SMARCA1 (NM_003069) Human Tagged ORF Clone

CAT#: RC218308

SMARCA1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 (SMARCA1), transcript variant 1

Product Images

Circular map for RC218308

• 

  Coomassie blue staining of purified SMARCA1 protein (Cat# TP318308). The protein was produced from HEK293T cells transfected with SMARCA1 cDNA clone (Cat# RC218308) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMARCA1
• Synonyms: hSNF2L; ISWI; NURF140; SNF2L; SNF2L1; SNF2LB; SNF2LT; SWI; SWI2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218308 representing NM_003069
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGACACTGCCGCAGTGGCAGCCACCGTGGCAGCCGCGGATGCGACCGCCACTATCGTGGTCA
  TAGAGGACGAGCAGCCCGGGCCGTCCACCTCTCAGGAGGAGGGAGCGGCCGCCGCGGCCACCGAAGCCAC
  CGCGGCCACGGAGAAGGGCGAGAAGAAGAAGGAGAAAAACGTTTCTTCATTTCAACTCAAACTTGCTGCT
  AAAGCGCCTAAATCTGAAAAGGAAATGGACCCAGAATATGAAGAGAAAATGAAAGCCGACCGAGCAAAGA
  GATTTGAATTTTTACTGAAGCAGACAGAACTTTTTGCACATTTCATTCAGCCTTCAGCACAGAAATCTCC
  AACATCTCCACTGAACATGAAATTGGGACGTCCCCGAATAAAGAAAGATGAAAAGCAGAGCTTAATTTCT
  GCTGGAGACTACCGCCATAGGCGCACAGAGCAAGAAGAAGATGAAGAGCTACTGTCTGAGAGTCGGAAAA
  CATCTAATGTGTGTATTAGATTTGAGGTGTCACCTTCATATGTGAAAGGGGGGCCACTGAGAGATTATCA
  GATTCGAGGACTGAATTGGTTGATCTCTTTATATGAAAATGGAGTCAATGGCATTTTGGCTGATGAAATG
  GGCCTTGGGAAAACTTTACAAACAATTGCTTTGCTTGGTTACCTGAAACACTACCGAAATATTCCTGGAC
  CTCACATGGTTTTAGTTCCAAAGTCTACTTTACACAACTGGATGAATGAATTTAAACGATGGGTCCCATC
  TCTCCGTGTCATTTGTTTTGTCGGAGACAAGGATGCCAGAGCTGCTTTTATTCGTGATGAAATGATGCCA
  GGAGAGTGGGATGTTTGCGTTACTTCTTATGAGATGGTAATTAAAGAAAAATCTGTATTCAAAAAGTTTC
  ACTGGCGATACCTGGTCATTGATGAAGCTCACAGAATAAAGAATGAAAAATCTAAGCTTTCAGAGATTGT
  TCGTGAGTTCAAGTCGACTAACCGCTTGCTCCTAACTGGAACACCTTTGCAGAATAACCTGCATGAACTG
  TGGGCCTTACTCAACTTTTTATTGCCTGATGTCTTTAATTCTGCAGATGACTTTGATTCTTGGTTTGACA
  CTAAAAATTGTCTTGGTGATCAAAAACTCGTGGAAAGACTTCATGCAGTTTTAAAACCATTTTTGTTACG
  CCGTATAAAAACTGATGTAGAGAAGAGTCTGCCACCTAAAAAGGAAATAAAGATTTACTTGGGGCTGAGT
  AAGATGCAACGAGAATGGTATACAAAAATCCTGATGAAAGATATTGATGTTTTAAACTCTTCTGGCAAGA
  TGGACAAGATGCGACTCTTAAACATTCTGATGCAGCTTCGAAAGTGTTGTAATCATCCATATCTGTTTGA
  TGGTGCTGAACCTGGTCCACCTTATACCACTGATGAGCATATTGTCAGCAACAGTGGTAAAATGGTAGTT
  CTGGATAAACTATTGGCCAAACTCAAAGAACAGGGTTCAAGGGTTCTCATTTTCAGCCAGATGACTCGCT
  TGCTGGATATTTTGGAAGATTATTGCATGTGGCGTGGTTATGAGTATTGTCGACTGGATGGACAAACCCC
  GCATGAAGAAAGAGAGGATAAATTCCTAGAAGTGGAATTTCTGGGTCAAAGGGAAGCAATAGAGGCTTTT
  AATGCTCCTAATAGTAGCAAATTCATCTTTATGCTAAGTACCAGGGCTGGAGGTCTCGGAATTAACCTGG
  CAAGTGCTGATGTGGTTATACTATATGATTCAGACTGGAACCCACAGGTTGATCTACAAGCTATGGATCG
  AGCACATCGTATTGGTCAGAAGAAACCAGTACGTGTATTCCGTCTCATCACTGACAACACTGTTGAAGAG
  AGGATTGTAGAAAGAGCTGAGATAAAACTGAGACTCGATTCAATTGTTATACAACAAGGAAGACTCATTG
  ACCAACAGTCTAACAAGCTGGCAAAAGAGGAAATGTTACAAATGATACGGCATGGAGCCACCCATGTTTT
  TGCTTCTAAAGAGAGTGAGTTGACAGATGAAGACATTACAACTATTCTGGAAAGAGGGGAAAAGAAGACT
  GCAGAGATGAATGAACGCCTGCAAAAAATGGGAGAGTCTTCTCTAAGAAATTTTAGAATGGACATTGAAC
  AAAGTTTATACAAATTTGAGGGAGAAGATTATAGAGAAAAACAGAAGCTTGGCATGGTGGAATGGATTGA
  ACCTCCTAAACGAGAACGCAAAGCAAACTACGCAGTGGATGCCTACTTTAGAGAGGCTTTGCGTGTCAGC
  GAGCCAAAGATTCCAAAGGCTCCACGGCCTCCAAAACAGCCAAATGTTCAGGATTTTCAATTTTTCCCAC
  CACGCTTATTTGAGCTCCTGGAAAAGGAAATTCTTTATTATCGGAAGACAATAGGCTATAAGGTTCCAAG
  GAATCCTGATATCCCAAATCCAGCTCTGGCTCAAAGAGAAGAGCAAAAAAAGATTGATGGAGCTGAACCT
  CTTACACCAGAAGAGACTGAAGAAAAGGAAAAACTTCTCACACAAGGTTTCACAAACTGGACTAAACGAG
  ATTTTAACCAGTTTATTAAAGCTAATGAGAAATATGGAAGAGATGACATTGATAACATAGCTCGAGAGGT
  AGAGGGCAAATCCCCTGAGGAGGTCATGGAGTATTCAGCTGTATTTTGGGAACGTTGCAATGAATTACAG
  GACATTGAGAAAATTATGGCTCAAATTGAACGTGGAGAAGCAAGAATTCAACGAAGGATCAGTATCAAGA
  AAGCCCTGGATGCCAAAATTGCAAGATACAAGGCTCCATTTCATCAGTTGCGCATTCAGTATGGAACCAG
  CAAAGGAAAGAACTATACTGAGGAAGAAGATAGATTCTTGATTTGTATGTTACACAAAATGGGCTTTGAT
  AGAGAAAATGTATATGAAGAATTAAGACAGTGTGTACGAAATGCTCCCCAGTTTAGATTTGACTGGTTTA
  TCAAGTCTAGGACTGCCATGGAATTCCAGAGACGCTGTAACACTCTGATTTCATTGATTGAGAAAGAAAA
  TATGGAAATTGAGGAAAGAGAGAGAGCAGAAAAGAAGAAACGGGCAACTAAAACTCCAATGGTAAAATTT
  TCAGCATTTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218308 representing NM_003069
  Red=Cloning site Green=Tags(s)
  
