SAMD9 (NM_017654) Human Tagged ORF Clone

CAT#: RC219076

SAMD9 (Myc-DDK-tagged)-Human sterile alpha motif domain containing 9 (SAMD9), transcript variant 1

Product Images

Circular map for RC219076

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SAMD9
• Synonyms: C7orf5; DRIF1; M7MLS2; MIRAGE; NFTC; OEF1; OEF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219076 representing NM_017654
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGCAACTTAACCTTCCAGAAAATACAGATGATTGGACAAAAGAGGATGTAAATCAGTGGTTAG
  AAAGTCATAAGATTGACCAAAAACACAGGGAAATTTTGACTGAACAAGACGTGAATGGAGCAGTCTTGAA
  GTGGTTAAAAAAAGAACATCTTGTTGATATGGGCATCACACATGGACCAGCTATTCAAATAGAAGAACTA
  TTCAAAGAATTGCGGAAAACAGCCATTGAAGATTCGATTCAGACATCTAAGATGGGAAAGCCCAGTAAAA
  ATGCTCCTAAAGACCAAACTGTGTCTCAAAAGGAACGTAGAGAAACTTCAAAGCAAAAACAAAAGGGTAA
  AGAGAACCCAGATATGGCTAATCCGTCTGCAATGAGTACAACTGCTAAAGGTTCTAAGTCACTAAAAGTT
  GAGCTCATAGAAGATAAAATAGATTATACAAAGGAAAGGCAACCATCCATAGACCTGACATGTGTATCAT
  ATCCATTTGATGAATTCAGTAATCCATATCGTTACAAGTTGGATTTTAGTCTACAGCCTGAAACAGGACC
  AGGCAATCTCATTGATCCGATACATGAATTCAAAGCCTTCACAAATACAGCAACAGCCACAGAAGAGGAT
  GTCAAGATGAAATTTAGCAATGAGGTTTTCCGATTTGCTTCAGCTTGTATGAATTCACGTACCAATGGCA
  CTATTCATTTTGGAGTCAAAGACAAACCCCATGGGAAAATTGTTGGCATCAAAGTCACCAATGATACCAA
  GGAAGCCCTCATTAACCATTTCAATCTGATGATAAACAAGTATTTTGAAGACCATCAAGTCCAACAAGCA
  AAGAAGTGCATTCGAGAGCCAAGATTTGTGGAAGTTTTACTGCCAAATAGTACTCTATCTGACAGATTTG
  TTATTGAAGTGGACATTATTCCACAGTTCTCTGAATGCCAATATGATTATTTCCAGATTAAAATGCAAAA
  TTACAACAACAAAATATGGGAACAAAGTAAAAAATTCTCACTATTTGTGCGAGATGGGACCAGCTCTAAG
  GACATTACGAAAAATAAAGTTGATTTCAGAGCATTTAAAGCAGATTTTAAAACACTGGCAGAGTCCAGAA
  AAGCAGCAGAAGAAAAATTCAGAGCAAAAACAAATAAAAAAGAAAGAGAGGGACCAAAGTTGGTTAAATT
  ATTGACAGGAAATCAAGATTTGTTAGATAATTCATACTATGAACAGTACATTCTTGTAACAAATAAATGC
  CACCCAGATCAAACAAAACACTTAGATTTCCTGAAGGAAATTAAATGGTTTGCTGTATTGGAGTTTGATC
  CTGAGTCTAACATCAATGGAGTGGTCAAAGCTTACAAAGAAAGCCGAGTAGCAAACCTTCACTTTCCAAG
  TGTATATGTAGAACAGAAAACCACACCAAATGAGACGATTTCTACTCTAAATCTTTACCATCAACCCAGC
