MARCHF11 (NM_001102562) Human Tagged ORF Clone

CAT#: RC219988

MARCH11 (Myc-DDK-tagged)-Human membrane-associated ring finger 11 (MARCH11)

Product Images

Circular map for RC219988

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MARCHF11
• Synonyms: MARCH-XI; MARCH11; RNF226
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219988 representing NM_001102562
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTTGAGGGCGGCCACGGCGGCAGTCGGTGTCGCGGGGCGGAGAGCGGGGACGCCGAGCCTCCCC
  CGCAACCTCCCCCGCCGCCGCCGCCGACGCCGCCGCCGGGAGAGCCGGCCCCGGTCCCCGCGGCCCCGCG
  CTACCTGCCGCCGCTGCCCGCGTCCCCCGAGACCCCCGAGCGCGCCGCGGGGCCAAGCGAGCCGCTAGGG
  GAGGTGGCCCCGCGGTGCAGGGGAGCGGACGAGCTGCCGCCTCCGCCCCTGCCCCTGCAGCCCGCCGGCC
  AGGAAGTGGCGGCGGCCGGCGACTCCGGGGAAGGTCCGAGGCGCCTCCCGGAGGCGGCAGCAGCGAAAGG
  CGGCCCCGGGGAGTCTGAGGCCGGCGCGGGCGGCGAGCGCGAGCGGCGGGGCGCCGGAGACCAGCCCGAG
  ACGCGCTCGGTGTGCAGCAGCCGCAGCAGCAGCAGTGGCGGCGGCGACCAGCGCGCTGGGCACCAGCACC
  AGCACCACCAGCCCATCTGCAAGATCTGCTTCCAGGGCGCGGAGCAGGGTGAGTTGTTGAACCCCTGCCG
  ATGTGATGGGTCAGTTCGGTATACACATCAGCTGTGCCTGCTAAAATGGATCAGTGAGAGAGGTTCCTGG
  ACCTGTGAACTTTGCTGTTATAGATACCATGTTATAGCCATTAAAATGAAACAACCTTGCCAGTGGCAGA
  GCATTTCTATAACACTGGTTGAGAAAGTTCAGATGATTGCTGTAATCCTAGGATCCCTGTTCTTAATAGC
  CAGTGTGACTTGGCTCCTCTGGTCAGCCTTCAGCCCCTATGCAGTGTGGCAGAGGAAGGACATCCTTTTT
  CAGATCTGCTATGGAATGTATGGTTTTATGGATCTAGTGTGCATAGGTCTCATTGTTCATGAAGGAGCTG
  CAGTTTACAGAGTGTTTAAGCGCTGGCGAGCTGTGAATTTGCACTGGGATGTGTTAAATTATGACAAAGC
  CACAGACATCGAAGAAAGCAGCCGGGGAGAGTCTTCCACAAGTAGGACTTTGTGGTTGCCATTGACAGCT
  CTGCGGAACAGAAACTTGGTCCACCCAACTCAGTTAACCTCACCAAGGTTTCAGTGTGGCTATGTGTTAT
  TGCACCTGTTCAATCGGATGAGGCCCCATGAAGACTTATCAGAAGATAACAGCTCGGGGGAGGTTGTGAT
  GAGAGTGACTTCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219988 representing NM_001102562
  Red=Cloning site Green=Tags(s)
  
  MSFEGGHGGSRCRGAESGDAEPPPQPPPPPPPTPPPGEPAPVPAAPRYLPPLPASPETPERAAGPSEPLG
  EVAPRCRGADELPPPPLPLQPAGQEVAAAGDSGEGPRRLPEAAAAKGGPGESEAGAGGERERRGAGDQPE
  TRSVCSSRSSSSGGGDQRAGHQHQHHQPICKICFQGAEQGELLNPCRCDGSVRYTHQLCLLKWISERGSW
  TCELCCYRYHVIAIKMKQPCQWQSISITLVEKVQMIAVILGSLFLIASVTWLLWSAFSPYAVWQRKDILF
  QICYGMYGFMDLVCIGLIVHEGAAVYRVFKRWRAVNLHWDVLNYDKATDIEESSRGESSTSRTLWLPLTA
  LRNRNLVHPTQLTSPRFQCGYVLLHLFNRMRPHEDLSEDNSSGEVVMRVTSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102562
• ORF Size: 1206 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102562.3
• RefSeq Size: 1528 bp
• RefSeq ORF: 1209 bp
• Locus ID: 441061
• UniProt ID: A6NNE9
• Cytogenetics: 5p15.1
• MW: 43.7 kDa
• Gene Summary: MARCH11 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). These enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their intracellular transport. March11 appears to have a role in ubiquitin-mediated protein sorting in the trans-Golgi network (TGN)-multivesicular body (MVB) transport pathway (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]