  MEQDTAAVAATVAAADATATIVVIEDEQPGPSTSQEEGAAAAATEATAATEKGEKKKEKNVSSFQLKLAA
  KAPKSEKEMDPEYEEKMKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLGRPRIKKDEKQSLIS
  AGDYRHRRTEQEEDEELLSESRKTSNVCIRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGILADEM
  GLGKTLQTIALLGYLKHYRNIPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDARAAFIRDEMMP
  GEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNNLHEL
  WALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIYLGLS
  KMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVSNSGKMVV
  LDKLLAKLKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYCRLDGQTPHEEREDKFLEVEFLGQREAIEAF
  NAPNSSKFIFMLSTRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEE
  RIVERAEIKLRLDSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFASKESELTDEDITTILERGEKKT
  AEMNERLQKMGESSLRNFRMDIEQSLYKFEGEDYREKQKLGMVEWIEPPKRERKANYAVDAYFREALRVS
  EPKIPKAPRPPKQPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPDIPNPALAQREEQKKIDGAEP
  LTPEETEEKEKLLTQGFTNWTKRDFNQFIKANEKYGRDDIDNIAREVEGKSPEEVMEYSAVFWERCNELQ
  DIEKIMAQIERGEARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFD
  RENVYEELRQCVRNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMVKF
  SAFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003069
• ORF Size: 3162 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003069.5
• RefSeq Size: 4124 bp
• RefSeq ORF: 3165 bp
• Locus ID: 6594
• UniProt ID: P28370
• Cytogenetics: Xq25-q26.1
• Domains: SNF2_N, myb_DNA-binding, DEAD, helicase_C
• Protein Families: Transcription Factors
• MW: 123.1 kDa
• Gene Summary: This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]