POLK (NM_016218) Human Tagged ORF Clone

CAT#: RC219806

POLK (Myc-DDK-tagged)-Human polymerase (DNA directed) kappa (POLK)

Product Images

Circular map for RC219806

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  Western blot validation of overexpression lysate (Cat# LY414115) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219806 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified POLK protein (Cat# TP319806). The protein was produced from HEK293T cells transfected with POLK cDNA clone (Cat# RC219806) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLK
• Synonyms: DINB1; DINP; POLQ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219806 representing NM_016218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCTGCTTAGGATGGGACTTAATGATA
  ATAAAGCAGGAATGGAAGGATTAGATAAAGAGAAAATTAACAAAATTATAATGGAAGCCACGAAGGGGTC
  CAGATTTTATGGAAATGAGCTCAAGAAAGAAAAGCAAGTCAACCAACGAATTGAAAATATGATGCAACAA
  AAAGCTCAAATCACCAGCCAACAGCTAAGAAAAGCACAATTACAGGTTGACAGATTTGCAATGGAATTAG
  AACAAAGCCGAAATTTGAGCAATACCATAGTGCACATTGACATGGATGCTTTCTATGCAGCTGTAGAAAT
  GAGGGACAATCCAGAATTGAAGGATAAACCCATTGCTGTAGGATCAATGAGTATGCTGTCTACTTCAAAT
  TACCATGCAAGGAGATTTGGTGTTCGTGCAGCCATGCCAGGATTTATTGCTAAGAGGCTGTGCCCACAAC
  TTATAATAGTGCCCCCCAACTTTGACAAATACCGAGCTGTGAGTAAAGAGGTTAAGGAAATACTTGCTGA
  TTATGATCCCAATTTTATGGCCATGAGTCTTGATGAAGCCTACTTGAATATAACAAAGCACTTAGAAGAA
  AGACAAAATTGGCCTGAGGATAAAAGAAGGTATTTCATCAAAATGGGAAGCTCTGTAGAAAATGATAATC
  CAGGAAAGGAAGTTAATAAACTGAGTGAGCATGAACGATCCATCTCTCCACTACTTTTTGAAGAGAGTCC
  TTCTGATGTGCAGCCCCCAGGAGATCCTTTCCAAGTGAACTTTGAAGAACAAAACAATCCTCAAATACTC
  CAAAACTCAGTTGTTTTTGGAACATCAGCCCAGGAAGTGGTAAAGGAAATTCGTTTCAGAATTGAGCAGA
  AAACAACACTGACAGCTAGTGCAGGCATTGCCCCAAATACAATGTTAGCAAAAGTATGCAGTGATAAGAA
  TAAACCAAATGGACAATACCAAATTCTTCCCAATAGACAAGCTGTGATGGACTTCATCAAGGATTTACCC
  ATTAGAAAGGTTTCTGGAATAGGAAAAGTTACAGAGAAAATGTTAAAGGCCCTTGGAATTATTACATGTA
  CAGAACTTTACCAACAGAGGGCATTGCTTTCTCTCCTTTTCTCTGAAACATCTTGGCATTATTTCCTTCA
  TATCTCCTTGGGTCTAGGTTCAACACACCTGACGAGGGATGGAGAGAGGAAAAGTATGAGCGTTGAGAGG
  ACATTCAGTGAGATAAATAAAGCGGAAGAGCAATACAGCCTATGTCAAGAACTTTGCAGTGAGCTTGCTC
  AGGATCTACAGAAAGAAAGACTTAAGGGTAGAACTGTTACCATTAAGTTGAAGAATGTGAATTTTGAAGT
  AAAAACTCGTGCATCTACAGTTTCATCTGTTGTTTCTACTGCAGAAGAAATATTTGCCATTGCTAAGGAA
  TTGCTAAAAACAGAAATTGATGCTGATTTTCCACATCCCTTGAGATTAAGGCTTATGGGTGTTCGGATAT
  CTAGTTTTCCCAATGAAGAGGACAGGAAACACCAACAAAGGAGCATTATTGGCTTTTTACAGGCTGGAAA
  CCAAGCCCTGTCAGCCACTGAGTGTACATTAGAGAAAACTGACAAAGATAAGTTTGTAAAACCTCTAGAA
  ATGTCTCATAAGAAGAGTTTCTTTGATAAAAAACGATCAGAAAGGAAATGGAGTCACCAAGATACATTTA
  AATGTGAAGCCGTGAATAAACAAAGTTTCCAGACATCACAACCATTCCAAGTTTTAAAGAAGAAGATGAA
  TGAGAATTTGGAAATATCAGAGAATTCAGATGACTGTCAGATACTTACCTGTCCTGTTTGCTTTAGGGCT
  CAAGGGTGCATCAGTCTGGAAGCCTTGAATAAACATGTAGATGAATGTCTTGATGGACCTTCAATCAGTG
  AAAACTTTAAAATGTTCTCGTGTTCACATGTTTCTGCTACCAAAGTTAACAAGAAAGAAAATGTTCCTGC
  TTCTTCACTTTGTGAGAAGCAAGATTATGAAGCCCATCCAAAAATTAAAGAAATATCTTCAGTAGATTGT
  ATAGCTTTAGTAGATACTATAGATAACTCATCTAAAGCAGAAAGCATAGATGCTTTAAGTAATAAGCATA
  GCAAGGAAGAATGTTCTAGTCTCCCAAGCAAGTCTTTTAATATTGAACACTGTCATCAGAATTCTTCTTC
  TACTGTTTCATTGGAAAACGAAGATGTTGGATCATTTAGACAAGAATACCGCCAGCCTTACTTATGTGAA
  GTGAAAACAGGCCAAGCTCTAGTTTGTCCTGTTTGTAACGTAGAACAAAAGACTTCAGATCTAACCCTGT
  TCAATGTGCATGTGGATGTTTGCTTAAATAAAAGTTTTATCCAAGAATTAAGAAAGGATAAATTTAACCC
  AGTTAATCAACCCAAAGAAAGCTCCAGAAGTACTGGTAGCTCAAGTGGAGTACAGAAGGCTGTAACAAGA
  ACAAAAAGGCCAGGATTGATGACAAAGTACTCAACATCAAAGAAAATAAAACCAAACAATCCCAAACATA
  CCCTTGATATATTTTTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219806 representing NM_016218
  Red=Cloning site Green=Tags(s)
  
  MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQ
  KAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSN
  YHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEE
  RQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQIL
  QNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLP
  IRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVER
  TFSEINKAEEQYSLCQELCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAKE
  LLKTEIDADFPHPLRLRLMGVRISSFPNEEDRKHQQRSIIGFLQAGNQALSATECTLEKTDKDKFVKPLE
  MSHKKSFFDKKRSERKWSHQDTFKCEAVNKQSFQTSQPFQVLKKKMNENLEISENSDDCQILTCPVCFRA
  QGCISLEALNKHVDECLDGPSISENFKMFSCSHVSATKVNKKENVPASSLCEKQDYEAHPKIKEISSVDC
  IALVDTIDNSSKAESIDALSNKHSKEECSSLPSKSFNIEHCHQNSSSTVSLENEDVGSFRQEYRQPYLCE
  VKTGQALVCPVCNVEQKTSDLTLFNVHVDVCLNKSFIQELRKDKFNPVNQPKESSRSTGSSSGVQKAVTR
  TKRPGLMTKYSTSKKIKPNNPKHTLDIFFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016218
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016218.3
• RefSeq Size: 4074 bp
• RefSeq ORF: 2613 bp
• Locus ID: 51426
• UniProt ID: Q9UBT6
• Cytogenetics: 5q13.3
• Domains: IMS, ZnF_Rad18
• Protein Families: Druggable Genome
• MW: 98.6 kDa
• Gene Summary: This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]