Collagen XVII (COL17A1) (NM_000494) Human Tagged ORF Clone

CAT#: RC220502

COL17A1 (Myc-DDK-tagged)-Human collagen, type XVII, alpha 1 (COL17A1)

Product Images

Circular map for RC220502

• 

  Western blot validation of overexpression lysate (Cat# LY424682) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220502 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Collagen XVII
• Synonyms: BA16H23.2; BP180; BPA-2; BPAG2; EBR2A; ERED; JEB-I; LAD-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220502 representing NM_000494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTAACCAAGAAAAACAAACGAGATGGAACTGAAGTCACTGAGAGAATTGTCACTGAAACAGTAA
  CCACAAGACTTACATCCTTACCACCAAAAGGCGGGACCAGCAATGGCTATGCTAAAACAGCCTCTCTTGG
  TGGAGGGAGCCGGCTGGAGAAACAAAGCCTGACTCATGGCAGCAGCGGCTACATAAACTCAACTGGAAGC
  ACACGAGGCCATGCCTCCACCTCTAGTTACAGGAGGGCTCACTCACCTGCCTCCACTCTGCCCAACTCCC
  CAGGCTCAACCTTTGAAAGGAAAACTCACGTTACCCGCCATGCGTATGAAGGGAGCTCCAGTGGCAACTC
  TTCTCCGGAGTACCCTCGGAAGGAATTTGCATCTTCTTCAACCAGAGGACGGAGTCAAACACGAGAGAGT
  GAAATTCGAGTTCGACTGCAGAGTGCGTCCCCATCCACCCGATGGACAGAATTGGATGATGTTAAGCGTT
  TGCTCAAGGGGAGTCGATCGGCAAGTGTGAGCCCCACCCGGAATTCCTCCAACACACTCCCCATCCCCAA
  GAAAGGCACTGTGGAGACCAAAATTGTGACAGCGAGCTCCCAGTCGGTGTCAGGCACCTACGATGCAATG
  ATCCTGGATGCCAACCTTCCCTCCCATGTGTGGTCCTCCACCCTGCCCGCGGGGTCCTCCATGGGGACCT
  ATCACAACAACATGACAACCCAGAGCTCATCCCTCCTCAACACCAATGCCTACTCTGCGGGATCAGTCTT
  CGGAGTTCCAAACAACATGGCGTCCTGCTCACCCACTTTGCACCCTGGACTCAGCACATCCTCCTCAGTG
  TTTGGCATGCAGAACAATCTGGCCCCCAGCTTGACCACCCTGTCCCATGGCACCACCACCACTTCCACAG
  CATATGGGGTGAAGAAAAACATGCCCCAGAGTCCTGCGGCTGTGAACACTGGCGTTTCCACCTCCGCCGC
  CTGCACCACAAGTGTGCAGAGCGATGACCTTTTGCACAAGGACTGCAAGTTCCTGATCCTAGAGAAAGAC
  AACACACCTGCTAAGAAGGAGATGGAGCTGCTCATCATGACCAAGGACAGCGGGAAGGTCTTTACAGCCT
  CCCCTGCCAGCATCGCTGCAACTTCTTTTTCAGAAGACACCCTAAAAAAAGAAAAGCAAGCTGCCTACAA
  TGCTGACTCAGGCCTAAAAGCCGAAGCTAATGGAGACCTGAAGACTGTGTCCACAAAGGGCAAGACCACC
  ACTGCAGATATCCACAGCTACAGCAGCAGTGGTGGTGGTGGCAGTGGAGGAGGTGGCGGTGTTGGTGGCG
  CTGGCGGCGGCCCTTGGGGACCAGCGCCAGCCTGGTGCCCCTGCGGCTCCTGCTGCAGCTGGTGGAAGTG
  GCTGCTGGGCCTGCTGCTCACCTGGCTGCTACTCCTGGGGCTGCTCTTCGGCCTCATTGCTCTGGCGGAG
  GAGGTGAGGAAGCTGAAGGCGCGTGTGGATGAGCTGGAGAGGATCAGGAGGAGCATACTGCCCTATGGGG
  ACAGCATGGATAGAATAGAAAAGGACCGCCTCCAGGGCATGGCACCCGCGGCGGGAGCAGACCTGGACAA
  AATTGGGCTGCACAGTGACAGCCAGGAGGAGCTCTGGATGTTCGTGAGGAAGAAGCTAATGATGGAACAG
  GAAAATGGAAATCTCCGAGGAAGCCCTGGCCCTAAAGGTGACATGGGAAGTCCAGGCCCTAAAGGAGATC
  GAGGGTTCCCTGGGACTCCAGGTATCCCTGGGCCCTTGGGCCACCCAGGTCCACAAGGACCAAAGGGTCA
  AAAAGGCAGCGTGGGAGATCCTGGCATGGAAGGCCCCATGGGCCAGAGAGGGCGAGAAGGCCCCATGGGA
  CCTCGTGGTGAGGCAGGGCCTCCTGGATCTGGAGAGAAAGGGGAAAGAGGGGCTGCTGGTGAACCAGGTC
  CTCATGGCCCACCTGGTGTCCCAGGTTCTGTGGGTCCCAAAGGTTCCAGCGGCTCTCCTGGCCCACAGGG
