Factor XIII (F13B) (NM_001994) Human Tagged ORF Clone

CAT#: RC219609

F13B (Myc-DDK-tagged)-Human coagulation factor XIII, B polypeptide (F13B)

Product Images

Circular map for RC219609

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY F13B (Cat# RC219609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-F13B(Cat# TA805990). Positive lysates LY419598 (100ug) and LC419598 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY419598) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219609 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Factor XIII
• Synonyms: FXIIIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219609 representing NM_001994
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTTGAAAAACCTGACTTTTATCATCATATTGATAATCTCAGGAGAACTCTATGCAGAAGAGAAAC
  CCTGTGGTTTTCCTCATGTGGAAAATGGAAGAATTGCCCAATATTACTATACTTTTAAAAGCTTTTACTT
  TCCAATGAGCATAGACAAAAAATTGTCATTTTTCTGCTTGGCTGGTTATACCACTGAAAGTGGAAGACAA
  GAAGAGCAAACCACGTGTACAACAGAAGGCTGGTCTCCAGAGCCAAGGTGCTTCAAAAAATGCACTAAGC
  CTGACCTGAGTAATGGTTACATCTCTGATGTAAAGTTATTGTATAAAATTCAAGAGAACATGCGTTATGG
  TTGCGCTTCAGGGTACAAAACCACTGGAGGGAAGGATGAAGAAGTGGTTCAATGTCTCTCTGATGGATGG
  TCTTCTCAACCAACCTGTAGGAAAGAACATGAAACATGTTTGGCTCCTGAATTATATAATGGAAATTATT
  CCACAACACAGAAAACATTCAAAGTGAAGGACAAAGTACAATACGAATGTGCTACTGGCTACTACACAGC
  TGGAGGAAAGAAGACAGAGGAGGTAGAATGTCTCACATACGGATGGTCTCTCACACCAAAATGTACCAAA
  TTAAAGTGCTCTTCTTTAAGATTAATTGAAAATGGTTATTTTCATCCTGTAAAGCAAACCTATGAAGAAG
  GAGATGTCGTTCAGTTTTTCTGTCATGAAAATTATTATCTAAGTGGATCTGATTTAATTCAATGCTATAA
  CTTTGGTTGGTACCCAGAATCTCCTGTATGCGAAGGAAGAAGAAACAGATGTCCTCCTCCACCTCTGCCC
  ATAAACTCCAAAATTCAAACACATTCAACAACTTATCGTCATGGAGAAATAGTTCATATAGAATGTGAAC
  TTAATTTTGAGATCCATGGGTCAGCAGAAATACGTTGTGAAGATGGAAAATGGACAGAACCTCCAAAATG
  CATTGAAGGACAGGAGAAGGTAGCCTGTGAGGAACCACCCTTCATTGAAAATGGTGCAGCAAATTTACAC
  TCTAAGATTTATTACAATGGGGATAAAGTGACATATGCATGTAAAAGCGGCTACCTTCTCCATGGATCGA
  ATGAGATAACTTGTAATCGTGGAAAATGGACACTTCCTCCTGAGTGTGTTGAAAATAATGAGAATTGTAA
  GCATCCTCCTGTTGTAATGAATGGGGCTGTTGCAGACGGGATATTGGCAAGCTATGCAACAGGATCCTCA
  GTGGAATATAGATGCAATGAATATTACTTACTGAGGGGATCAAAAATATCTCGTTGCGAACAAGGAAAAT
  GGTCATCCCCACCTGTTTGCTTGGAACCATGTACTGTTAATGTGGATTACATGAACAGAAATAACATAGA
  AATGAAGTGGAAATATGAAGGGAAAGTCTTACATGGAGATTTAATAGATTTTGTATGTAAACAGGGATAT
  GACTTATCTCCATTAACCCCATTGTCTGAATTATCTGTGCAGTGCAACAGAGGAGAAGTGAAATATCCTT
  TATGTACTAGAAAAGAATCTAAAGGAATGTGCACATCTCCTCCTCTTATTAAACATGGAGTCATTATTAG
  TTCAACAGTAGACACCTATGAAAATGGCTCTTCAGTAGAATACAGATGTTTTGATCACCATTTCCTAGAA
  GGATCTAGGGAGGCCTATTGTTTAGATGGAATGTGGACTACACCACCATTGTGTTTAGAGCCATGCACAT
  TATCTTTTACTGAAATGGAAAAGAATAATTTACTTCTGAAATGGGATTTTGACAATAGACCACACATTTT
  GCATGGTGAATATATTGAGTTTATTTGTAGAGGAGATACTTATCCAGCTGAATTATATATTACTGGATCT
  ATACTTAGAATGCAATGTGACAGAGGGCAGTTAAAATATCCAAGATGTATTCCAAGACAAAGCACTCTGT
  CTTATCAAGAACCCTTAAGAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219609 representing NM_001994
  Red=Cloning site Green=Tags(s)
  
  MRLKNLTFIIILIISGELYAEEKPCGFPHVENGRIAQYYYTFKSFYFPMSIDKKLSFFCLAGYTTESGRQ
  EEQTTCTTEGWSPEPRCFKKCTKPDLSNGYISDVKLLYKIQENMRYGCASGYKTTGGKDEEVVQCLSDGW
  SSQPTCRKEHETCLAPELYNGNYSTTQKTFKVKDKVQYECATGYYTAGGKKTEEVECLTYGWSLTPKCTK
  LKCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPVCEGRRNRCPPPPLP
  INSKIQTHSTTYRHGEIVHIECELNFEIHGSAEIRCEDGKWTEPPKCIEGQEKVACEEPPFIENGAANLH
  SKIYYNGDKVTYACKSGYLLHGSNEITCNRGKWTLPPECVENNENCKHPPVVMNGAVADGILASYATGSS
  VEYRCNEYYLLRGSKISRCEQGKWSSPPVCLEPCTVNVDYMNRNNIEMKWKYEGKVLHGDLIDFVCKQGY
  DLSPLTPLSELSVQCNRGEVKYPLCTRKESKGMCTSPPLIKHGVIISSTVDTYENGSSVEYRCFDHHFLE
  GSREAYCLDGMWTTPPLCLEPCTLSFTEMEKNNLLLKWDFDNRPHILHGEYIEFICRGDTYPAELYITGS
  ILRMQCDRGQLKYPRCIPRQSTLSYQEPLRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001994
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001994.3
• RefSeq Size: 2208 bp
• RefSeq ORF: 1986 bp
• Locus ID: 2165
• UniProt ID: P05160
• Cytogenetics: 1q31.3
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades
• MW: 75.51 kDa
• Gene Summary: This gene encodes coagulation factor XIII B subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]