Chromosome X open reading frame 6 (MAMLD1) (NM_005491) Human Tagged ORF Clone

CAT#: RC219597

MAMLD1 (Myc-DDK-tagged)-Human mastermind-like domain containing 1 (MAMLD1), transcript variant 2

Product Images

Circular map for RC219597

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  Western blot validation of overexpression lysate (Cat# LY417276) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219597 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAMLD1 protein (Cat# TP319597). The protein was produced from HEK293T cells transfected with MAMLD1 cDNA clone (Cat# RC219597) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chromosome X open reading frame 6
• Synonyms: CG1; CXorf6; F18; HYSP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219597 representing NM_005491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGACTGGAAAAGTCGGCTTGTAATCAAGAGCATGCTTCCCCATTTCGCCATGGTGGGAAATCGTC
  AGGAGCCCAGAAAGCTCCAGGAATCGGGAAAGAAGCCCTCGTGGATGGAGGAAGAAGATTTATCTTTTCT
  CTACAAGAGCAGCCCAGGAAGAAAGCATCAGGGAACTGTTAAGAGGAGACAAGAAGAAGACCACTTCCAG
  TTTCCAGACATGGCTGATGGGGGCTACCCTAATAAAATTAAGAGGCCTTGCCTTGAAGATGTCACCCTTG
  CAATGGGCCCAGGTGCTCATCCTAGTACTGCTTGTGCAGAACTGCAGGTCCCTCCATTGACAATAAATCC
  TAGCCCTGCGGCTATGGGAGTGGCTGGCCAGTCATTACTGCTGGAGAATAACCCTATGAATGGCAACATC
  ATGGGCTCACCATTTGTAGTACCACAGACTACAGAAGTGGGACTGAAAGGGCCCACTGTTCCTTACTATG
  AGAAAATCAACAGCGTGCCGGCTGTAGACCAGGAGCTTCAAGAGCTGCTAGAGGAGCTCACCAAAATTCA
  AGACCCTTCTCCAAATGAGCTAGATCTTGAGAAGATACTGGGGACGAAGCCAGAAGAGCCACTGGTTTTA
  GATCATCCCCAGGCAACCCTAAGCACAACTCCCAAGCCTTCGGTTCAGATGTCACACTTGGAGAGCCTGG
  CTTCCAGCAAGGAGTTTGCTTCTAGTTGCAGCCAAGTTACTGGCATGTCACTTCAGATCCCATCCTCCTC
  CACAGGGATCAGCTATTCGATTCCTTCCACCAGTAAGCAGATAGTGTCACCGAGTTCTTCAATGGCACAG
  TCCAAGAGCCAGGTCCAGGCCATGCTCCCTGTCGCTCTGCCCCCCTTACCAGTGCCTCAGTGGCATCACG
  CCCACCAGCTGAAGGCGTTGGCAGCCAGCAAGCAGGGGTCTGCTACAAAGCAGCAAGGGCCCACCCCCAG
  TTGGTCTGGTCTGCCTCCTCCAGGACTCTCTCCACCTTACCGCCCAGTGCCATCACCACACCCACCACCG
  CTGCCACTGCCACCACCACCACCCCCATTCAGCCCCCAGAGCCTCATGGTGTCCTGCATGTCGTCCAATA
  CCTTGTCGGGTAGCACTCTCCGAGGCTCTCCCAATGCCTTACTGTCAAGCATGACGTCCAGCAGCAATGC
  TGCCCTGGGGCCCGCCATGCCCTATGCTCCTGAGAAGCTCCCCAGCCCTGCTCTCACTCAACAGCCGCAG
  TTCGGCCCTCAGAGCTCCATTCTTGCCAACCTCATGTCCTCTACCATCAAAACCCCTCAAGGACACCTGA
  TGTCTGCTCTGCCTGCCAGCAACCCTGGGCCGTCCCCACCCTATCGCCCAGAGAAGCTCTCTAGCCCAGG
  CTTGCCACAGCAGTCCTTCACCCCACAGTGTTCCCTGATCCGAAGCCTCACTCCCACCAGTAATCTTCTA
  AGCCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAAGCAAATGTGATCTTTAAGCCCATAAGCAGCAACT
  CATCCAAAACCCTGAGCATGATCATGCAGCAGGGGATGGCAAGCTCCAGCCCAGGAGCCACGGAGCCATT
  TACTTTTGGCAACACCAAGCCCTTGTCCCATTTTGTTTCTGAGCCGGGTCCCCAGAAGATGCCCTCCATG
  CCTACCACCTCTAGGCAGCCTTCCCTGCTCCACTACCTGCAGCAGCCGACACCAACGCAGGCCTCCTCAG
  CCACTGCCTCCTCCACGGCCACTGCCACCTTGCAGCTGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCC
  TGACCATTCTTCATTCCTTCTGCAGCAGATGATGCAGCAACCCCAGCGTTTTCAGCGATCAGTGGCCTCA
  GATTCCATGCCTGCTCTGCCCAGACAGGGCTGTTGCCATCTGTTTGCATGGACTTCTGCAGCTAGCTCGG
  TGAAGCCCCAGCATCAACACGGGAACTCTTTCACTAGCAGGCAAGATCCTCAGCCTGGAGACGTGTCACC
  GTCTAACATTACTCATGTAGACAAAGCCTGCAAGCTTGGGGAAGCCAGGCACCCCCAGGTCAGCCTCGGG
  CGACAGCCCCCGTCCTGCCAGGCCCTGGGGAGTGAGTCCTTCCTGCCCGGCAGCTCCTTTGCTCATGAGC
  TGGCCCGAGTCACCTCCTCGTACAGCACCTCAGAGGCAGCGCCCTGGGGCAGCTGGGATCCGAAGGCCTG
  GAGGCAGGTGCCCGCTCCACTACTGCCTAGCTGCGACGCCACAGCAAGAGGAACAGAGATCAGGTCTTAT
  GGCAATGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219597 representing NM_005491
  Red=Cloning site Green=Tags(s)
  
