MSLNL (NM_001025190) Human Tagged ORF Clone

CAT#: RC220422

MSLNL (Myc-DDK-tagged)-Human mesothelin-like (MSLNL). Note: ORF is codon optimized

Product Images

Circular map for RC220422

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MSLNL
• Synonyms: C16orf37; MPFL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220422 ORF sequence, codon optimized.
  Due to the complexity of NM_001025190, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGTCTGGGGACCCAGGTATTCCTGCCCTCCACAGACTGTGCACCAGAGCCCTGTCTGCTTCCC
  CGGGACCGACCGCCCTCGGCCCTAGTACTTTCCTCCCATGGGACGTTTCCGCGAGAATGACCAGCGCCCG
  CCGCCGAACTGACGTGGGCCCACGGGTGGCTTTTACATACCCTTGCACCCAGCTGTCTCCCACCAGAGCA
  CATCGGCATCTGCCCGTTCATACTGTGACCTACCCGTGCACACAGCTCTCCCCTACCAGGGCTCACAGCT
  GCCACCTGCCGATGCACACAGTTACTTATCCATGTACTCCCTCACCAACCCGCGCCCACTCATGTCATCT
  CCCAGTTCATACTGTGACTTATACATGTACCCTGTCACCAACCCATGCATGCCTGCATGCTTCTACAGTG
  TCATGCCTGTGCTGCATTCCGGTGCATACCGTTACCTACCCATGCACGCTGAGCCCAACATGCGCGCATT
  GTCATCTGCACATGAGAGCCCGGACCCACCCCCAGCCACACGCATGCGCCGTCTATTTGTGCATCCTGTC
  TCCGACTCGGGCTCATTGCCACCTTCACGTACATACAGTTACGTACATGTGCGTCCCCGCATGCACACAT
  TCTATCACCCCCGTACATGCACCTCCAGATACCAGTTGCGCATGGGCTCAAGCCGTGCATAGGTGGCCCG
  TCCTCTGGGGGAGCAGGAGTTCATCCATGAGCAGTGGGTTGAAACAGACCCCCATGGTCGGCGTGACCGG
  TCCTCCTTGGGACCCCGACGCCCCCGCCCCCGCCCCCGCAGGGACCCTGACCGCTCACCTCATACCAGGC
  GCTCTGCAGTCATCAGGACTCACCCTGCTGCTGTCTCTGGCGGCCCACTGCTCAGGGCCCCAGGCTAAAG
  TCCTGTCTCCAGGTGGACTCGACGCTAGCGGGGCTAACCTGTGGGCCTCAGCGAATTGCAGTCTTCTGCA
  GGGATTCTGGTGCCAGCCCGCATCTCAGCTGCCCAGGGATCAGCTCTCTGCCCTTATCCAACGACTTGCC
  CTCTTGCAGGTGCCCTTGCAGGCCTGGCAGGTGTCAGGAAAGGAGTGCCGGGGTGCCGGAACGGGCGGCC
  GGGGCGATGACCCTACTTGTGGCAAAAGAGGAAGAGAGGTGGGAGGAGACTGGGGACTGCGACCACCACA
  GAGAGGTCGCGCGCCGACCGGCTGCCACGGAGAGAGTCGCGTTACTCGCGAAAGGAGATCCGCGCAACTC
  TCTTGTCTCGCAAATTTGGCCTCAAGATGCGGGCTGCAAGACGATTTTACCCTGCATCCTCCAAACTTGC
  TGTTGTTCTACAACCTGAGCCAGGTTCGCGAAGCAGACTGTCGCGCTTTTATCCGCAGGGCAGCCCAGGG
  CGACGTAGAGCTTCTGTCTCACCTTCCTGATCAAAGGGTCGCACTGTGGCGCGCTGCCGTCGCATGCCTT
  GTGGGCGCTGGCTGGGGCTGGGCTCGCTGCATTGGGGGCTCCCTCCCACTGGGGGCGGACCTGCTCGCCC
  TGCAGGGAGTGGCTCGGCTGCGCCTGTCCGCAAGCGATCAGCAGCTGCTCGGAGCGCTGGTGTGCGATAT
  GGACGCCAGCAGTATCGGCGCAGCAGACCCGCACATGCTTGAGAACCTCCGACGCTGCCCTCGGCTCACA
  GCAGCTCAGAGGATTGCCCTCAACTCTCTCCTGGCGGGTGGGAAGACCTCCCTGGGCCCTCCCGGTTCCT
  GGACCTTGGAAGGCCTCCAAGCGCTGGGCCCTCTGGCTACCTACATCAGTCCCCATCTGTGGGCTCAAGT
  GCAGGAAGCTGTTGGCTTGGGATTTTTTAGAAGCGTCGTTGCAAGCTGCCAGGTGGGGAGGCTGGGCCAG
  AGGGAGGCACGGTGCTTCGTGACCAGTTTCCTGGAATCAAAGACGAAACCCGTGTCCTCACGCCCTCGCC
  TTTCCACTGGGAACATAACCGCCGCGACACTGCGGGACGACCTTTTTCTCGTGCACTATGATTGTGCTGA
  GCTGGAATCTTGCCTGGATGGATGTATTTTGCGCACTAACCTCGATACGCTCCTGCAGCACCTCCTCCCG
  ACCGAATGCCAGCACGTGGTTAAGGCTAAGTTGGCCCAGATCTACCCCCAAGGCTTGCCCGAGGACCAGC
  TGAGACTGATCACATCTCTGGTATATCTTTACAGCCGAACGGAAATAGGCCAGTGGTCCATCACTAGTCA
  GGACACTGTGATGGCTCTGCTGGCATCTGACGTGGCTCTCGAGAACCAGACAGAAGCCGTCCTCCAGAAA
  TTCTTGGAGCATAATGGGACAGTGTCTGGCGCACTGCTCCTTGCCATTGGAGGGACAAGACTTTGCTGGA
  TGTCCCCGCACCAAATTCAGACGATTCACCCACAGGAGCTCAGACTGGCTGGAGCTCTGGATCTTAGTTC
  ATGTCCACAGAGCCGAAAAGACGTGCTGTATACCAAGGCACACGAGACTTTCGGGAGTTCCGGGACTCTG
  GCAGCTTATTACCGCTTGATGCGGCCATACCTGGGCGGGTCCCCGGGAGGGGCACAGCCACCAAGCCCAG
  TGCCCCCGGGTGGCGCCCCCGTTGAAGAGCTGCGACATCTTGCACACGCAAACATTTCAATGGACATTGA
  TACTTTCACCAGTCTGAACCCTCTCGAACTGCAGAGCCTTGACGTAGGCAATGTCACCGCCCTCCTGGGG
  CACAACGTAGGTGACCTTCAGAAAGCCAGGTCCCATCCTACCGTTAGGGCCTGGCTGAGATCCCTGAACA
  GTTCTACTCTGGGGCAGCTCGGTTTGGACGCCAGCCCAACTAGCCCGACTGGCCCCGCACACGGTACCCG
  GGGCCCCCCATCTACCACCCACCAGGTTCTCCACTTGGTACACACTTCCGGCCTGCCCACTAACGACGCG
  CAGGCTTCAACTTCCGGCTCCCTGTGGGCTCCTCTGGGCTACCTGCCCCTGGCTATGGCGCTTCCTTGTT
  CCCTTCTGTGCCTGCTCCACTGGGGGACCTGCATTCTGGTGAGCGTGGATTCTGTTGCCTCTGGCTGGTT
  GGGATCCCAGGGCAGCGGCGCCGGGAAGACGGAGGTGCTCGACTCCGCCGGCAGACCACTGGGGCTGACC
  GGACAGTTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220422 representing NM_001025190
  Red=Cloning site Green=Tags(s)
  
