ITIH3 (NM_002217) Human Tagged ORF Clone

CAT#: RC220252

ITIH3 (Myc-DDK-tagged)-Human inter-alpha (globulin) inhibitor H3 (ITIH3)

Product Images

Circular map for RC220252

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  Western blot validation of overexpression lysate (Cat# LY419449) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220252 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ITIH3 protein (Cat# TP320252). The protein was produced from HEK293T cells transfected with ITIH3 cDNA clone (Cat# RC220252) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITIH3
• Synonyms: H3P; ITI-HC3; SHAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220252 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTTGCATGGTGGCCCTGTCTCATCTTGGCTCTGCTCTCCAGCTTGGCAGCCTCTGGCTTCCCGA
  GAAGCCCCTTTCGGCTGCTTGGGAAACGGAGCCTCCCGGAAGGGGTGGCCAATGGCATCGAGGTCTACAG
  TACCAAAATCAACTCCAAGGTGACCTCCCGTTTTGCTCACAATGTTGTCACCATGAGAGCCGTCAACCGT
  GCAGACACGGCCAAGGAGGTTTCCTTTGATGTGGAGCTGCCCAAGACGGCCTTCATCACCAACTTCACCT
  TGACCATCGACGGTGTTACCTACCCTGGGAATGTCAAGGAGAAGGAAGTTGCCAAGAAGCAGTATGAAAA
  GGCTGTGTCCCAGGGCAAGACGGCCGGCTTGGTCAAGGCCTCTGGGAGGAAGTTGGAGAAGTTCACAGTC
  TCGGTCAACGTGGCTGCAGGCAGCAAAGTCACCTTCGAGCTAACCTACGAGGAGCTGCTGAAGAGGCACA
  AGGGCAAGTACGAGATGTACCTCAAGGTCCAGCCTAAGCAACTGGTCAAACACTTTGAGATCGAGGTAGA
  CATCTTCGAGCCTCAGGGAATCAGCATGCTGGATGCTGAGGCCTCTTTCATCACCAACGACCTCCTGGGA
  AGCGCCCTCACCAAGTCCTTCTCAGGGAAAAAGGGCCATGTGTCCTTCAAGCCCAGCTTAGACCAACAGC
  GTTCATGCCCAACCTGTACAGACTCCCTCCTCAATGGAGATTTCACTATCACCTATGACGTGAACAGAGA
  ATCTCCTGGCAACGTGCAGATAGTCAATGGCTACTTCGTGCACTTCTTTGCACCTCAAGGCCTTCCAGTG
  GTGCCTAAGAACGTGGCCTTTGTGATTGACATCAGCGGCTCCATGGCTGGTCGGAAATTAGAGCAGACAA
  AGGAGGCCCTTCTCAGAATCCTGGAAGATATGCAAGAGGAAGACTATCTGAATTTCATCCTGTTCAGTGG
  AGATGTGTCCACATGGAAAGAGCACTTAGTCCAGGCCACGCCCGAGAACCTCCAGGAGGCCAGGACGTTT
  GTGAAGAGCATGGAGGATAAAGGAATGACCAACATCAATGACGGGCTGCTGAGGGGCATCAGTATGCTGA
  ACAAGGCCCGAGAGGAGCACAGAATCCCAGAGAGGAGCACCTCCATTGTCATCATGCTGACTGATGGGGA
  TGCCAATGTTGGTGAGAGCAGACCCGAAAAAATCCAAGAGAATGTGCGGAATGCCATCGGGGGCAAGTTC
  CCCTTGTATAACCTGGGCTTTGGCAACAATCTGAATTATAACTTCCTGGAGAACATGGCCCTGGAGAACC
  ATGGGTTTGCCCGGCGCATTTATGAGGACTCTGATGCCGATTTGCAGTTGCAGGGCTTCTATGAGGAGGT
  GGCCAACCCACTGCTGACGGGTGTGGAGATGGAGTACCCCGAGAACGCTATCCTGGACCTCACCCAGAAC
  ACTTACCAGCACTTCTACGATGGCTCTGAGATCGTGGTGGCCGGGCGCCTGGTGGACGAGGACATGAACA
  GCTTTAAGGCAGATGTGAAGGGCCATGGGGCCACCAACGACCTGACCTTCACAGAGGAGGTGGACATGAA
  GGAGATGGAGAAGGCCCTGCAGGAGCGGGACTACATCTTCGGGAATTACATTGAGCGGCTCTGGGCCTAC