  TGGATTTTCTGCAATGGCAGGTTAGACCTTGACAGTGAAAAATATAAACCCTTTGATCCAAGTTCCTGGC
  AAAGAGAAAGAGCTTCTGATGTCAGGAAACTGATTTCATTTCTTACACATGAAGACATAATGCCAAGAGG
  GAAGTTTTTGGTGGTATTTCTATTACTGTCCTCTGTGGATGACCCAAGAGATCCCCTCATTGAGACTTTC
  TGTGCTTTCTACCAGGATCTCAAAGGAATGGAAAATATACTGTGTATTTGTGTGCACCCACACATATTTC
  AGGGATGGAAAGATCTACTTGAAGCAAGATTAATAAAACACCAAGATGAAATTTCAAGCCAATGTATTTC
  TGCTTTAAGCCTTGAAGAGATCAATGGCACTATTCTTAAACTAAAATCTGTGACTCAATCTTCAAAAAGG
  CTTTTGCCATCTATTGGTTTATCGACTGTCCTTCTGAAAAAGGAAGAAGATATCATGACTGCTCTGGAAA
  TTATCTGTGAAAATGAATGTGAGGGTACACTGTTAGAGAAGGACAAAAATAAATTCCTTGAATTCAAGGC
  ATCAAAAGAGGAAGACTTCTATCGAGGTGGCAAAGTGTCATGGTGGAACTTCTACTTCTCTTCTGAAAGT
  TATTCTTCACCTTTTGTCAAAAGGGATAAATATGAAAGACTTGAAGCAATGATTCAAAACTGTGCAGATT
  CTTCTAAACCAACAAGTACCAAAATTATTCATCTGTATCATCATCCAGGCTGTGGGGGAACTACCTTGGC
  TATGCACATTCTCTGGGAACTAAGGAAGAAATTCAGATGTGCTGTGCTGAAAAACAAGACAGTGGATTTT
  TCTGAAATTGGAGAACAGGTAACCAGTTTAATCACCTATGGGGCAATGAACCGTCAGGAATACGTACCTG
  TACTACTCCTTGTTGATGATTTTGAAGAACAAGATAATGTCTATCTTCTGCAGTACTCTATTCAAACAGC
  TATAGCTAAAAAGTACATTCGATATGAAAAACCTCTGGTGATTATCCTAAATTGTATGAGATCACAAAAT
  CCTGAAAAAAGTGCAAGGATCCCAGACAGTATTGCCGTAATACAGCAACTCTCTCCCAAAGAACAGAGAG
  CTTTTGAGCTTAAATTGAAAGAAATCAAAGAACAGCATAAAAACTTTGAGGATTTTTATTCCTTTATGAT
  CATGAAAACCAATTTTAATAAAGAATACATAGAAAATGTGGTCCGGAATATCCTGAAAGGGCAGAATATT
  TTCACCAAGGAAGCAAAGCTCTTTTCTTTTCTGGCTCTTCTTAATTCATATGTGCCTGATACCACCATTT
  CACTATCACAGTGTGAAAAATTCTTAGGAATTGGAAACAAGAAGGCTTTCTGGGGGACAGAAAAATTTGA
  AGACAAGATGGGCACCTACTCTACAATTCTGATAAAAACAGAGGTCATCGAATGTGGGAACTACTGTGGA
  GTACGCATCATTCACTCTTTGATTGCAGAGTTCTCACTGGAAGAATTGAAGAAAAGCTATCACCTGAATA
  AAAGTCAAATTATGTTGGATATGCTAACTGAGAATTTGTTCTTCGATACTGGTATGGGAAAAAGTAAATT
  TTTGCAAGATATGCACACACTCCTACTCACAAGACACCGCGATGAACATGAAGGTGAAACAGGAAATTGG
  TTTTCCCCATTTATTGAAGCATTACATAAAGATGAAGGAAATGAAGCAGTTGAAGCTGTATTGCTTGAAA
  GTATCCATCGGTTCAACCCAAATGCATTCATTTGCCAAGCGTTGGCAAGACATTTCTACATTAAAAAGAA
