HDAC10 (NM_032019) Human Tagged ORF Clone

CAT#: RC218536

Reviews ()
Write a review

HDAC10 (Myc-DDK-tagged)-Human histone deacetylase 10 (HDAC10), transcript variant 1



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HDAC10
Synonyms HD10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218536 representing NM_032019
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACCGCGCTTGTGTACCATGAGGACATGACGGCCACCCGGCTGCTCTGGGACGACCCCGAGTGCG
AGATCGAGCGTCCTGAGCGCCTGACCGCAGCCCTGGATCGCCTGCGGCAGCGCGGCCTGGAACAGAGGTG
TCTGCGGTTGTCAGCCCGCGAGGCCTCGGAAGAGGAGCTGGGCCTGGTGCACAGCCCAGAGTATGTATCC
CTGGTCAGGGAGACCCAGGTCCTAGGCAAGGAGGAGCTGCAGGCGCTGTCCGGACAGTTCGACGCCATCT
ACTTCCACCCGAGTACCTTTCACTGCGCGCGGCTGGCCGCAGGGGCTGGACTGCAGCTGGTGGACGCTGT
GCTCACTGGAGCTGTGCAAAATGGGCTTGCCCTGGTGAGGCCTCCCGGGCACCATGGCCAGAGGGCGGCT
GCCAACGGGTTCTGTGTGTTCAACAACGTGGCCATAGCAGCTGCACATGCCAAGCAGAAACACGGGCTAC
ACAGGATCCTCGTCGTGGACTGGGATGTGCACCATGGCCAGGGGATCCAGTATCTCTTTGAGGATGACCC
CAGCGTCCTTTACTTCTCCTGGCACCGCTATGAGCATGGGCGCTTCTGGCCTTTCCTGCGAGAGTCAGAT
GCAGACGCAGTGGGGCGGGGACAGGGCCTCGGCTTCACTGTCAACCTGCCCTGGAACCAGGTTGGGATGG
GAAACGCTGACTACGTGGCTGCCTTCCTGCACCTGCTGCTCCCACTGGCCTTTGAGTTTGACCCTGAGCT
GGTGCTGGTCTCGGCAGGATTTGACTCAGCCATCGGGGACCCTGAGGGGCAAATGCAGGCCACGCCAGAG
TGCTTCGCCCACCTCACACAGCTGCTGCAGGTGCTGGCCGGCGGCCGGGTCTGTGCCGTGCTGGAGGGCG
GCTACCACCTGGAGTCACTGGCGGAGTCAGTGTGCATGACAGTACAGACGCTGCTGGGTGACCCGGCCCC
ACCCCTGTCAGGGCCAATGGCGCCATGTCAGAGTGCCCTAGAGTCCATCCAGAGTGCCCGTGCTGCCCAG
GCCCCGCACTGGAAGAGCCTCCAGCAGCAAGATGTGACCGCTGTGCCGATGAGCCCCAGCAGCCACTCCC
CAGAGGGGAGGCCTCCACCTCTGCTGCCTGGGGGTCCAGTGTGTAAGGCAGCTGCATCTGCACCGAGCTC
CCTCCTGGACCAGCCGTGCCTCTGCCCCGCACCCTCTGTCCGCACCGCTGTTGCCCTGACAACGCCGGAT
ATCACATTGGTTCTGCCCCCTGACGTCATCCAACAGGAAGCGTCAGCCCTGAGGGAGGAGACAGAAGCCT
GGGCCAGGCCACACGAGTCCCTGGCCCGGGAGGAGGCCCTCACTGCACTTGGGAAGCTCCTGTACCTCTT
AGATGGGATGCTGGATGGGCAGGTGAACAGTGGTATAGCAGCCACTCCAGCCTCTGCTGCAGCAGCCACC
CTGGATGTGGCTGTTCGGAGAGGCCTGTCCCACGGAGCCCAGAGGCTGCTGTGCGTGGCCCTGGGACAGC
TGGACCGGCCTCCAGACCTCGCCCATGACGGGAGGAGTCTGTGGCTGAACATCAGGGGCAAGGAGGCGGC
TGCCCTATCCATGTTCCATGTCTCCACGCCACTGCCAGTGATGACCGGTGGTTTCCTGAGCTGCATCTTG
GGCTTGGTGCTGCCCCTGGCCTATGGCTTCCAGCCTGACCTGGTGCTGGTGGCGCTGGGGCCTGGCCATG
GCCTGCAGGGCCCCCACGCTGCACTCCTGGCTGCAATGCTTCGGGGGCTGGCAGGGGGCCGAGTCCTGGC
CCTCCTGGAGGAGAACTCCACACCCCAGCTAGCAGGGATCCTGGCCCGGGTGCTGAATGGAGAGGCACCT
CCTAGCCTAGGCCCTTCCTCTGTGGCCTCCCCAGAGGACGTCCAGGCCCTGATGTACCTGAGAGGGCAGC
TGGAGCCTCAGTGGAAGATGTTGCAGTGCCATCCTCACCTGGTGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218536 representing NM_032019
Red=Cloning site Green=Tags(s)

MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVS
LVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAA
ANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESD
ADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPE
CFAHLTQLLQVLAGGRVCAVLEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQ
APHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPD
ITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAAT
LDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCIL
GLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAP
PSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032019
ORF Size 2007 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032019.6
RefSeq Size 2702 bp
RefSeq ORF 2010 bp
Locus ID 83933
UniProt ID Q969S8
Cytogenetics 22q13.33
Domains Hist_deacetyl
Protein Families Druggable Genome, Transcription Factors
MW 71.3 kDa
Gene Summary The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Other products for "HDAC10"
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.