splicing factor 1 (SF1) (NM_004630) Human Tagged ORF Clone

CAT#: RC219995

SF1 (Myc-DDK-tagged)-Human splicing factor 1 (SF1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417858) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219995 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SF1
• Synonyms: BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219995 representing NM_004630
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGGAGCGAACGCCACGCCGTTGGACTTCCCAAGTAAGAAGCGGAAGAGGAGCCGCTGGAACC
  AAGACACAATGGAACAGAAGACAGTGATTCCAGGAATGCCTACAGTTATTCCCCCTGGACTTACTCGAGA
  ACAAGAAAGAGCTTATATAGTGCAACTGCAGATAGAAGACCTGACTCGTAAACTGCGCACAGGAGACCTG
  GGCATCCCCCCTAACCCTGAGGACAGGTCCCCTTCCCCTGAGCCCATCTACAATAGCGAGGGGAAGCGGC
  TTAACACCCGAGAGTTCCGCACCCGCAAAAAGCTGGAAGAGGAGCGGCACAACCTCATCACAGAGATGGT
  TGCACTCAATCCGGATTTCAAGCCACCTGCAGATTACAAACCTCCAGCAACACGTGTGAGTGATAAAGTC
  ATGATTCCACAAGATGAGTACCCAGAAATCAACTTTGTGGGGCTGCTCATCGGGCCCAGAGGGAACACCC
  TGAAGAACATAGAGAAGGAGTGCAATGCCAAGATTATGATCCGGGGGAAAGGGTCTGTGAAAGAAGGGAA
  GGTTGGGCGCAAAGATGGCCAGATGTTGCCAGGAGAAGATGAGCCACTTCATGCCCTGGTTACTGCCAAT
  ACAATGGAGAACGTCAAAAAGGCAGTGGAACAGATAAGAAACATCCTGAAGCAGGGTATCGAGACTCCAG
  AGGACCAGAATGATCTACGGAAGATGCAGCTTCGGGAGTTGGCTCGCTTAAATGGGACCCTTCGGGAAGA
  CGATAACAGGATCTTAAGACCCTGGCAGAGCTCAGAGACCCGCAGCATTACCAACACCACAGTGTGTACC
  AAGTGTGGAGGGGCTGGCCACATTGCTTCAGACTGTAAATTCCAAAGGCCTGGTGATCCTCAGTCAGCTC
  AGGATAAAGCACGGATGGATAAAGAATATTTGTCCCTCATGGCTGAACTGGGTGAAGCACCTGTCCCAGC
  ATCTGTGGGCTCCACCTCTGGGCCTGCCACCACACCCCTGGCCAGCGCACCTCGTCCTGCTGCTCCCGCC
  AACAACCCACCTCCACCGTCTCTCATGTCTACCACCCAGAGCCGCCCACCCTGGATGAATTCTGGCCCTT
  CAGAGAGTCGGCCCTACCACGGCATGCATGGAGGTGGTCCTGGTGGGCCCGGAGGTGGCCCCCACAGCTT
  CCCACACCCATTACCCAGCCTGACAGGTGGGCATGGTGGACATCCCATGCAGCACAACCCCAATGGACCC
  CCACCCCCTTGGATGCAGCCACCACCACCACCGATGAACCAGGGCCCCCACCCTCCTGGGCACCATGGCC
  CTCCTCCAATGGATCAGTACCTGGGAAGTACGCCTGTGGGCTCTGGGGTCTATCGCCTGCATCAAGGAAA
  AGGTATGATGCCGCCACCACCTATGGGCATGATGCCGCCGCCGCCGCCGCCTCCCAGTGGGCAGCCCCCA
  CCCCCTCCCTCTGGTCCTCTTCCCCCATGGCAACAACAGCAGCAGCAGCCTCCGCCACCCCCTCCGCCCA
  GCAGCAGTATGGCTTCCAGTACCCCCTTGCCATGGCAGCAAAATACGACGACTACCACCACGAGCGCTGG
  CACAGGGTCCATCCCGCCATGGCAACAGCAGCAGGCGGCTGCCGCAGCTTCTCCAGGAGCCCCTCAGATG
  CAAGGCAACCCCACTATGGTGCCCCTGCCCCCCGGGGTCCAGCCGCCTCTGCCGCCTGGGGCCCCTCCCC
  CTCCGCCGCCTCCACCGCCTGGTTCCGCCGGCATGATGTATGCCCCGCCCCCTCCTCCTCCGCCTCCCAT
  GGACCCTTCTAACTTTGTCACCATGATGGGCATGGGGGTGGCGGGCATGCCGCCCTTCGGGATGCCTCCA
  GCTCCCCCACCGCCTCCACCACAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219995 representing NM_004630
  Red=Cloning site Green=Tags(s)
  
  MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDL
  GIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKV
  MIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTAN
  TMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCT
  KCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPA
  NNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGP
  PPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPP
  PPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQM
  QGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPP
  APPPPPPQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004630
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004630.4
• RefSeq Size: 3527 bp
• RefSeq ORF: 1920 bp
• Locus ID: 7536
• UniProt ID: Q15637
• Cytogenetics: 11q13.1
• Domains: zf-CCHC, KH
• Protein Families: Transcription Factors
• MW: 68.1 kDa
• Gene Summary: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]