  CTCACCATTGAGCAGCTGCTGGAGAAGCGCAAGAACGCCCATGGCGAGGAGAAGGAGAACCTCACGGCCC
  GGGCCCTGGACCTGTCCCTCAAGTATCACTTTGTGACTCCACTGACCTCAATGGTGGTGACCAAGCCTGA
  GGACAACGAGGATGAGAGGGCCATTGCCGACAAGCCTGGGGAAGATGCAGAAGCCACACCGGTGAGCCCC
  GCCATGTCCTACCTGACCAGCTACCAGCCTCCTCAAAACCCCTACTACTATGTGGACGGGGATCCCCACT
  TCATCATCCAAATTCCGGAGAAAGACGATGCCCTCTGCTTCAACATCGATGAAGCCCCAGGCACAGTGCT
  GCGCCTTATTCAGGATGCAGTCACAGGCCTCACAGTTAATGGGCAGATCACTGGCGACAAGAGAGGCAGC
  CCTGACTCCAAGACCAGAAAGACTTACTTTGGAAAACTGGGCATCGCCAATGCTCAGATGGACTTCCAGG
  TGGAGGTGACAACGGAGAAGATCACCCTGTGGAACAGGGCCGTGCCGAGCACTTTCAGCTGGCTGGACAC
  AGTCACAGTCACGCAGGATGGGCTGTCCATGATGATCAACAGGAAGAACATGGTGGTCTCCTTTGGAGAT
  GGGGTTACCTTCGTGGTCGTCCTACACCAGGTGTGGAAGAAACATCCTGTCCACCGTGACTTTCTAGGCT
  TCTACGTGGTGGACAGTCACCGGATGTCAGCACAGACGCATGGGCTGCTGGGGCAATTCTTCCAACCCTT
  TGACTTTAAAGTGTCTGACATCCGGCCAGGCTCTGACCCCACAAAGCCAGATGCCACATTGGTGGTGAAG
  AACCATCAGCTGATTGTCACCAGGGGCTCCCAGAAAGACTACAGAAAGGATGCCAGCATCGGCACGAAGG
  TTGTCTGCTGGTTCGTCCACAACAACGGAGAAGGGCTGATTGATGGTGTCCACACTGACTACATTGTCCC
  CAACCTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220252 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFAWWPCLILALLSSLAASGFPRSPFRLLGKRSLPEGVANGIEVYSTKINSKVTSRFAHNVVTMRAVNR
  ADTAKEVSFDVELPKTAFITNFTLTIDGVTYPGNVKEKEVAKKQYEKAVSQGKTAGLVKASGRKLEKFTV
  SVNVAAGSKVTFELTYEELLKRHKGKYEMYLKVQPKQLVKHFEIEVDIFEPQGISMLDAEASFITNDLLG
  SALTKSFSGKKGHVSFKPSLDQQRSCPTCTDSLLNGDFTITYDVNRESPGNVQIVNGYFVHFFAPQGLPV
  VPKNVAFVIDISGSMAGRKLEQTKEALLRILEDMQEEDYLNFILFSGDVSTWKEHLVQATPENLQEARTF
  VKSMEDKGMTNINDGLLRGISMLNKAREEHRIPERSTSIVIMLTDGDANVGESRPEKIQENVRNAIGGKF
  PLYNLGFGNNLNYNFLENMALENHGFARRIYEDSDADLQLQGFYEEVANPLLTGVEMEYPENAILDLTQN
  TYQHFYDGSEIVVAGRLVDEDMNSFKADVKGHGATNDLTFTEEVDMKEMEKALQERDYIFGNYIERLWAY
  LTIEQLLEKRKNAHGEEKENLTARALDLSLKYHFVTPLTSMVVTKPEDNEDERAIADKPGEDAEATPVSP
  AMSYLTSYQPPQNPYYYVDGDPHFIIQIPEKDDALCFNIDEAPGTVLRLIQDAVTGLTVNGQITGDKRGS
  PDSKTRKTYFGKLGIANAQMDFQVEVTTEKITLWNRAVPSTFSWLDTVTVTQDGLSMMINRKNMVVSFGD
  GVTFVVVLHQVWKKHPVHRDFLGFYVVDSHRMSAQTHGLLGQFFQPFDFKVSDIRPGSDPTKPDATLVVK
  NHQLIVTRGSQKDYRKDASIGTKVVCWFVHNNGEGLIDGVHTDYIVPNLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002217
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002217.3, NP_002208.3
• RefSeq Size: 3037 bp
• RefSeq ORF: 2673 bp
• Locus ID: 3699
• UniProt ID: Q06033
• Cytogenetics: 3p21.1
• Domains: VWA, VIT
• MW: 99.8 kDa
• Gene Summary: This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3. [provided by RefSeq, Jul 2015]