DEFB130A (NM_001037804) Human Tagged ORF Clone
CAT#: RC218446
DEFB130 (Myc-DDK-tagged)-Human defensin, beta 130 (DEFB130)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001037804" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DEFB130A |
Synonyms | DEFB-30; DEFB30; DEFB130; DEFB130L |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218446 representing NM_001037804
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTCCATTCCCTTATTTCTGTTCTCCTCCTCTTTGTGACTTTAATACCAAAAGGAAAGACTGGCG TTATTCCAGGACAAAAACAGTGTATTGCTTTGAAAGGGGTGTGCAGAGACAAACTATGCAGCACACTAGA TGATACCATTGGTATATGTAATGAAGGAAAAAAATGTTGTAGAAGGTGGTGGATACTTGAGCCCTATCCA ACTCCGGTTCCCAAAGGAAAATCTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218446 representing NM_001037804
Red=Cloning site Green=Tags(s) MKLHSLISVLLLFVTLIPKGKTGVIPGQKQCIALKGVCRDKLCSTLDDTIGICNEGKKCCRRWWILEPYP TPVPKGKSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001037804 |
ORF Size | 237 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001037804.1, NP_001032893.1 |
RefSeq Size | 240 bp |
RefSeq ORF | 240 bp |
Locus ID | 245940 |
UniProt ID | P0DP74 |
Cytogenetics | 8p23.1 |
Protein Families | Secreted Protein |
MW | 8.6 kDa |
Gene Summary | Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 8p23. [provided by RefSeq, Nov 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218446L3 | Lenti ORF clone of Human defensin, beta 130 (DEFB130), Myc-DDK-tagged |
USD 450.00 |
|
RC218446L4 | Lenti ORF clone of Human defensin, beta 130 (DEFB130), mGFP tagged |
USD 450.00 |
|
RG218446 | DEFB130 (tGFP-tagged) - Human defensin, beta 130 (DEFB130) |
USD 350.00 |
|
SC302936 | DEFB130 (untagged)-Human defensin, beta 130 (DEFB130) |
USD 165.00 |
{0} Product Review(s)
Be the first one to submit a review