  GGACTTTGGCAATGCTCTAAACTGGGCAAAACAAGCAAAAATCATAGAACCTGACAATTCTTATATCTCA
  GATACACTGGGTCAAGTCTACAAAAGTAAAATAAGATGGTGGATAGAGGAAAACGGAGGAAACGGGAACA
  TTTCAGTTGATGATCTAATTGCTCTTTTGGATTTAGCAGAACATGCCTCAAGTGCATTCAAAGAATCTCA
  ACAGCAAAGTGAAGATAGAGAGTATGAAGTGAAGGAAAGATTGTATCCGAAGTCAAAAAGGCGGTATGAT
  ACTTACAATATAGCTGGTTATCAAGGAGAGATAGAAGTTGGGCTTTACACAATCCAAATTCTCCAGCTCA
  TTCCTTTTTTTGATAATAAAAATGAGCTATCTAAAAGATATATGGTCAATTTTGTATCAGGAAGTAGTGA
  TATTCCAGGGGATCCAAACAATGAATATAAATTAGCCCTCAAAAACTATATTCCTTATTTAACTAAATTG
  AAATTTTCTTTGAAAAAGTCCTTTGATTTTTTTGATGAATACTTTGTCCTGCTAAAACCCAGGAACAATA
  TTAAGCAAAATGAAGAGGCCAAAACTCGGAGAAAGGTGGCTGGATATTTTAAGAAATATGTAGATATATT
  TTGTCTCTTAGAAGAATCACAAAACAACACAGGTCTTGGATCAAAGTTCAGTGAGCCACTTCAAGTAGAG
  AGATGCAGGAGAAACCTAGTAGCTTTAAAAGCAGACAAGTTTTCTGGGCTCTTGGAATATCTTATCAAAA
  GTCAAGAGGATGCTATAAGCACTATGAAATGTATAGTGAACGAATATACTTTTCTCTTAGAACAATGCAC
  TGTCAAAATCCAGTCAAAAGAAAAGCTAAATTTCATCTTGGCCAACATTATTCTCTCCTGTATCCAACCT
  ACCTCCAGATTAGTAAAGCCAGTTGAAAAACTAAAAGATCAGCTTCGAGAAGTCTTGCAACCAATAGGAC
  TGACTTATCAGTTTTCAGAACCGTATTTTCTAGCTTCCCTCTTATTCTGGCCAGAAAATCAACAACTAGA
  TCAACATTCTGAACAAATGAAAGAGTATGCTCAAGCACTAAAAAATTCTTTCAAGGGGCAATATAAACAT
  ATGCATCGTACAAAGCAACCAATTGCATATTTCTTTCTTGGAAAAGGTAAAAGACTGGAAAGACTTGTTC
  ACAAAGGAAAAATTGACCAGTGCTTTAAGAAGACACCAGATATTAATTCCTTGTGGCAGAGTGGAGATGT
  GTGGAAGGAGGAAAAAGTCCAAGAACTTTTGCTTCGTTTACAAGGTCGAGCTGAAAACAATTGTTTATAT
  ATAGAATATGGAATCAATGAAAAAATCACAATACCCATCACTCCCGCTTTTTTAGGTCAACTTAGAAGTG
  GCAGAAGCATAGAGAAGGTGTCTTTTTACCTGGGATTTTCCATTGGAGGCCCACTTGCTTATGACATTGA
  AATTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219076 representing NM_017654
  Red=Cloning site Green=Tags(s)
  
  MAKQLNLPENTDDWTKEDVNQWLESHKIDQKHREILTEQDVNGAVLKWLKKEHLVDMGITHGPAIQIEEL
  FKELRKTAIEDSIQTSKMGKPSKNAPKDQTVSQKERRETSKQKQKGKENPDMANPSAMSTTAKGSKSLKV
  ELIEDKIDYTKERQPSIDLTCVSYPFDEFSNPYRYKLDFSLQPETGPGNLIDPIHEFKAFTNTATATEED
  VKMKFSNEVFRFASACMNSRTNGTIHFGVKDKPHGKIVGIKVTNDTKEALINHFNLMINKYFEDHQVQQA
