BTNL9 (NM_152547) Human Tagged ORF Clone

CAT#: RC220296

BTNL9 (Myc-DDK-tagged)-Human butyrophilin-like 9 (BTNL9)

Product Images

Circular map for RC220296

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  Western blot validation of overexpression lysate (Cat# LY407458) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220296 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BTNL9 protein (Cat# TP320296). The protein was produced from HEK293T cells transfected with BTNL9 cDNA clone (Cat# RC220296) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BTNL9
• Synonyms: BTN3; BTN8; VDLS1900
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220296 representing NM_152547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACCTCTCAGTCTCCCCAGACTCCTTGAAGCCAGTATCGCTGACCAGCAGTCTTGTCTTCCTCA
  TGCACCTCCTCCTCCTTCAGCCTGGGGAGCCGAGCTCAGAGGTCAAGGTGCTAGGCCCTGAGTATCCCAT
  CCTGGCCCTCGTCGGGGAGGAGGTGGAGTTCCCGTGCCACCTATGGCCACAGCTGGATGCCCAGCAAATG
  GAGATCCGCTGGTTCCGGAGTCAGACCTTCAATGTGGTACACCTGTACCAGGAGCAGCAGGAGCTCCCTG
  GCAGGCAGATGCCGGCGTTCCGGAACAGGACCAAGTTGGTCAAGGACGACATCGCCTATGGCAGCGTGGT
  CCTGCAGCTTCACAGCATCATCCCCTCTGACAAGGGCACATATGGCTGCCGCTTCCACTCCGACAACTTC
  TCTGGCGAAGCTCTCTGGGAACTGGAGGTAGCAGGGCTGGGCTCAGACCCTCACCTCTCCCTTGAGGGCT
  TCAAGGAAGGAGGCATTCAGCTGAGGCTCAGATCCAGTGGCTGGTACCCCAAGCCTAAGGTTCAGTGGAG
  AGACCACCAGGGACAGTGCCTGCCTCCAGAGTTTGAAGCCATCGTCTGGGATGCCCAGGACCTGTTCAGT
  CTGGAAACATCTGTGGTTGTCCGAGCGGGAGCCCTCAGCAATGTGTCCGTCTCCATCCAGAATCTCCTCT
  TGAGCCAGAAGAAAGAGTTGGTGGTCCAGATAGCAGACGTGTTCGTACCCGGAGCCTCTGCGTGGAAGAG
  CGCGTTCGTCGCGACCCTGCCGCTGCTGTTGGTCCTCGCGGCGCTGGCGCTGGGCGTCCTCCGGAAGCAG
  CGGAGAAGCCGAGAAAAGCTGAGGAAGCAGGCGGAGAAGAGACAAGAGAAACTCACTGCAGAGCTGGAAA
  AGCTTCAGACAGAGCTTGACTGGAGACGGGCTGAAGGCCAGGCTGAGTGGAGAGCAGCCCAAAAATATGC
  AGTGGATGTGACGCTGGACCCGGCCTCGGCGCACCCCAGCCTGGAGGTGTCGGAGGATGGCAAGAGCGTG
  TCTTCCCGCGGGGCGCCGCCAGGCCCGGCGCCTGGCCACCCGCAGCGGTTCTCGGAGCAGACGTGCGCGC
  TGAGCCTGGAGCGGTTCTCCGCCGGCCGCCACTACTGGGAGGTGCACGTGGGCCGCCGCAGCCGCTGGTT
  CCTGGGCGCCTGCCTGGCCGCGGTGCCGCGCGCGGGGCCTGCGCGCCTGAGCCCTGCGGCCGGCTACTGG
  GTGCTGGGGCTGTGGAACGGCTGCGAGTACTTCGTCCTGGCCCCGCACCGCGTCGCGCTCACCCTGCGCG
  TGCCCCCGCGGCGCCTGGGCGTCTTCCTGGACTACGAGGCCGGAGAGCTGTCCTTCTTCAACGTGTCCGA
  CGGCTCCCACATCTTCACCTTCCACGACACCTTCTCGGGCGCGCTCTGTGCGTACTTCAGGCCCAGGGCC
  CACGACGGCGGCGAACATCCGGATCCCCTGACCATCTGCCCGCTGCCGGTTAGAGGGACGGGCGTCCCCG
  AAGAGAACGACAGTGACACCTGGCTACAGCCCTATGAGCCCGCGGACCCCGCCCTGGACTGGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220296 representing NM_152547
  Red=Cloning site Green=Tags(s)
  
  MVDLSVSPDSLKPVSLTSSLVFLMHLLLLQPGEPSSEVKVLGPEYPILALVGEEVEFPCHLWPQLDAQQM
  EIRWFRSQTFNVVHLYQEQQELPGRQMPAFRNRTKLVKDDIAYGSVVLQLHSIIPSDKGTYGCRFHSDNF
  SGEALWELEVAGLGSDPHLSLEGFKEGGIQLRLRSSGWYPKPKVQWRDHQGQCLPPEFEAIVWDAQDLFS
  LETSVVVRAGALSNVSVSIQNLLLSQKKELVVQIADVFVPGASAWKSAFVATLPLLLVLAALALGVLRKQ
  RRSREKLRKQAEKRQEKLTAELEKLQTELDWRRAEGQAEWRAAQKYAVDVTLDPASAHPSLEVSEDGKSV
  SSRGAPPGPAPGHPQRFSEQTCALSLERFSAGRHYWEVHVGRRSRWFLGACLAAVPRAGPARLSPAAGYW
  VLGLWNGCEYFVLAPHRVALTLRVPPRRLGVFLDYEAGELSFFNVSDGSHIFTFHDTFSGALCAYFRPRA
  HDGGEHPDPLTICPLPVRGTGVPEENDSDTWLQPYEPADPALDWW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152547
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152547.5
• RefSeq Size: 3479 bp
• RefSeq ORF: 1608 bp
• Locus ID: 153579
• UniProt ID: Q6UXG8
• Cytogenetics: 5q35.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 59.5 kDa