HDAC10 (NM_032019) Human Tagged ORF Clone

CAT#: RC218536

HDAC10 (Myc-DDK-tagged)-Human histone deacetylase 10 (HDAC10), transcript variant 1

Product Images

Circular map for RC218536

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HDAC10 (Cat# RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10(Cat# TA500751). Positive lysates LY403142 (100ug) and LC403142 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403142) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218536 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HDAC10 protein (Cat# TP318536). The protein was produced from HEK293T cells transfected with HDAC10 cDNA clone (Cat# RC218536) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HDAC10
• Synonyms: HD10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218536 representing NM_032019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCGCGCTTGTGTACCATGAGGACATGACGGCCACCCGGCTGCTCTGGGACGACCCCGAGTGCG
  AGATCGAGCGTCCTGAGCGCCTGACCGCAGCCCTGGATCGCCTGCGGCAGCGCGGCCTGGAACAGAGGTG
  TCTGCGGTTGTCAGCCCGCGAGGCCTCGGAAGAGGAGCTGGGCCTGGTGCACAGCCCAGAGTATGTATCC
  CTGGTCAGGGAGACCCAGGTCCTAGGCAAGGAGGAGCTGCAGGCGCTGTCCGGACAGTTCGACGCCATCT
  ACTTCCACCCGAGTACCTTTCACTGCGCGCGGCTGGCCGCAGGGGCTGGACTGCAGCTGGTGGACGCTGT
  GCTCACTGGAGCTGTGCAAAATGGGCTTGCCCTGGTGAGGCCTCCCGGGCACCATGGCCAGAGGGCGGCT
  GCCAACGGGTTCTGTGTGTTCAACAACGTGGCCATAGCAGCTGCACATGCCAAGCAGAAACACGGGCTAC
  ACAGGATCCTCGTCGTGGACTGGGATGTGCACCATGGCCAGGGGATCCAGTATCTCTTTGAGGATGACCC
  CAGCGTCCTTTACTTCTCCTGGCACCGCTATGAGCATGGGCGCTTCTGGCCTTTCCTGCGAGAGTCAGAT
  GCAGACGCAGTGGGGCGGGGACAGGGCCTCGGCTTCACTGTCAACCTGCCCTGGAACCAGGTTGGGATGG
  GAAACGCTGACTACGTGGCTGCCTTCCTGCACCTGCTGCTCCCACTGGCCTTTGAGTTTGACCCTGAGCT
  GGTGCTGGTCTCGGCAGGATTTGACTCAGCCATCGGGGACCCTGAGGGGCAAATGCAGGCCACGCCAGAG
  TGCTTCGCCCACCTCACACAGCTGCTGCAGGTGCTGGCCGGCGGCCGGGTCTGTGCCGTGCTGGAGGGCG
  GCTACCACCTGGAGTCACTGGCGGAGTCAGTGTGCATGACAGTACAGACGCTGCTGGGTGACCCGGCCCC
  ACCCCTGTCAGGGCCAATGGCGCCATGTCAGAGTGCCCTAGAGTCCATCCAGAGTGCCCGTGCTGCCCAG
  GCCCCGCACTGGAAGAGCCTCCAGCAGCAAGATGTGACCGCTGTGCCGATGAGCCCCAGCAGCCACTCCC
  CAGAGGGGAGGCCTCCACCTCTGCTGCCTGGGGGTCCAGTGTGTAAGGCAGCTGCATCTGCACCGAGCTC
  CCTCCTGGACCAGCCGTGCCTCTGCCCCGCACCCTCTGTCCGCACCGCTGTTGCCCTGACAACGCCGGAT
  ATCACATTGGTTCTGCCCCCTGACGTCATCCAACAGGAAGCGTCAGCCCTGAGGGAGGAGACAGAAGCCT
  GGGCCAGGCCACACGAGTCCCTGGCCCGGGAGGAGGCCCTCACTGCACTTGGGAAGCTCCTGTACCTCTT
  AGATGGGATGCTGGATGGGCAGGTGAACAGTGGTATAGCAGCCACTCCAGCCTCTGCTGCAGCAGCCACC
  CTGGATGTGGCTGTTCGGAGAGGCCTGTCCCACGGAGCCCAGAGGCTGCTGTGCGTGGCCCTGGGACAGC
  TGGACCGGCCTCCAGACCTCGCCCATGACGGGAGGAGTCTGTGGCTGAACATCAGGGGCAAGGAGGCGGC
  TGCCCTATCCATGTTCCATGTCTCCACGCCACTGCCAGTGATGACCGGTGGTTTCCTGAGCTGCATCTTG
  GGCTTGGTGCTGCCCCTGGCCTATGGCTTCCAGCCTGACCTGGTGCTGGTGGCGCTGGGGCCTGGCCATG
  GCCTGCAGGGCCCCCACGCTGCACTCCTGGCTGCAATGCTTCGGGGGCTGGCAGGGGGCCGAGTCCTGGC
  CCTCCTGGAGGAGAACTCCACACCCCAGCTAGCAGGGATCCTGGCCCGGGTGCTGAATGGAGAGGCACCT
  CCTAGCCTAGGCCCTTCCTCTGTGGCCTCCCCAGAGGACGTCCAGGCCCTGATGTACCTGAGAGGGCAGC
  TGGAGCCTCAGTGGAAGATGTTGCAGTGCCATCCTCACCTGGTGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218536 representing NM_032019
  Red=Cloning site Green=Tags(s)
  
  MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVS
  LVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAA
  ANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESD
  ADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPE
  CFAHLTQLLQVLAGGRVCAVLEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQ
  APHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPD
  ITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAAT
  LDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCIL
  GLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAP
  PSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032019
• ORF Size: 2007 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032019.6
• RefSeq Size: 2702 bp
• RefSeq ORF: 2010 bp
• Locus ID: 83933
• UniProt ID: Q969S8
• Cytogenetics: 22q13.33
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Transcription Factors
• MW: 71.3 kDa
• Gene Summary: The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]