  MEVSGDPGIPALHRLCTRALSASPGPTALGPSTFLPWDVSARMTSARRRTDVGPRVAFTYPCTQLSPTRA
  HRHLPVHTVTYPCTQLSPTRAHSCHLPMHTVTYPCTPSPTRAHSCHLPVHTVTYTCTLSPTHACLHASTV
  SCLCCIPVHTVTYPCTLSPTCAHCHLHMRARTHPQPHACAVYLCILSPTRAHCHLHVHTVTYMCVPACTH
  SITPVHAPPDTSCAWAQAVHRWPVLWGSRSSSMSSGLKQTPMVGVTGPPWDPDAPAPAPAGTLTAHLIPG
  ALQSSGLTLLLSLAAHCSGPQAKVLSPGGLDASGANLWASANCSLLQGFWCQPASQLPRDQLSALIQRLA
  LLQVPLQAWQVSGKECRGAGTGGRGDDPTCGKRGREVGGDWGLRPPQRGRAPTGCHGESRVTRERRSAQL
  SCLANLASRCGLQDDFTLHPPNLLLFYNLSQVREADCRAFIRRAAQGDVELLSHLPDQRVALWRAAVACL
  VGAGWGWARCIGGSLPLGADLLALQGVARLRLSASDQQLLGALVCDMDASSIGAADPHMLENLRRCPRLT
  AAQRIALNSLLAGGKTSLGPPGSWTLEGLQALGPLATYISPHLWAQVQEAVGLGFFRSVVASCQVGRLGQ
  REARCFVTSFLESKTKPVSSRPRLSTGNITAATLRDDLFLVHYDCAELESCLDGCILRTNLDTLLQHLLP
  TECQHVVKAKLAQIYPQGLPEDQLRLITSLVYLYSRTEIGQWSITSQDTVMALLASDVALENQTEAVLQK
  FLEHNGTVSGALLLAIGGTRLCWMSPHQIQTIHPQELRLAGALDLSSCPQSRKDVLYTKAHETFGSSGTL
  AAYYRLMRPYLGGSPGGAQPPSPVPPGGAPVEELRHLAHANISMDIDTFTSLNPLELQSLDVGNVTALLG
  HNVGDLQKARSHPTVRAWLRSLNSSTLGQLGLDASPTSPTGPAHGTRGPPSTTHQVLHLVHTSGLPTNDA
  QASTSGSLWAPLGYLPLAMALPCSLLCLLHWGTCILVSVDSVASGWLGSQGSGAGKTEVLDSAGRPLGLT
  GQL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025190
• ORF Size: 3159 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025190.1, NP_001020361.1
• RefSeq Size: 3162 bp
• RefSeq ORF: 3161 bp
• Locus ID: 401827
• Cytogenetics: 16p13.3
• MW: 112.2 kDa