ANTXR2 (NM_058172) Human Tagged ORF Clone

CAT#: RC218589

ANTXR2 (Myc-DDK-tagged)-Human anthrax toxin receptor 2 (ANTXR2), transcript variant 1

Product Images

Circular map for RC218589

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  Western blot validation of overexpression lysate (Cat# LY403300) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218589 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ANTXR2 protein (Cat# TP318589). The protein was produced from HEK293T cells transfected with ANTXR2 cDNA clone (Cat# RC218589) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANTXR2
• Synonyms: CMG-2; CMG2; HFS; ISH; JHF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218589 representing NM_058172
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCGGAGCGGTCCCCGGCCCGCAGCCCCGGGAGCTGGCTGTTCCCCGGGCTGTGGCTGTTGGTGC
  TCAGCGGTCCCGGGGGGCTGCTGCGCGCCCAGGAGCAGCCCTCCTGCAGAAGAGCCTTTGATCTCTACTT
  CGTCCTGGACAAGTCTGGGAGTGTGGCAAATAACTGGATTGAAATTTATAATTTCGTACAGCAACTTGCG
  GAGAGATTTGTGAGCCCTGAAATGAGATTATCTTTCATTGTGTTTTCTTCTCAAGCAACTATTATTTTGC
  CATTAACTGGAGACAGAGGCAAAATCAGTAAAGGCTTGGAGGATTTAAAACGTGTTAGTCCAGTAGGAGA
  GACATATATCCATGAAGGACTAAAGCTAGCGAATGAACAAATTCAGAAAGCAGGAGGCTTGAAAACCTCC
  AGTATCATAATTGCTCTGACAGATGGCAAGTTGGACGGTCTGGTGCCATCATATGCAGAGAAAGAGGCAA
  AGATATCCAGGTCACTTGGGGCTAGTGTTTATTGTGTTGGTGTCCTTGATTTTGAACAAGCACAGCTTGA
  AAGAATTGCTGATTCCAAGGAGCAAGTTTTCCCTGTCAAAGGTGGATTTCAGGCTCTTAAAGGAATAATT
  AATTCTATACTAGCTCAGTCATGTACTGAAATCCTAGAATTGCAGCCCTCAAGTGTCTGTGTGGGGGAGG
  AATTTCAGATTGTCTTAAGTGGAAGAGGATTCATGCTGGGCAGTCGGAATGGCAGTGTTCTCTGCACTTA
  CACTGTAAATGAAACATATACAACGAGTGTAAAACCAGTAAGTGTACAGCTTAATTCTATGCTTTGTCCT
  GCACCTATCCTGAATAAAGCTGGAGAAACTCTTGATGTTTCAGTGAGCTTTAATGGAGGAAAATCTGTCA
  TTTCAGGATCATTAATTGTCACAGCCACAGAATGTTCTAACGGGATCGCAGCCATCATTGTTATTTTGGT
  GTTACTGCTACTCCTGGGGATCGGTTTGATGTGGTGGTTTTGGCCCCTTTGCTGCAAAGTGGTTATTAAG
  GATCCTCCACCACCACCCCCCCCTGCACCAAAAGAGGAGGAAGAAGAACCTTTGCCTACTAAAAAGTGGC
  CAACTGTGGATGCTTCCTATTATGGTGGTCGAGGGGTTGGAGGAATTAAAAGAATGGAGGTTCGTTGGGG
  TGATAAAGGATCTACTGAGGAAGGTGCAAGGCTAGAGAAAGCCAAAAATGCTGTGGTGAAGATTCCTGAA
  GAAACAGAGGAACCCATCAGGCCTAGACCACCTCGACCCAAACCCACACACCAGCCTCCTCAGACAAAAT
  GGTACACCCCAATTAAGGGTCGTCTTGATGCTCTCTGGGCTTTGTTGAGGCGGCAGTATGACCGGGTTTC
  TTTGATGCGACCTCAGGAAGGAGATGAGGGCCGGTGCATAAACTTCTCCCGAGTTCCATCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218589 representing NM_058172
  Red=Cloning site Green=Tags(s)
  
  MVAERSPARSPGSWLFPGLWLLVLSGPGGLLRAQEQPSCRRAFDLYFVLDKSGSVANNWIEIYNFVQQLA
  ERFVSPEMRLSFIVFSSQATIILPLTGDRGKISKGLEDLKRVSPVGETYIHEGLKLANEQIQKAGGLKTS
  SIIIALTDGKLDGLVPSYAEKEAKISRSLGASVYCVGVLDFEQAQLERIADSKEQVFPVKGGFQALKGII
  NSILAQSCTEILELQPSSVCVGEEFQIVLSGRGFMLGSRNGSVLCTYTVNETYTTSVKPVSVQLNSMLCP
  APILNKAGETLDVSVSFNGGKSVISGSLIVTATECSNGIAAIIVILVLLLLLGIGLMWWFWPLCCKVVIK
  DPPPPPPPAPKEEEEEPLPTKKWPTVDASYYGGRGVGGIKRMEVRWGDKGSTEEGARLEKAKNAVVKIPE
  ETEEPIRPRPPRPKPTHQPPQTKWYTPIKGRLDALWALLRRQYDRVSLMRPQEGDEGRCINFSRVPSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_058172
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058172.6
• RefSeq Size: 3881 bp
• RefSeq ORF: 1467 bp
• Locus ID: 118429
• UniProt ID: P58335
• Cytogenetics: 4q21.21
• Protein Families: Druggable Genome, Transmembrane
• MW: 53.3 kDa
• Gene Summary: This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]