  CCCTCCAGGTCCTGTAGGTCTCCAAGGGCTCCGAGGTGAAGTAGGACTTCCTGGTGTCAAAGGTGACAAA
  GGACCAGTGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAAGGACCTCGAGGCCTCACAGGCGAGC
  CTGGCATGAGAGGTTTGCCTGGTGCTGTTGGTGAGCCCGGGGCTAAAGGAGCAATGGGTCCTGCTGGCCC
  AGACGGACACCAAGGCCCAAGAGGTGAACAAGGTCTTACTGGGATGCCTGGAATCCGTGGCCCACCAGGA
  CCTTCTGGAGACCCAGGAAAGCCAGGTCTCACAGGACCCCAGGGACCTCAGGGACTTCCCGGTACCCCTG
  GCCGACCAGGAATAAAAGGTGAACCAGGAGCTCCAGGCAAGATCGTGACTTCGGAGGGGTCATCGATGCT
  CACTGTCCCAGGCCCCCCAGGACCTCCTGGAGCCATGGGACCCCCAGGACCTCCAGGTGCCCCAGGCCCT
  GCCGGCCCAGCTGGTCTCCCAGGACATCAAGAAGTTCTTAATTTACAAGGTCCCCCAGGCCCACCCGGCC
  CACGCGGGCCACCAGGGCCTTCCATTCCAGGCCCACCAGGACCCCGAGGCCCACCAGGGGAGGGTTTGCC
  AGGCCCACCAGGCCCACCAGGATCGTTCCTGTCCAACTCAGAAACCTTCCTCTCCGGCCCCCCAGGCCCA
  CCTGGCCCCCCAGGTCCCAAGGGAGACCAAGGTCCCCCAGGCCCCAGAGGACACCAAGGCGAGCAAGGCC
  TCCCAGGTTTCTCAACCTCAGGGTCCAGTTCTTTCGGACTCAACCTTCAGGGACCACCAGGCCCACCTGG
  CCCCCAGGGACCAAAAGGTGACAAAGGTGATCCGGGTGTTCCAGGGGCTCTTGGCATTCCTAGTGGTCCT
  TCTGAAGGGGGATCATCAAGTACCATGTACGTGTCAGGCCCGCCAGGGCCCCCTGGGCCCCCTGGGCCTC
  CGGGCTCTATCAGCAGCTCTGGCCAGGAGATTCAGCAGTACATCTCTGAGTACATGCAGAGTGACAGTAT
  TAGATCTTACCTATCCGGAGTTCAGGGTCCCCCAGGCCCACCTGGTCCCCCAGGACCTGTCACCACCATC
  ACAGGCGAGACTTTCGACTACTCAGAGCTGGCAAGCCACGTTGTGAGCTACTTACGGACTTCGGGGTACG
  GTGTCAGCTTGTTCTCGTCCTCCATCTCTTCTGAAGACATCCTGGCTGTGCTGCAGCGGGATGACGTGCG
  TCAGTACCTACGTCAGTACTTGATGGGCCCTCGGGGTCCGCCAGGGCCACCAGGAGCCAGTGGAGATGGG
  TCCCTCCTGTCTTTGGACTATGCAGAGCTGAGTAGTCGCATTCTCAGCTACATGTCGAGTTCTGGGATCA
  GCATTGGGCTTCCTGGTCCCCCGGGGCCCCCTGGCTTGCCGGGAACCTCCTATGAGGAGCTCCTCTCCTT
  GCTGCGAGGGTCTGAATTCAGAGGCATCGTTGGACCCCCAGGTCCCCCGGGTCCACCAGGGATCCCAGGC
  AATGTGTGGTCCAGCATCAGCGTGGAGGACCTCTCGTCTTACTTACATACTGCCGGCTTGTCATTCATCC
  CAGGCCCTCCAGGACCTCCTGGTCCCCCAGGGCCTCGAGGGCCCCCGGGTGTCTCAGGAGCCCTGGCAAC
  CTATGCAGCTGAAAACAGCGACAGCTTCCGGAGCGAGCTGATCAGCTACCTCACAAGTCCTGATGTGCGC
  AGCTTCATTGTTGGCCCCCCAGGCCCTCCTGGGCCGCAGGGACCCCCTGGGGACAGCCGCCTCCTGTCCA
  CGGATGCCTCCCACAGTCGGGGTAGCAGCTCCTCCTCACACAGCTCATCTGTCAGGCGGGGCAGCTCCTA
  CAGCTCTTCCATGAGCACAGGAGGAGGTGGTGCAGGCTCCCTGGGTGCAGGCGGTGCCTTTGGTGAAGCT
  GCAGGAGACAGGGGTCCCTATGGCACTGACATCGGCCCAGGCGGAGGCTATGGGGCAGCAGCAGAAGGCG
  GCATGTATGCTGGCAATGGCGGACTATTGGGAGCTGACTTTGCTGGAGATCTGGATTACAATGAGCTGGC
  TGTGAGGGTGTCAGAGAGCATGCAGCGTCAGGGCCTACTGCAAGGGATGGCCTACACTGTCCAGGGCCCA
  CCAGGCCAGCCTGGGCCACAGGGGCCACCCGGCATCAGCAAGGTCTTCTCTGCCTACAGCAACGTGACTG
  CGGACCTCATGGACTTCTTCCAAACTTATGGAGCCATTCAAGGACCCCCTGGGCAAAAAGGAGAGATGGG
  CACTCCAGGACCCAAAGGTGACAGGGGCCCTGCTGGGCCACCAGGTCATCCTGGGCCACCTGGCCCTCGA
  GGACACAAGGGAGAAAAAGGAGACAAAGGTGACCAAGTCTATGCTGGGCGGAGAAGGAGAAGAAGTATTG
  CTGTCAAGCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220502 representing NM_000494
  Red=Cloning site Green=Tags(s)
  
  MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGS
  TRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRES
  EIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASSQSVSGTYDAM
  ILDANLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTNAYSAGSVFGVPNNMASCSPTLHPGLSTSSSV
  FGMQNNLAPSLTTLSHGTTTTSTAYGVKKNMPQSPAAVNTGVSTSAACTTSVQSDDLLHKDCKFLILEKD
  NTPAKKEMELLIMTKDSGKVFTASPASIAATSFSEDTLKKEKQAAYNADSGLKAEANGDLKTVSTKGKTT
  TADIHSYSSSGGGGSGGGGGVGGAGGGPWGPAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAE
  EVRKLKARVDELERIRRSILPYGDSMDRIEKDRLQGMAPAAGADLDKIGLHSDSQEELWMFVRKKLMMEQ
  ENGNLRGSPGPKGDMGSPGPKGDRGFPGTPGIPGPLGHPGPQGPKGQKGSVGDPGMEGPMGQRGREGPMG
  PRGEAGPPGSGEKGERGAAGEPGPHGPPGVPGSVGPKGSSGSPGPQGPPGPVGLQGLRGEVGLPGVKGDK
  GPVGPPGPKGDQGEKGPRGLTGEPGMRGLPGAVGEPGAKGAMGPAGPDGHQGPRGEQGLTGMPGIRGPPG
  PSGDPGKPGLTGPQGPQGLPGTPGRPGIKGEPGAPGKIVTSEGSSMLTVPGPPGPPGAMGPPGPPGAPGP
  AGPAGLPGHQEVLNLQGPPGPPGPRGPPGPSIPGPPGPRGPPGEGLPGPPGPPGSFLSNSETFLSGPPGP
  PGPPGPKGDQGPPGPRGHQGEQGLPGFSTSGSSSFGLNLQGPPGPPGPQGPKGDKGDPGVPGALGIPSGP
  SEGGSSSTMYVSGPPGPPGPPGPPGSISSSGQEIQQYISEYMQSDSIRSYLSGVQGPPGPPGPPGPVTTI
  TGETFDYSELASHVVSYLRTSGYGVSLFSSSISSEDILAVLQRDDVRQYLRQYLMGPRGPPGPPGASGDG
  SLLSLDYAELSSRILSYMSSSGISIGLPGPPGPPGLPGTSYEELLSLLRGSEFRGIVGPPGPPGPPGIPG
  NVWSSISVEDLSSYLHTAGLSFIPGPPGPPGPPGPRGPPGVSGALATYAAENSDSFRSELISYLTSPDVR
  SFIVGPPGPPGPQGPPGDSRLLSTDASHSRGSSSSSHSSSVRRGSSYSSSMSTGGGGAGSLGAGGAFGEA
  AGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGP
  PGQPGPQGPPGISKVFSAYSNVTADLMDFFQTYGAIQGPPGQKGEMGTPGPKGDRGPAGPPGHPGPPGPR
  GHKGEKGDKGDQVYAGRRRRRSIAVKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000494
• ORF Size: 4491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000494.4
• RefSeq Size: 5610 bp
• RefSeq ORF: 4494 bp
• Locus ID: 1308
• UniProt ID: Q9UMD9
• Cytogenetics: 10q25.1
• Protein Families: Transmembrane
• MW: 150.2 kDa
• Gene Summary: This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]