYLKSDNMIRITAVCKVPDESEVVVERDIILDNPTLTLEVLNEARVRKPVNVQMLFSNPLDEPVRDCVL
  MVEGSGLLLGNLKIDVPTLGPKERSRVRFDILPSRSGTKQLLADFSCNKFPAIKAMLSIDVAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003245
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003245.4
• RefSeq Size: 2679 bp
• RefSeq ORF: 2082 bp
• Locus ID: 7053
• UniProt ID: Q08188
• Cytogenetics: 20p13
• Protein Families: Druggable Genome
• MW: 76.8 kDa
• Gene Summary: Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008]