UNC119B (NM_001080533) Human Tagged ORF Clone

CAT#: RC219902

UNC119B (Myc-DDK-tagged)-Human unc-119 homolog B (C. elegans) (UNC119B)

Product Images

Circular map for RC219902

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  Western blot validation of overexpression lysate (Cat# LY421088) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219902 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UNC119B protein (Cat# TP319902). The protein was produced from HEK293T cells transfected with UNC119B cDNA clone (Cat# RC219902) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UNC119B
• Synonyms: POC7B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219902 representing NM_001080533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGTCTAACCCGAAGGCTGCGGCCGCGGCGTCGGCGGCTGGGCCCGGGGGGCTGGTGGCTGGCA
  AGGAGGAGAAGAAGAAGGCGGGCGGCGGCGTCCTGAACCGCCTGAAGGCGCGGCGGCAGGCGCCCCACCA
  CGCGGCCGACGACGGCGTCGGGGCAGCGGTCACGGAGCAGGAGCTGCTGGCGCTGGACACCATCCGGCCC
  GAGCACGTCCTGCGCCTCAGCCGGGTCACCGAGAATTATTTATGTAAACCCGAAGACAACATCTACAGTA
  TTGATTTCACCCGCTTCAAAATTCGAGATTTGGAGACAGGGACAGTACTTTTTGAGATTGCCAAACCTTG
  CGTTTCAGACCAGGAGGAGGATGAGGAGGAGGGAGGTGGAGACGTGGACATCAGCGCAGGACGTTTTGTC
  CGCTATCAGTTCACACCGGCATTTCTCCGCCTCCGGACAGTCGGGGCTACGGTGGAGTTCACAGTGGGAG
  ACAAACCTGTTTCAAACTTCCGGATGATCGAACGGCACTATTTCCGGGAACACTTGCTGAAAAACTTTGA
  CTTTGATTTTGGCTTCTGCATCCCCAGCAGTAGGAACACTTGTGAACATATCTATGAGTTTCCCCAGCTT
  TCGGAGGATGTCATTCGTCTAATGATTGAAAATCCTTACGAGACCCGCTCTGACAGCTTCTACTTTGTTG
  ACAACAAGCTGATAATGCACAACAAGGCTGATTATGCCTATAATGGAGGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219902 representing NM_001080533
  Red=Cloning site Green=Tags(s)
  
  MSGSNPKAAAAASAAGPGGLVAGKEEKKKAGGGVLNRLKARRQAPHHAADDGVGAAVTEQELLALDTIRP
  EHVLRLSRVTENYLCKPEDNIYSIDFTRFKIRDLETGTVLFEIAKPCVSDQEEDEEEGGGDVDISAGRFV
  RYQFTPAFLRLRTVGATVEFTVGDKPVSNFRMIERHYFREHLLKNFDFDFGFCIPSSRNTCEHIYEFPQL
  SEDVIRLMIENPYETRSDSFYFVDNKLIMHNKADYAYNGGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080533
• ORF Size: 753 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080533.3
• RefSeq Size: 4811 bp
• RefSeq ORF: 756 bp
• Locus ID: 84747
• UniProt ID: A6NIH7
• Cytogenetics: 12q24.31
• MW: 28 kDa
• Gene Summary: Myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated NPHP3 and plays a key role in localization of NPHP3 to the primary cilium membrane. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells.[UniProtKB/Swiss-Prot Function]