  KKCIREPRFVEVLLPNSTLSDRFVIEVDIIPQFSECQYDYFQIKMQNYNNKIWEQSKKFSLFVRDGTSSK
  DITKNKVDFRAFKADFKTLAESRKAAEEKFRAKTNKKEREGPKLVKLLTGNQDLLDNSYYEQYILVTNKC
  HPDQTKHLDFLKEIKWFAVLEFDPESNINGVVKAYKESRVANLHFPSVYVEQKTTPNETISTLNLYHQPS
  WIFCNGRLDLDSEKYKPFDPSSWQRERASDVRKLISFLTHEDIMPRGKFLVVFLLLSSVDDPRDPLIETF
  CAFYQDLKGMENILCICVHPHIFQGWKDLLEARLIKHQDEISSQCISALSLEEINGTILKLKSVTQSSKR
  LLPSIGLSTVLLKKEEDIMTALEIICENECEGTLLEKDKNKFLEFKASKEEDFYRGGKVSWWNFYFSSES
  YSSPFVKRDKYERLEAMIQNCADSSKPTSTKIIHLYHHPGCGGTTLAMHILWELRKKFRCAVLKNKTVDF
  SEIGEQVTSLITYGAMNRQEYVPVLLLVDDFEEQDNVYLLQYSIQTAIAKKYIRYEKPLVIILNCMRSQN
  PEKSARIPDSIAVIQQLSPKEQRAFELKLKEIKEQHKNFEDFYSFMIMKTNFNKEYIENVVRNILKGQNI
  FTKEAKLFSFLALLNSYVPDTTISLSQCEKFLGIGNKKAFWGTEKFEDKMGTYSTILIKTEVIECGNYCG
  VRIIHSLIAEFSLEELKKSYHLNKSQIMLDMLTENLFFDTGMGKSKFLQDMHTLLLTRHRDEHEGETGNW
  FSPFIEALHKDEGNEAVEAVLLESIHRFNPNAFICQALARHFYIKKKDFGNALNWAKQAKIIEPDNSYIS
  DTLGQVYKSKIRWWIEENGGNGNISVDDLIALLDLAEHASSAFKESQQQSEDREYEVKERLYPKSKRRYD
  TYNIAGYQGEIEVGLYTIQILQLIPFFDNKNELSKRYMVNFVSGSSDIPGDPNNEYKLALKNYIPYLTKL
  KFSLKKSFDFFDEYFVLLKPRNNIKQNEEAKTRRKVAGYFKKYVDIFCLLEESQNNTGLGSKFSEPLQVE
  RCRRNLVALKADKFSGLLEYLIKSQEDAISTMKCIVNEYTFLLEQCTVKIQSKEKLNFILANIILSCIQP
  TSRLVKPVEKLKDQLREVLQPIGLTYQFSEPYFLASLLFWPENQQLDQHSEQMKEYAQALKNSFKGQYKH
  MHRTKQPIAYFFLGKGKRLERLVHKGKIDQCFKKTPDINSLWQSGDVWKEEKVQELLLRLQGRAENNCLY
  IEYGINEKITIPITPAFLGQLRSGRSIEKVSFYLGFSIGGPLAYDIEIV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017654
• ORF Size: 4767 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017654.4
• RefSeq Size: 6855 bp
• RefSeq ORF: 4770 bp
• Locus ID: 54809
• UniProt ID: Q5K651
• Cytogenetics: 7q21.2
• MW: 184.3 kDa
• Gene Summary: This gene encodes a sterile alpha motif domain-containing protein. The encoded protein localizes to the cytoplasm and may play a role in regulating cell proliferation and apoptosis. Mutations in this gene are the cause of normophosphatemic familial tumoral calcinosis. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Jul 2010]