ARSK (NM_198150) Human Tagged ORF Clone

CAT#: RC218648

ARSK (Myc-DDK-tagged)-Human arylsulfatase family, member K (ARSK)

Product Images

Circular map for RC218648

• 

  Western blot validation of overexpression lysate (Cat# LY405061) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218648 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARSK
• Synonyms: TSULF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218648 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACTGCTGTGGGTGTCGGTGGTCGCAGCCTTGGCGCTGGCGGTACTGGCCCCCGGAGCAGGGGAGC
  AGAGGCGGAGAGCAGCCAAAGCGCCCAATGTGGTGCTGGTCGTGAGCGACTCCTTCGATGGAAGGTTAAC
  ATTTCATCCAGGAAGTCAGGTAGTGAAACTTCCTTTTATCAACTTTATGAAGACACGTGGGACTTCCTTT
  CTGAATGCCTACACAAACTCTCCAATTTGTTGCCCATCACGCGCAGCAATGTGGAGTGGCCTCTTCACTC
  ACTTAACAGAATCTTGGAATAATTTTAAGGGTCTAGATCCAAATTATACAACATGGATGGATGTCATGGA
  GAGGCATGGCTACCGAACACAGAAATTTGGGAAACTGGACTATACTTCAGGACATCACTCCATTAGTAAT
  CGTGTGGAAGCGTGGACAAGAGATGTTGCTTTCTTACTCAGACAAGAAGGCAGGCCCATGGTTAATCTTA
  TCCGTAACAGGACTAAAGTCAGAGTGATGGAAAGGGATTGGCAGAATACAGACAAAGCAGTAAACTGGTT
  AAGAAAGGAAGCAATTAATTACACTGAACCATTTGTTATTTACTTGGGATTAAATTTACCACACCCTTAC
  CCTTCACCATCTTCTGGAGAAAATTTTGGATCTTCAACATTTCACACATCTCTTTATTGGCTTGAAAAAG
  TGTCTCATGATGCCATCAAAATCCCAAAGTGGTCACCTTTGTCAGAAATGCACCCTGTAGATTATTACTC
  TTCTTATACAAAAAACTGCACTGGAAGATTTACAAAAAAAGAAATTAAGAATATTAGAGCATTTTATTAT
  GCTATGTGTGCTGAGACAGATGCCATGCTTGGTGAAATTATTTTGGCCCTTCATCAATTAGATCTTCTTC
  AGAAAACTATTGTCATATACTCCTCAGACCATGGAGAGCTGGCCATGGAACATCGACAGTTTTATAAAAT
  GAGCATGTACGAGGCTAGTGCACATGTTCCGCTTTTGATGATGGGACCAGGAATTAAAGCCGGCCTACAA
  GTATCAAATGTGGTTTCTCTTGTGGATATTTACCCTACCATGCTTGATATTGCTGGAATTCCTCTGCCTC
  AGAACCTGAGTGGATACTCTTTGTTGCCGTTATCATCAGAAACATTTAAGAATGAACATAAAGTCAAAAA
  CCTGCATCCACCCTGGATTCTGAGTGAATTCCATGGATGTAATGTGAATGCCTCCACCTACATGCTTCGA
  ACTAACCACTGGAAATATATAGCCTATTCGGATGGTGCATCAATATTGCCTCAACTCTTTGATCTTTCCT
  CGGATCCAGATGAATTAACAAATGTTGCTGTAAAATTTCCAGAAATTACTTATTCTTTGGATCAGAAGCT
  TCATTCCATTATAAACTACCCTAAAGTTTCTGCTTCTGTCCACCAGTATAATAAAGAGCAGTTTATCAAG
  TGGAAACAAAGTATAGGACAGAATTATTCAAACGTTATAGCAAATCTTAGGTGGCACCAAGACTGGCAGA
  AGGAACCAAGGAAGTATGAAAATGCAATTGATCAGTGGCTTAAAACCCATATGAATCCAAGAGCAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218648 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLLWVSVVAALALAVLAPGAGEQRRRAAKAPNVVLVVSDSFDGRLTFHPGSQVVKLPFINFMKTRGTSF
  LNAYTNSPICCPSRAAMWSGLFTHLTESWNNFKGLDPNYTTWMDVMERHGYRTQKFGKLDYTSGHHSISN
  RVEAWTRDVAFLLRQEGRPMVNLIRNRTKVRVMERDWQNTDKAVNWLRKEAINYTEPFVIYLGLNLPHPY
  PSPSSGENFGSSTFHTSLYWLEKVSHDAIKIPKWSPLSEMHPVDYYSSYTKNCTGRFTKKEIKNIRAFYY
  AMCAETDAMLGEIILALHQLDLLQKTIVIYSSDHGELAMEHRQFYKMSMYEASAHVPLLMMGPGIKAGLQ
  VSNVVSLVDIYPTMLDIAGIPLPQNLSGYSLLPLSSETFKNEHKVKNLHPPWILSEFHGCNVNASTYMLR
  TNHWKYIAYSDGASILPQLFDLSSDPDELTNVAVKFPEITYSLDQKLHSIINYPKVSASVHQYNKEQFIK
  WKQSIGQNYSNVIANLRWHQDWQKEPRKYENAIDQWLKTHMNPRAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198150
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198150.3
• RefSeq Size: 3345 bp
• RefSeq ORF: 1611 bp
• Locus ID: 153642
• UniProt ID: Q6UWY0
• Cytogenetics: 5q15
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 61.5 kDa
• Gene Summary: Sulfatases (EC 3.1.5.6), such as ARSK, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules (Sardiello et al., 2005 [PubMed 16174644]).[supplied by OMIM, Mar 2008]