MFSD6 (NM_017694) Human Tagged ORF Clone

CAT#: RC219252

MFSD6 (Myc-DDK-tagged)-Human major facilitator superfamily domain containing 6 (MFSD6)

Product Images

Circular map for RC219252

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  Western blot validation of overexpression lysate (Cat# LY413606) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219252 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MFSD6 protein (Cat# TP319252). The protein was produced from HEK293T cells transfected with MFSD6 cDNA clone (Cat# RC219252) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MFSD6
• Synonyms: hMMR2; MMR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219252 representing NM_017694
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATGATAAAGTTGCTATCTTAACGGATGATGAAGAGGAACAGAAGAGAAAGTATGTGCTTGCAG
  ATCCCTTTAATGGTATTTCCAGGGAACCAGAACCACCTTCGAATGAAACACCTTCCTCCACAGAAACATC
  TGCTATTCCTGAGGAGGAAATAGACTGGATAGAGAAACATTGTGTTAAGATAAACAACGATCTTCTAATT
  TCCAAGGTCTTTTATTTTTTCTTTTACTCTGCCTATGGCTCTCTCTATCCCCTTTTGCCTGTGTATTACA
  AACAGCTGGGAATGTCTCCAAGCCAGAGTGGACTACTAGTAGGTATTCGTTACTTCATTGAATTCTGCAG
  TGCCCCCTTTTGGGGTGTAGTTGCAGACCGCTTTAAAAAAGGCAAAATTGTCCTCCTCTTTTCTCTTTTG
  TGTTGGGTTTTATTCAACCTGGGCATTGGATTTGTCAAACCTGCTACCTTGAGATGTGTACCAAAGATTC
  GCCCAACAACTCACCCCACCAATGCAAGTCACCAGTTAACTATCCTGCCAACAAATTCTTCCTTTACCTC
  TTTCCTCACCATATCACCAAAAATGCGTGAGAAAAGAAACCTTTTGGAAACAGGGCTCAATGTCTCAGAC
  ACCGTTACTTTGCCAACAGCTCCAAACATGAACAGTGAACCCACTCTGCAGCCCCAGACAGGTGAAATTA
  CTAACCGTATGATGGACTTGACTTTGAACTCAAGCACAGCAACCCCTGTCTCCCCAGGAAGCGTAACCAA
  GGAGACAACCACTGTTATTGTTACCACCACCAAATCTTTACCTTCTGACCAAGTCATGCTTGTTTATGAT
  CAACAAGAAGTTGAAGCTATATTCTTGGTGATCTTGGTAGTTGTCATAATAGGAGAATTTTTCAGTGCCT
  CTTCTGTCACAATCGTAGACACGGTCACACTCCAGTATCTGGGAAAACACAGAGATCGCTATGGGTTGCA
  GCGCATGTGGGGCTCCCTGGGCTGGGGCCTGGCGATGCTGTCTGTGGGCATCGGGATCGACTACACCCAC
  ATCGAAGTGCTCATCGATGGAAAGGGGTGTAAGCCCCCCGAGTACAGGAATTACCAGATCGTCTTCATCG
  TCTTCGGCGTTCTCATGACCATGGCCTTGATCGTTGCCACTCAGTTCCGGTTCCGCTACAACCATTTCAA
  AAACGATGATTCTAAAGGGAAAGAGGTGGAGATCCCGCAGGTGGAAAGGAACAACTCTACAGAGTCCTCT
  GAGGAGACACCAACCACCACAAGCCACTCGCAGGCCTTCAACTTTTGGGACTTAATCAAGCTGCTCTGCA
  GCGTGCAGTATGGCTCAGTGCTGTTTGTGGCTTGGTTCATGGGTTTTGGATATGGCTTCGTGTTCACCTT
  TCTCTACTGGCATTTGGAAGACCTCAATGGAACTACAACCCTCTTTGGGGTCTGTTCAGTCCTGAGTCAT
  GTGTCTGAGCTGACAGCATATTTTTTTAGTCACAAGCTTATTGAATTGATCGGCCACATCAGGGTTCTGT
  ACATTGGCCTGGCCTGCAATACGGCTCGCTATATTTATATTTCCTACCTGGAGAATGCCTGGACTGTTCT
  CCCCATGGAAGTTCTTCAAGGAGTGACACACGCGGCCATCTGGGCAGCATGCATTTCTTACCTCAGTGCA
  GCCGTTCCCCCTGAGCTGAGGACATCTGCTCAGGGCATCCTGCAGGGCCTTCACCTGGGTTTGGGAAGAG
  GATGTGGTGCCATGATCGGAGGCGTGTTAGTCAATTATTTTGGGGCTGCTGCAACCTTCCGAGGAATTGG
  CATGGCCTGCTTGGTGATCCTACTGCTCTTTGCCCTGATCCAGTGGCTGGCAGTGCCAGATGAGGAAGAA
  GACAAGACAATGTTGGCAGAAAGAATTCCTGTTCCCTCCAGTCCCGTTCCTATAGCAACCATCGACTTGG
  TACAGCAACAGACAGAAGATGTCATGCCACGCATTGAGCCCAGACTTCCACCCAAGAAAACTAAGCACCA
  GGAAGAACAGGAAGATGTGAACAAACCAGCCTGGGGAGTCAGCTCTTCTCCCTGGGTGACCTTTGTCTAT
  GCACTCTACCAAATTAAAGAGATGATGCAACTCACAAGAGACAACCGTGCTTCTGAGATACAGCCTTTAC
  AGGGGACCAATGAGAATAGGGAAAATTCTCCTGCTGGTAGAGCCCAGCCTGTCCCATGTGAGACTCACTC
  TGACCCATCTAGAAACCAGCCATCCCCTGACGCAGCAGCATCTCAGACGCAGACCAGCCCCGCTCACCCC
  AGTGTGGACCCGTGCACAGAGGAGAGTGAAGAGCAGCAGGCTCAGCTGGCCGCGGGAGGACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219252 representing NM_017694
  Red=Cloning site Green=Tags(s)
  
  MADDKVAILTDDEEEQKRKYVLADPFNGISREPEPPSNETPSSTETSAIPEEEIDWIEKHCVKINNDLLI
  SKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVVADRFKKGKIVLLFSLL
  CWVLFNLGIGFVKPATLRCVPKIRPTTHPTNASHQLTILPTNSSFTSFLTISPKMREKRNLLETGLNVSD
  TVTLPTAPNMNSEPTLQPQTGEITNRMMDLTLNSSTATPVSPGSVTKETTTVIVTTTKSLPSDQVMLVYD
  QQEVEAIFLVILVVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTH
  IEVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFKNDDSKGKEVEIPQVERNNSTESS
  EETPTTTSHSQAFNFWDLIKLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSH
  VSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSA
  AVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPDEEE
  DKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRIEPRLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVY
  ALYQIKEMMQLTRDNRASEIQPLQGTNENRENSPAGRAQPVPCETHSDPSRNQPSPDAAASQTQTSPAHP
  SVDPCTEESEEQQAQLAAGGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017694
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017694.2, NP_060164.2
• RefSeq Size: 4320 bp
• RefSeq ORF: 2376 bp
• Locus ID: 54842
• UniProt ID: Q6ZSS7
• Cytogenetics: 2q32.2
• Protein Families: Transmembrane
• MW: 87.9 kDa