  MDDWKSRLVIKSMLPHFAMVGNRQEPRKLQESGKKPSWMEEEDLSFLYKSSPGRKHQGTVKRRQEEDHFQ
  FPDMADGGYPNKIKRPCLEDVTLAMGPGAHPSTACAELQVPPLTINPSPAAMGVAGQSLLLENNPMNGNI
  MGSPFVVPQTTEVGLKGPTVPYYEKINSVPAVDQELQELLEELTKIQDPSPNELDLEKILGTKPEEPLVL
  DHPQATLSTTPKPSVQMSHLESLASSKEFASSCSQVTGMSLQIPSSSTGISYSIPSTSKQIVSPSSSMAQ
  SKSQVQAMLPVALPPLPVPQWHHAHQLKALAASKQGSATKQQGPTPSWSGLPPPGLSPPYRPVPSPHPPP
  LPLPPPPPPFSPQSLMVSCMSSNTLSGSTLRGSPNALLSSMTSSSNAALGPAMPYAPEKLPSPALTQQPQ
  FGPQSSILANLMSSTIKTPQGHLMSALPASNPGPSPPYRPEKLSSPGLPQQSFTPQCSLIRSLTPTSNLL
  SQQQQQQQQQQQANVIFKPISSNSSKTLSMIMQQGMASSSPGATEPFTFGNTKPLSHFVSEPGPQKMPSM
  PTTSRQPSLLHYLQQPTPTQASSATASSTATATLQLQQQQQQQQQQPDHSSFLLQQMMQQPQRFQRSVAS
  DSMPALPRQGCCHLFAWTSAASSVKPQHQHGNSFTSRQDPQPGDVSPSNITHVDKACKLGEARHPQVSLG
  RQPPSCQALGSESFLPGSSFAHELARVTSSYSTSEAAPWGSWDPKAWRQVPAPLLPSCDATARGTEIRSY
  GNDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005491
• ORF Size: 2322 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005491.4, NP_005482.2
• RefSeq Size: 4636 bp
• RefSeq ORF: 2325 bp
• Locus ID: 10046
• UniProt ID: Q13495
• Cytogenetics: Xq28
• MW: 83.1 kDa
• Gene Summary: This gene encodes a mastermind-like domain containing protein. This protein may function as a transcriptional co-activator. Mutations in this gene are the cause of X-linked hypospadias type 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]