ZNRF3 (NM_032173) Human Tagged ORF Clone

CAT#: RC219546

ZNRF3 (Myc-DDK-tagged)-Human zinc and ring finger 3 (ZNRF3), transcript variant 2

Product Images

Circular map for RC219546

• 

  Western blot validation of overexpression lysate (Cat# LY410300) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219546 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNRF3
• Synonyms: BK747E2.3; RNF203
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219546 representing NM_032173
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCACTGGGCCTATGTAATAACAATGACGAAGAGGACTTGTATGAATATGGCTGGGTAGGAGTGG
  TGAAGCTGGAACAGCCAGAATTGGACCCGAAACCATGCCTCACTGTCCTAGGCAAGGCCAAGCGAGCAGT
  ACAGCGGGGAGCTACTGCAGTCATCTTTGATGTGTCTGAAAACCCAGAAGCTATTGATCAGCTGAACCAG
  GGCTCTGAAGACCCGCTCAAGAGGCCGGTGGTGTATGTGAAGGGTGCAGATGCCATTAAGCTGATGAACA
  TCGTCAACAAGCAGAAAGTGGCTCGAGCAAGGATCCAGCACCGCCCTCCTCGACAACCCACTGAATACTT
  TGACATGGGGATTTTCCTGGCTTTCTTCGTCGTGGTCTCCTTGGTCTGCCTCATCCTCCTTGTCAAAATC
  AAGCTGAAGCAGCGACGCAGTCAGAATTCCATGAACAGGCTGGCTGTGCAGGCTCTAGAGAAGATGGAAA
  CCAGAAAGTTCAACTCCAAGAGCAAGGGGCGCCGGGAGGGGAGCTGTGGGGCCCTGGACACACTCAGCAG
  CAGCTCCACGTCCGACTGTGCCATCTGTCTGGAGAAGTACATTGATGGAGAGGAGCTGCGGGTCATCCCC
  TGTACTCACCGGTTTCACAGGAAGTGCGTGGACCCCTGGCTGCTGCAGCACCACACCTGCCCCCACTGTC
  GGCACAACATCATAGAACAAAAGGGAAACCCAAGCGCGGTGTGTGTGGAGACCAGCAACCTCTCACGTGG
  TCGGCAGCAGAGGGTGACCCTGCCGGTGCATTACCCCGGCCGCGTGCACAGGACCAACGCCATCCCAGCC
  TACCCTACGAGGACAAGCATGGACTCCCACGGCAACCCCGTCACCTTGCTGACCATGGACCGGCACGGGG
  AGCAGAGCCTCTATTCCCCGCAGACCCCCGCCTACATCCGCAGCTACCCACCCCTCCACCTGGACCACAG
  CCTGGCCGCTCACCGCTGCGGCCTGGAGCACCGGGCCTACTCCCCAGCCCACCCCTTCCGCAGGCCCAAG
  TTGAGTGGCCGCAGCTTCTCCAAGGCAGCTTGCTTCTCCCAGTATGAGACCATGTACCAGCACTACTACT
  TCCAGGGCCTCAGCTACCCGGAGCAGGAGGGGCAGTCCCCACCTAGCCTCGCACCCCGGGGCCCGGCCCG
  TGCCTTTCCTCCGAGCGGCAGTGGCAGCCTGCTCTTCCCCACCGTGGTGCACGTGGCCCCGCCCTCCCAC
  CTGGAGAGCGGCAGCACGTCCAGCTTCAGCTGCTATCACGGCCACCGCTCGGTGTGCAGTGGCTACCTGG
  CCGACTGCCCAGGCAGCGACAGCAGCAGCAGCAGCAGCTCCGGCCAGTGCCACTGTTCCTCCAGTGACTC
  TGTGGTAGACTGCACTGAGGTCAGCAACCAGGGCGTGTACGGGAGCTGCTCCACCTTCCGCAGCTCCCTC
  AGCAGCGACTATGACCCCTTCATCTACCGCAGCCGGAGCCCCTGTCGTGCCAGTGAGGCGGGGGGCTCGG
  GCAGCTCGGGCCGGGGACCTGCCCTGTGCTTCGAGGGCTCCCCGCCTCCCGAGGAGCTCCCGGCGGTGCA
  CAGTCATGGTGCTGGGCGGGGCGAGCCTTGGCCGGGCCCTGCCTCTCCCTCGGGGGATCAGGTGTCCACC
  TGCAGCCTGGAGATGAACTACAGCAGCAACTCCTCCCTGGAGCACAGGGGGCCCAATAGCTCTACCTCAG
  AAGTGGGGCTCGAGGCTTCTCCTGGGGCCGCCCCTGACCTCAGGAGGACCTGGAAGGGGGGCCACGAGTT
  GCCGTCGTGTGCCTGCTGCTGCGAGCCCCAGCCCTCCCCAGCCGGGCCTAGCGCCGGAGCAGCTGGCAGC
  AGCACCTTGTTCCTGGGGCCCCACCTCTACGAGGGCTCTGGCCCGGCGGGTGGGGAGCCCCAGTCAGGAA
  GCTCCCAGGGCTTGTACGGCCTTCACCCCGACCATTTGCCCAGGACAGATGGGGTGAAATACGAGGGTCT
  GCCCTGCTGCTTCTATGAAGAGAAGCAGGTGGCCCGCGGGGGCGGAGGGGGCAGCGGCTGCTACACTGAG
  GACTACTCGGTGAGTGTGCAGTACACGCTCACCGAGGAACCACCGCCCGGCTGCTACCCCGGGGCCCGGG
  ACCTGAGCCAGCGCATCCCCATCATTCCAGAGGATGTGGACTGTGATCTGGGCCTGCCCTCGGACTGCCA
  AGGGACCCACAGCCTCGGCTCCTGGGGTGGGACGCGAGGCCCGGATACCCCACGGCCCCACAGGGGCCTG
  GGAGCAACCCGGGAAGAGGAGCGGGCTCTGTGCTGCCAGGCTAGGGCCCTACTGCGGCCTGGCTGCCCTC
  CGGAGGAGGCGGGTGCTGTCAGGGCCAACTTCCCTAGTGCCCTCCAGGACACTCAGGAGTCCAGCACCAC
  TGCCACTGAGGCTGCAGGACCGAGATCTCACTCAGCAGACAGCAGCAGCCCGGGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219546 representing NM_032173
  Red=Cloning site Green=Tags(s)
  
  MHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLNQ
  GSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHRPPRQPTEYFDMGIFLAFFVVVSLVCLILLVKI
  KLKQRRSQNSMNRLAVQALEKMETRKFNSKSKGRREGSCGALDTLSSSSTSDCAICLEKYIDGEELRVIP
  CTHRFHRKCVDPWLLQHHTCPHCRHNIIEQKGNPSAVCVETSNLSRGRQQRVTLPVHYPGRVHRTNAIPA
  YPTRTSMDSHGNPVTLLTMDRHGEQSLYSPQTPAYIRSYPPLHLDHSLAAHRCGLEHRAYSPAHPFRRPK
  LSGRSFSKAACFSQYETMYQHYYFQGLSYPEQEGQSPPSLAPRGPARAFPPSGSGSLLFPTVVHVAPPSH
  LESGSTSSFSCYHGHRSVCSGYLADCPGSDSSSSSSSGQCHCSSSDSVVDCTEVSNQGVYGSCSTFRSSL
  SSDYDPFIYRSRSPCRASEAGGSGSSGRGPALCFEGSPPPEELPAVHSHGAGRGEPWPGPASPSGDQVST
  CSLEMNYSSNSSLEHRGPNSSTSEVGLEASPGAAPDLRRTWKGGHELPSCACCCEPQPSPAGPSAGAAGS
  STLFLGPHLYEGSGPAGGEPQSGSSQGLYGLHPDHLPRTDGVKYEGLPCCFYEEKQVARGGGGGSGCYTE
  DYSVSVQYTLTEEPPPGCYPGARDLSQRIPIIPEDVDCDLGLPSDCQGTHSLGSWGGTRGPDTPRPHRGL
  GATREEERALCCQARALLRPGCPPEEAGAVRANFPSALQDTQESSTTATEAAGPRSHSADSSSPGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032173
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032173.3, NP_115549.2
• RefSeq Size: 6541 bp
• RefSeq ORF: 2511 bp
• Locus ID: 84133
• UniProt ID: Q9ULT6
• Cytogenetics: 22q12.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 89.7 kDa
• Gene Summary: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone (PubMed:22575959). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).[UniProtKB/Swiss-Prot Function]